Lignin plays a negative role in the biochemical process for producing lignocellulosic biofuels

Zeng, Yining; Zhao, Shuai; Yang, Shihui; Ding, Shi You

doi:10.1016/j.copbio.2013.09.008

Cited by 432 publications

(271 citation statements)

References 40 publications

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“…1, 2), which was attributed to the presence of lignin (Chimenez et al 2014), hydrolysis of the Fibre was quite rapid. Lignin is known to have a negative impact on enzymatic hydrolysis (Zeng et al 2014), which may in part account for the slower hydrolysis of the Fibre than AT fibre. The Fibre was observed to split into elongated parts during hydrolysis.…”

Section: Resultsmentioning

confidence: 99%

Visualization of structural changes in cellulosic substrates during enzymatic hydrolysis using multimodal nonlinear microscopy

et al. 2016

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Enzymatic hydrolysis of cellulose provides a renewable source of monosaccharides for production of variety of biochemicals and biopolymers. Unfortunately, the enzymatic hydrolysis of cellulose is often incomplete, and the reasons are not fully understood. We have monitored enzymatic hydrolysis in terms of molecular density, ordering and autofluorescence of cellulose structures in real time using simultaneous CARS, SHG and MPEF microscopy with the aim of contributing to the understanding and optimization of the enzymatic hydrolysis of cellulose. Three celluloserich substrates with different supramolecular structures, pulp fibre, acid-treated pulp fibre and Avicel, were studied at microscopic level. The microscopy studies revealed that before enzymatic hydrolysis Avicel had the greatest carbon-hydrogen density, while pulp fibre and acid-treated fibre had similar density. Monitoring of the substrates during enzymatic hydrolysis revealed the double exponential SHG decay for pulp fibre and acid-treated fibre indicating two phases of the process. Acid-treated fibre was hydrolysed most rapidly and the hydrolysis of pulp fibre was spatially non-uniform leading to fractioning of the particles, while the hydrolysis of Avicel was more than an order of magnitude slower than that of both fibres.

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Section: Resultsmentioning

confidence: 99%

Visualization of structural changes in cellulosic substrates during enzymatic hydrolysis using multimodal nonlinear microscopy

et al. 2016

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“…It is a promising substrate for production of molecules like ethanol and lactic acid; however, lignin inhibits the action of many common enzymes by sequestering the cellulose and xylose. This creates a bottleneck in efficient bio-production from this material [34]. Proteomics is at the forefront of deciphering solutions to this problem, either through fully integrated systems analysis or assessment of solutions utilising multiple organisms simultaneously.…”

Section: Case Study: Lignocellulose Degradationmentioning

confidence: 99%

Advances in proteomics for production strain analysis

Landels

Evans

Noirel

et al. 2015

Current Opinion in Biotechnology

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Highlights Proteomics is widely used in production strain analysis  The value of specific strategies is discussed with reference to case studies  Methodologies often based on prior application to eukaryotic systems  New developments target quantitative accuracy and proteome coverage AbstractProteomics is the large-scale study and analysis of proteins, directed to analysing protein function in a cellular context. Since the vast majority of the processes occurring in a living cell rely on protein activity, proteomics offer a unique vantage point from which researchers can dissect, characterise, understand and manipulate biological systems. When developing a production strain, proteomics offers a versatile toolkit of analytical techniques. In this commentary, we highlight a number of recent developments in this field using three industrially relevant case studies: targeted proteomic analysis of heterologous pathways in E. coli, biofuel production in Synechocystis PCC6803 and proteomic investigations of lignocellulose degradation. We conclude by discussing future developments in proteomics that will impact upon metabolic engineering and process monitoring of bio-producer strains.

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“…Carbohydrates (cellulose and hemicelluloses) can be hydrolyzed into fermentable sugars and further processed into bioethanol and other biofuels (Zeng et al 2014). However, carbohydrate bioconversion is hindered by the complex structure and distribution of heterogeneous components within cell walls.…”

Section: Introductionmentioning

confidence: 99%

Topochemical Correlation between Carbohydrates and Lignin in Eucommia ulmoides Cell Wall from Tissue to Cell Level

et al. 2016

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The efficient conversion of biomass into biofuels is closely associated with the topochemistry of the cell wall. In this study, the topochemical correlation between carbohydrates and lignin in the Eucommia ulmoides cell wall was investigated in situ by confocal Raman microscopy. The carbohydrates and lignin were mainly collocated in the secondary wall of the fiber, ray parenchyma, and vessel in E. ulmoides. High carbohydrates were associated with low lignin or vice versa, indicating that a high concentration of carbohydrates leads to a drop in the degree of lignification. Furthermore, the band intensity ratio of S-and G-lignin to carbohydrates (I1333/I2889 and I1274/I2889) in morphologically distinct regions of fiber was calculated. In accordance with the wet chemical analysis, a higher ratio of lignin to carbohydrates was observed within the middle layer of the 3-year-old E. ulmoides fiber secondary wall. The results potentially extend the current understanding of the carbohydrate and lignin topochemistry in woody biomass and may facilitate an efficient wood bioconversion process in future biorefineries.

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Lignin plays a negative role in the biochemical process for producing lignocellulosic biofuels

Cited by 432 publications

References 40 publications

Visualization of structural changes in cellulosic substrates during enzymatic hydrolysis using multimodal nonlinear microscopy

Visualization of structural changes in cellulosic substrates during enzymatic hydrolysis using multimodal nonlinear microscopy

Advances in proteomics for production strain analysis

Topochemical Correlation between Carbohydrates and Lignin in Eucommia ulmoides Cell Wall from Tissue to Cell Level

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