Lignification and Related Enzymes in Glycine max Root Growth-Inhibition by Ferulic Acid

Santos, Wanderley Dantas dos; Ferrarese, Maria de Lourdes Lúcio; Finger, Aline; Teixeira, Aline Cristina Neres; Ferrarese-Filho, Osvaldo

doi:10.1023/b:joec.0000030272.83794.f0

Cited by 105 publications

(74 citation statements)

References 11 publications

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“…Another nine, including multifunctional class III peroxidase (POD) 79, POD 78 with two isoforms (spots P444/G245 and P702/G699), POD 31 with two isoforms (spots P605/G326 and P194/G45), polygalacturonase Os02g10300 with two isoforms (spots -/g242 and -/g281), and expansin with two sequence-related isoforms (Os10g40090, spot -/g93; Os03g01640, spot -/g398), may be responsible for wall loosening and hydrolyzing. Class III PODs have been documented to function in promoting elongation of Arabidopsis roots (31,32) or physically inhibiting the elongation process by creating cell wall cross-linking (33). Polygalacturonase is a key enzyme-degrading pectin, and expansins are involved in cell elongation growth.…”

Section: Identification Of Proteins Involved In Pollen Germinationmentioning

confidence: 99%

Proteomics Identification of Differentially Expressed Proteins Associated with Pollen Germination and Tube Growth Reveals Characteristics of Germinated Oryza sativa Pollen

Dai

Chen

Chong

et al. 2007

Molecular & Cellular Proteomics

138

134

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Mature pollen from most plant species is metabolically quiescent; however, after pollination, it germinates quickly and gives rise to a pollen tube to transport sperms into the embryo sac. Because methods for collecting a large amount of in vitro germinated pollen grains for transcriptomics and proteomics studies from model plants of Arabidopsis and rice are not available, molecular information about the germination developmental process is lacking. Here we describe a method for obtaining a large quantity of in vitro germinating rice pollen for proteomics study. Two-dimensional electrophoresis of ϳ2300 protein spots revealed 186 that were differentially expressed in mature and germinated pollen. Most showed a changed level of expression, and only 66 appeared to be specific to developmental stages. Furthermore 160 differentially expressed protein spots were identified on mass spectrometry to match 120 diverse protein species. These proteins involve different cellular and metabolic processes with obvious functional skew toward wall metabolism, protein synthesis and degradation, cytoskeleton dynamics, and carbohydrate/energy metabolism. Wall metabolism-related proteins are prominently featured in the differentially expressed proteins and the pollen proteome as compared with rice sporophytic proteomes. Our study also revealed multiple isoforms and differential expression patterns between isoforms of a protein. These results provide novel insights into pollen function specialization. Pollen of flowering plants, generated in diploid sporophytic plants via meiosis followed by two cycles of mitosis, contains three haploid genomes and is a highly reduced organism. Mature pollen grains from most plant species are metabolically quiescent. However, during pollination, they can quickly germinate and give rise to a polarly growing pollen tube whereby the pollen interacts with pistils and then delivers two sperms into the embryo sac to initiate double fertilization. Besides having biological importance, pollen germination and tube growth have been considered unique developmental processes for studying cell polar establishment, cell differentiation, cell fate determination, and cell-to-cell recognition. Thus, the molecular mechanisms underlining the specific cellular programs have been the focus of investigation over the past 50 years (1). However, until now, only a limited number of genes encoding coat/wall proteins or signal molecules have been shown to be essential for pollen germination, tube growth, and interaction of the tube and stigma (1-7).Recent analyses of mature pollen of Arabidopsis revealed the transcriptome to have reduced complexity and a higher proportion of selectively expressed transcripts than sporophytic tissues (8 -10). As well, about one-third of the genes expressed in vegetative tissues are not expressed in the pollen (8). The observation suggests that the transcriptional characteristics involve pollen function specialization. Furthermore these studies determined that pollen transcriptome has a functiona...

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Section: Identification Of Proteins Involved In Pollen Germinationmentioning

confidence: 99%

Proteomics Identification of Differentially Expressed Proteins Associated with Pollen Germination and Tube Growth Reveals Characteristics of Germinated Oryza sativa Pollen

Dai

Chen

Chong

et al. 2007

Molecular & Cellular Proteomics

138

134

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“…PAL was extracted and its activity was determined using the HPLC method described by Dos Santos et al (2004), with two modifications as previously described (Kováčik et al, 2007). Briefly, samples were ground in 0.1 M sodium borate buffer and centrifuged at 48C.…”

