Ligninolytic enzyme involved in removal of high molecular weight polycyclic aromatic hydrocarbons by Fusarium strain ZH-H2

Abstract

The capacity of Fusarium sp. strain ZH-H2 to secret lignin peroxidase (LiP), laccase (Lac), and manganese peroxidase (MnP) and degrade high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs) was studied. When the fungus was grown in control mineral salt medium for 4 days, LiP and Lac activities were detected at 8871 U L −1 and 5123 U L −1 , respectively. In the presence of HMW-PAHs as the sole carbon source, only LiP activity was detectable, and LiP activity had significantly reduced HMW-PAHs at day 7, with a maximum decrease of 85.9%. A strong correlation between LiP activity and HMW-PAHs removal efficiency could be fit into various models, with the highest correlation coefficients obtained for quadratic functions (P < 0.01). When a specific enzyme inhibitor was added, the ability of Fusarium to remove HMW-PAHs was reduced from 85.9 to 66.7%, depending on the inhibitor's concentration. Meanwhile, the determined activity of LiP was reduced from 11.4 to 48.6%. We conclude that in the presence of HMW-PAHs as the only carbon source to support growth, Fusarium ZH-H2 mainly produces LiP but not Lac or MnP for HMW-PAHs degradation. To our knowledge, it was the first time to propose a metabolic lignin peroxidase characterization of HMW-PAHs degradation by Fusarium sp. strains.

Isolation, Characterization, and Mycostimulation of Fungi for the Degradation of PAHs at US Superfund Site

“…Their ndings contrasted with those of a previous study (Wu et al 2010), which used a strain of Fusarium solani capable of removing 40% anthracene (ANT) and 60% benz[a]anthracene (BAA) after 40 days. The latter study concluded that the differences between their results, despite using fungi in the same genus, were that different strains created variable effects in the levels, amounts, and types of metabolic enzymes (Zhang et al 2020).…”

“…This variance is the result of these isolates being of the same genus but different strains. The enzymatic capabilities of a speci c strain of Fusarium ZH-H2 vary depending on culture conditions and the presence of PAHs, according to one study (Zhang et al 2020). According to the study's ndings, the strain could produce both lignin perox-idase (LiP) and laccase in mineral salt medium, but when exposed to HMW-PAHs, only LiP was secreted and detected.…”

Bacteria, Fungi and Microalgae for the Bioremediation of Marine Sediments Contaminated by Petroleum Hydrocarbons in the Omics Era

“…The degradation of PAHs by ligninolytic fungi have been extensively studied in the past few years [ 151 ], especially in white rot fungi. These fungi produce extracellular peroxidases which are responsible for the initial oxidation of PAHs [ 152 ]. The principal mechanism of biodegradation used by white rot fungi relies on the lignin degradation system which involves extra-cellular lignin modifying enzymes (LMEs), displaying reduced substrate-specificity.…”

Lignocellulolytic Enzymes Production by Four Wild Filamentous Fungi for Olive Stones Valorization: Comparing Three Fermentation Regimens

“…Penicillium , Fusarium , Trichoderma , and Aspergillus genera belonging to the Ascomycota division, are well-known filamentous fungi capable of producing extracellular enzymes. Their crucial role in efficiently breaking down complex plant cell wall components has been extensively studied and optimized at different scales [ 33 - 36 ]. However, the development and widespread industrial use of lignocellulolytic enzymes face several limitations and challenges.…”