Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28

Abstract

SUMMARYThe aim of this research is to isolate and identify fungi with high lignin-degrading abilities that are autochthonous to southern Serbian region. Two novel fungal isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 were selected to assess their ligninolytic enzyme activities and the efficiency of lignin removal from beech wood sawdust. Obtained results show that both isolates are good sources of industrially valuable enzymes with a potential for application in various biotechnological and industrial processes. Both isolates showed laccase, manganese-dependent peroxidase, and versatile peroxidase activities, while only S. gausapatum F28 had lignin peroxidase activity. This is the first record of the ability of S. gausapatum species to produce lignin peroxidase. T. hirsuta F13 showed higher laccase activity than S. gausapatum F28, while S. gausapatum F28 had higher manganese peroxidase activity. Also, T. hirsuta F13 exhibited much higher laccase activity under submerged cultivation conditions than solid-state cultivation conditions, which is rare for fungi. This is important for industrial processes since the submerged fermentation is a dominant technique in industry. The test of the efficiency of lignin removal showed that both isolates are efficient lignin decomposers. After five weeks of incubation on beech wood sawdust, the total lignin losses were 33.84% with T. hirsuta F13 and 28.8% with S. gausapatum F28.

A window into fungal endophytism in Salicornia europaea: deciphering fungal characteristics as plant growth promoting agents

“…It is a basidiomycetous genus and some of its species were obtained as endophytes of coastal grasses and forest trees Theobroma gileri (Márquez et al 2008;Thomas et al 2008). Lastly, S. gausapatum collected from oak forest and orchards in southern Serbia displayed high lignin-degrading abilities and the efficiency of lignin removal from beech wood sawdust (Jović et al 2018). Not much information was reported on the plant growth promoting abilities of this strain.…”

Pretreatment of lignocellulosic biomass with autochthonous fungi from Serbia

“…The three isolates with the best lignocellulolytic enzyme activities were identified using ITS sequences, and the sequences were deposited in the NCBI GenBank database. Their accession numbers are KY264754.1 (Trametes hirsuta F13), KY264753.1 (Stereum gausapatum F28), and MF521930.1 (Myrmaecium fulvopruinatum F28) (Jović et al, 2018). Sugar beet MS was obtained from a local alcohol industry, and beechwood sawdust was obtained from a local sawmill.…”

The agricultural waste utilization for exploration of the extracellular enzyme potential of three novel white-rot Basidiomycetes: Fomes fomentarius TMF2, Schizophyllum commune TMF3 and Bjerkandera adusta TMF1

“…Molecular identi cation of fungal isolates DNA extraction and PCR ampli cation were carried out according to the procedure described by Jović and coworkers with some modi cations [14]. For PCR ampli cation of the internal transcribed spacer (ITS) region of the ribosomal RNA coding DNA, a primer set ITS1/ITS4 was used.…”

“…The PCR ampli cation was performed in QB24 Thermal Cycler (Quanta Biotech Ltd., By eet, UK). The temperature parameters were set as follows: 15 min at 95 °C forinitial denaturation, 30 cycles of denaturation step (30 s at 95°C), annealing step (30 s at 55 °C) and elongation step (90 s at72 °C), and 5 min at 72 °C for nal extension [14].…”

“…The determination of laccase activity was based on the oxidation of guaiacol. This assay was performed according to the procedure described by Jović et al [14].…”