Ligninolytic activity from newly isolated basidiomycete strains and effect of these enzymes on the azo dye orange II decolourisation

Abrahão, Maira Cortellini; Gugliotta, Adriana de Mello; Silva, Roberto da; Fujieda, Roberto Joanne Yashihiro; Boscolo, Maurício; Gomes, Eleni

doi:10.1007/bf03175538

Cited by 19 publications

(11 citation statements)

References 31 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the trends in extracellular enzyme activity differed significantly among enzymes. Lip, laccase and Mnp all belongs to ligninolytic enzymes [ 50 ]. The optimum temperature of laccase was 20 °C in our study, when its activity was highest on the 10th day of cultivation.…”

Section: Discussionmentioning

confidence: 99%

Influence of Temperature on the Bacterial Community in Substrate and Extracellular Enzyme Activity ofAuricularia cornea

Zhang

Miao

et al. 2018

Mycobiology

View full text Add to dashboard Cite

Temperature is an important environmental factor that can greatly influence the cultivation of Auricularia cornea. In this study, lignin peroxidase, laccase, manganese peroxidase, and cellulose in A. cornea fruiting bodies were tested under five different temperatures (20 °C, 25 °C, 30 °C, 35 °C, and 40 °C) in three different culture periods (10 days, 20 days and 30 days). In addition, the V4 region of bacterial 16S rRNA genes in the substrate of A. cornea cultivated for 30 days at different temperatures were sequenced using next-generation sequencing technology to explore the structure and diversity of bacterial communities in the substrate. Temperature and culture days had a significant effect on the activities of the four enzymes, and changes in activity were not synchronized with changes in temperature and culture days. Overall, we obtained 487,694 sequences from 15 samples and assigned them to 16 bacterial phyla. Bacterial community composition and structure in the substrate changed when the temperature was above 35 °C. The relative abundances of some bacteria were significantly affected by temperature. A total of 35 genera at five temperatures in the substrate were correlated, and 41 functional pathways were predicted in the study. Bacterial genes associated with the membrane transport pathway had the highest average abundance (16.16%), and this increased at 35 °C and 40 °C. Generally, different temperatures had impacts on the physiological activity of A. cornea and the bacterial community in the substrate; therefore, the data presented herein should facilitate cultivation of A. cornea.

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of Temperature on the Bacterial Community in Substrate and Extracellular Enzyme Activity ofAuricularia cornea

Zhang

Miao

et al. 2018

Mycobiology

View full text Add to dashboard Cite

show abstract

“…Decolorization of the azo dye direct blue 14 was monitored in a mixture containing 1.4 mL DB14 solution (20 mg L −1 ), 0.2 mL 0.5 M sodium acetate buffer pH 3.5, 0.2 mL crude enzyme, 0.2 mL of deionised water, 0.1 M H 2 O 2 , 0.1 M MnSO 4 or 5.5 mM ABTS solutions, at 25 ± 2°C [20]. …”

Section: Methodsmentioning

confidence: 99%

Bioremediation of Direct Blue 14 and Extracellular Ligninolytic Enzyme Production by White Rot Fungi:PleurotusSpp.

Singh

Vishwakarma

Srivastava

2013

BioMed Research International

View full text Add to dashboard Cite

In the present investigation, four species of white rot fungi (Pleurotus), that is, P. flabellatus, P. florida, P. ostreatus and P. sajor-caju were used for decolorization of direct blue 14 (DB14). Among all four species of Pleurotus, P. flabellatus showed the fastest decolorization in petri plates on different concentration, that is, 200 mg/L, 400 mg/L, and 600 mg/L. All these four species were also evaluated for extracellular ligninolytic enzymes (laccase and manganese peroxidase) production and it was observed that the twelve days old culture of P. flabellatus showed the maximum enzymatic activity, that is, 915.7 U/mL and 769.2 U/mL of laccase and manganese peroxidase, respectively. Other three Pleurotus species took more time for dye decolorization and exhibited less enzymatic activities. The rate of decolorization of DB14 dye solution (20 mg/L) by crude enzymes isolated from P. flabellatus was very fast, and it was observed that up to 90.39% dye solution was decolorized in 6 hrs of incubation.

show abstract

“…A significant influence of incubation temperature on ligninolytic enzymes of Pleurotus sp. and Dichomitussqualen and other white rot fungi has been reported, and temperatures ranging from 25 to 30ºC were found optimum for laccase production 58,59,61,62 . Shanmugam et al, 42 have reported the Pleurotuseous showed highest laccase production at temperature 50 ºC.…”

Section: Graph 4: Effect Of Temperature On Laccase Production By Maramentioning

confidence: 96%

“…Shanmugam et al, 42 have reported the Pleurotuseous showed highest laccase production at temperature 50 ºC. Abrahão et al, 62 have reported a class of isolated Basidiomycetes with an optimum laccase activity at 70ºC.…”

Section: Graph 4: Effect Of Temperature On Laccase Production By Maramentioning

confidence: 99%

Untitled

2016

IJPSR

View full text Add to dashboard Cite

ABSTRACT:The aim of the present study was to investigate the production of laccase by Marasmius sp. BBKAV79 in Solid State Fermentation (SSF) and its antiproliferative activity. Experiments were also conducted to determine the variables that could affect activity of laccase. In particular, the effects of the initial moisture content, pH, temperature, and inoculum volume, incubation period in addition to carbon and nitrogen sources were evaluated. Rice bran was found to be the best supported lignocellulosic substrate for extracellular laccase production under SSF. Highest activity of laccase was achieved at pH 6.0 and temperature at 40°C. Inoculum size and incubation period for laccase production at 2000µl culture broth and 10 days respectively. The presence of starch and ammonium sulphate in the growth medium was found to be favourable for the production of the laccase. Such a high activity was obtained without any addition of inducers. Thus, the indigenous isolate is found to be a potential producer of laccase using SSF and can be exploited for further biotechnological applications. The process also promises economical utilization and value addition of agroresidues and it potently suppressed the proliferation of tumor cell lines KB, MCF7 and VERO with an IC50 value of 88.61 µl, 49.5 µl, and ~67603 µl respectively, signifying that it is an anticancer protein.

show abstract

Ligninolytic activity from newly isolated basidiomycete strains and effect of these enzymes on the azo dye orange II decolourisation

Cited by 19 publications

References 31 publications

Influence of Temperature on the Bacterial Community in Substrate and Extracellular Enzyme Activity ofAuricularia cornea

Influence of Temperature on the Bacterial Community in Substrate and Extracellular Enzyme Activity ofAuricularia cornea

Bioremediation of Direct Blue 14 and Extracellular Ligninolytic Enzyme Production by White Rot Fungi:PleurotusSpp.

Untitled

Contact Info

Product

Resources

About