Lignin-Modifying Enzymes From the White-Rot Fungus Phlebia Radiata

Paavola, M.-L. Niku; Karhunen, Eija; Kantelineu, A.; Viikari, Liisa

doi:10.1016/b978-0-409-90192-4.50046-7

Cited by 10 publications

(10 citation statements)

References 12 publications

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“…Some properties of Mn-peroxidases P. ostreG~u8 were investigated for identification of isolated enzymes, pH-optimum of isoenzymes of Mn-peroxidase from P. o8~r'eatt~s was equal to 4.5. The same pH-optimum was observed for Mn-peroxidases from other fungi (Niku-Paavola et a~., 1990;Johansson et GZ., 1990;Paszozynski et a~., 1986). pH-Optimum of Mn-peroxidase from B. osfreGttas was obtained for reactions of Mn z° oxidation and Mn z°-dependent oxidation of syringaldazine.…”

Section: Resultssupporting

confidence: 54%

Mn-peroxidase from Pleurotus ostreatus: The action on the lignin

Becker

Sinitsyn

1993

Biotechnol Lett

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Section: Resultssupporting

confidence: 54%

Mn-peroxidase from Pleurotus ostreatus: The action on the lignin

Becker

Sinitsyn

1993

Biotechnol Lett

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“…One U/ml of MnP was defined as 1 lmol of Mn(II) oxidized to Mn(III) per min. Laccase activity was measured by the oxidation of 2, 2 0 -azinodi-3-ethyl-benzothiazoline-6-sulfuric acid (ABTS) at 436 nm (Niku-Paavola et al 1988). The reaction mixture contains 10 mM ABTS, 85 mM sodium tartrate buffer (pH 3.0) and enzyme.…”

Section: Estimation Of Enzymes For Decolorizationmentioning

confidence: 99%

Isolation of fungi and optimization of process parameters for decolorization of distillery mill effluent

Kaushik

Thakur

2009

World J Microbiol Biotechnol

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Five different fungi isolated from distillery mill site in which two isolates (DF3 and DF4) had higher capabilities to remove color were identified as Emericella nidulans var. lata and Neurospora intermedia, respectively. Optimization of process parameter for decolorization was initially performed to select growth factors which were further substantiated by Taguchi approach in which seven factors, %carbon, %nitrogen, duration, pH, temperature, stirring and inoculum size, at two levels applying L-8 orthogonal array were taken for both fungi. Maximum color was removed at pH 3, temperature 30°C, stirring 125 rpm, dextrose (0.05%) and sodium nitrate (0.025%) by both fungi. After optimization, there was two-fold increase in color removal from 38 to 62% (DF3) and 31 to 64% (DF4) indicating significance of Taguchi approach in decolorization of distillery mill effluent. The mechanism for decolorization was determined by enzyme analysis, laccase and glucose oxidase, and indicated significant activity in DF3 as compared to DF4.

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“…Activity of MnP was determined by measuring the rate of oxidation of veratryl alcohol to veratraldehyde in the presence of Mn and H 2 O 2 (Paszczynski et al, 1988). Laccase was assayed by measuring oxidation of 1 mM ABTS (2,2 0 -azino-bis-(3-ethylbenzothazoline-6-sulfonate) at 405 nm (Niku-Paavola et al, 1988). Units were calculated as lM of the substrate transformed min À1 l À1 of culture supernatants and expressed as U l À1 .…”

Section: Decolorization By the Whole-fungal Culture And Partially Purmentioning

confidence: 99%

A novel hybrid technology for remediation of molasses-based raw effluents

Verma

Raghukumar

Naik

2011

Bioresource Technology

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A novel three-step technology for treatment of four molasses-based raw industrial effluents, varying in their COD, color and turbidity is reported here. Sequential steps involved in this treatment are; (1) sonication of the effluents, (2) whole-fungal treatment of these by a ligninolytic marine fungus and (3) biosorption of the residual color with heat-inactivated biomass of the same fungus. Sonication reduced the foul odor and turbidity of the effluents. It increased biodegradability of the effluents in the second stage of treatment. Laccase production in the presence of all the four effluents was directly correlated with their decolorization. After the third step, a reduction of 60-80% in color, 50-70% in COD and 60-70% in total phenolics were achieved. Comparative mass and nuclear magnetic resonance spectra indicated increasing degradation of the effluent components after each stage. Toxicity (LC(50) values) against Artemia larvae was reduced by two to five folds.

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Lignin-Modifying Enzymes From the White-Rot Fungus Phlebia Radiata

Cited by 10 publications

References 12 publications

Mn-peroxidase from Pleurotus ostreatus: The action on the lignin

Mn-peroxidase from Pleurotus ostreatus: The action on the lignin

Isolation of fungi and optimization of process parameters for decolorization of distillery mill effluent

A novel hybrid technology for remediation of molasses-based raw effluents

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