Section: Determination Of Cadmium and Copper Content In Tissuementioning

confidence: 99%

“…The assay mixture (3.0 mL) contained 50 mM phosphate buffer (pH 7.0), 2.58 mM guaiacol, 10 mM H 2 O 2 , and supernatant. Guaiacol oxidation to tetraguaiacol was followed for 5 min at 470 nm as described by Dos Santos et al (2004). GPX activity was expressed as lmol tetraguaiacol min 21 mg 21 protein.…”

Section: Determination Of Cadmium and Copper Content In Tissuementioning

confidence: 99%

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Physiological responses of Matricaria chamomilla to cadmium and copper excess

Kováčik

Bačkor

Kaduková

2008

Environmental Toxicology

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Physiological responses of Matricaria chamomilla plants exposed to cadmium (Cd) and copper (Cu) excess (3, 60, and 120 lM for 7 days) with special emphasis on phenolic metabolism were studied. Cu at 120 lM reduced chamomile growth, especially in the roots where it was more abundant than Cd. Notwithstanding the low leaf Cu amount (37.5 lg g 21 DW) in comparison with Cd (237.8 lg g 21 DW) at 120 lM, it caused reduction of biomass accumulation, F v /F m ratio and soluble proteins. In combination with high accumulation of phenolics, strong reduction of proteins and high GPX activity in the roots, this supports severe redox Cu properties. In terms of leaf phenylalanine ammonia-lyase (PAL) activity, it seems that Cd had a stimulatory effect during the course of the experiment, whereas Cu was found to stimulate it after 7-day exposure. The opposite trend was visible in the roots, where Cd had a stimulatory effect at high doses but Cu mainly at the highest dose. This supports the assumption of different PAL time dynamics under Cd and Cu excess. A dose of 60 and 120 lM Cu led to 2-and 3-times higher root lignin accumulation while the same Cd doses increased it by 33 and 68%, respectively. A Cu dose of 120 lM can be considered as limiting for chamomile growth under conditions of present research, while resistance to high Cd doses was confirmed. However, PAL and phenolics seemed to play an important role in detoxification of Cd-and Cu-induced oxidative stress. # 2008 Wiley Periodicals, Inc. Environ Toxicol 23: 123-130, 2008

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“…In general, lignification makes the cell wall firm with concomitant increases in PAL and POD activities. In fact, rise of PAL activity is closely connected to decline of root growth and lignin production in maize, cucumber, and soybean vulnerable to the action of phenylpropanoid allelochemicals (Rama Devi and Prasad 1996;Herrig et al, 2002;dos Santos et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Detection of consensus genomic regions associated with root architecture of bread wheat on groups 2 and 3 chromosomes using QTL meta–analysis

Darzi-Ramandi¹,

Shariati²,

Tavakol³

et al. 2017

Aust J Crop Sci

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Root architecture is an important bread wheat phenomenon that highly influences its production and adaptation to environmental stresses, in particular drought stress. Several QTL studies have been conducted to ascertain chromosomal regions associated with root morphology resulting in identification of various loci depending on evaluated population types and experimental conditions. In order to identify the most consistent and reliable QTLs involved in various root morphological traits in bread wheat, a meta-QTL (MQTL) analysis was performed using 106 QTLs derived from 12 different populations under both normal and drought stress conditions. Among them, 125 QTLs related to root traits were successfully projected onto the reference map and further metaanalysis was focused on chromosomes of homeologous groups 2 and 3 with most assigned QTLs. Consequently, a total of seven MQTLs were identified on chromosomes 2A, 2B, 3A and 3B originated from 2 to 17 initial QTLs with a confidence interval (CI) of 5.3-6.6 to 39.5-55.0 cM. Three MQTLs located on 2A, 3A and 3B derived from 7 to 17 QTLs related to different root morphological traits pointed out the most important chromosomal regions. A reduction in the average 95% confidence interval from 20.8 cM to 6.4 cM was observed when comparing the individual QTL to the MQTL. Further analysis on investigation of candidate genes located in these genomic regions resulted in identification of some genes mainly associated with lignin catabolic process, potassium transporters and leucine-rich repeats receptor-like kinases (LRR-RLKs). These results provid fundamental information on most important genomic regions and candidate genes related to root morphology in bread wheat.

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Lignification and Related Enzymes in Glycine max Root Growth-Inhibition by Ferulic Acid

Cited by 105 publications

References 11 publications

Proteomics Identification of Differentially Expressed Proteins Associated with Pollen Germination and Tube Growth Reveals Characteristics of Germinated Oryza sativa Pollen

Proteomics Identification of Differentially Expressed Proteins Associated with Pollen Germination and Tube Growth Reveals Characteristics of Germinated Oryza sativa Pollen

Physiological responses of Matricaria chamomilla to cadmium and copper excess

Detection of consensus genomic regions associated with root architecture of bread wheat on groups 2 and 3 chromosomes using QTL meta–analysis

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