Ligninolytic ability and potential biotechnology applications of the South American FungusPleurotus laciniatocrenatus

Abstract

The extracellular ligninolytic enzyme system of Pleurotus laciniatocrenatus, grown under different culture conditions, was characterized and the ability of this strain to degrade different components of Eucalyptus globulus wood was determined. In shaken liquid cultures grown on a C-limited medium supplemented with yeast extract (0.1%) and peptone (0.5%), the fungus produced extracellular aryl-alcohol oxidase (Aao), laccase (Lac), manganese-dependent peroxidase (MnP) and manganese-independent peroxidase (MiP) activities, their maximum levels being, respectively, about 600, 50, 1360, and 920 pkat/mL. The supplementation of 1 mmol/L vanillic acid and 150 micromol/L CuSO4 produced an increase of Lac activity levels up to 4-fold and 68.3-fold, respectively. No significant differences were found in the levels of the other ligninolytic enzyme activities when compared to the basal medium. Solid-state fermentation cultures on E. globulus wood chips revealed Lac and MiP activities. These cultures showed degradative activity on lignin and lipophilic wood extractives.

Plant Growth Promoting Rhizobacteria: Fundamentals and Applications

“…Among these enzymes can be highlighted chitinases, lipoxygenases, peroxidases, and glucanases. Plants express the activity of peroxidase during pathogen-host interaction (Saikia et al 2006), where this enzyme has been implicated in the oxidation of phenols (Schmid and Feucht 1980), lignification (Saparrat and Guillen 2005), plant protection (Hammerschmidt et al 1982), and elongation of plant cells (Goldberg et al 1986). Increased activity of peroxidase has been correlated with resistance in many plant species, including rice and wheat (Young et al 1995).…”

Screening for alkaline keratinolytic activity in fungi isolated from soils of the biosphere reserve “Parque Costero del Sur” (Argentina)

“…Screening for fungal growth at alkaline pH All fungi isolated were inoculated according to Saparrat and Guillén (2005) in a basal liquid medium as reported by Rojas et al (2009), supplemented with ground soya meal (SM, 250-1,000 lm particles) at 1.5% (w v -1 ) and buffer-solutions according to Nagai et al (1995) to adjust the pH to 9.0. Three replicates were incubated for 15 days at 28 ± 1.5°C under shaking (200 rpm).…”

Diversity of Bacterial and Fungal Communities in Wheat Straw Compost for Agaricus bisporus Cultivation

“…Microbial activity leads to temperature increments, as their metabolic activity produces energy by the codigestion of lignocellulosic biomass. In addition, ligninolytic fungi produce highly active ligninolytic enzymes, which further delignify the plant biomass ( Cilerd zi c et al, 2017;Rouches et al, 2017;Saparrat and Guill en, 2005;Snajdr and Baldrian, 2006). A study confirmed that the micro-organisms degrade about 40% of the dry matter of in the compost, and the dry matter holds potential valuable nutrients of A. bisporus (Straatsma et al, 1994).…”