Lignans from Torreya jackii identified by stopped-flow high-performance liquid chromatography–nuclear magnetic resonance spectroscopy

Yu, Zhao; Nookandeh, Aslieh; Schneider, Bernd; Sun, Xianfeng; Schmitt, Bettina; Stöckigt, Joachim

doi:10.1016/s0021-9673(99)00050-3

Cited by 24 publications

(13 citation statements)

References 16 publications

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“…Such an approach was presented by Bobzin et al (2000) for the determination of marine alkaloids as inhibitors of an enzyme implicated in the type II diabetes. HPLC-NMR was also used for the screening of plant extracts (Bringmann et al 1999(Bringmann et al , 2000(Bringmann et al , 2002Zhao et al 1999). To our knowledge, however, this method has never been used with C. roseus crude extracts.…”

Section: Hplc Methods and Equipmentsmentioning

confidence: 99%

Analysis of Catharanthus roseus alkaloids by HPLC

Hisiger

Jolicœur

2007

Phytochem Rev

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Catharanthus roseus is a medicinal plant from which secondary metabolites used in chemotherapy to treat diverse cancers are extracted. The well known high value metabolites vincristine and vinblastine are just 2 of 130 alkaloids that can be found in C. roseus. However, only few (~11) of this high number of chemical entities are frequently analyzed and even fewer (~8) are available commercially. For more than 30 years, different analytical techniques have been developed to isolate and identify C. roseus metabolites, and then allowing revealing the therapeutic potential of C. roseus metabolites. Among few approaches, high performance liquid chromatography (HPLC) technique is still widely used for the separation and analysis of secondary metabolites such as those from C. roseus. This article thus reviews the most recent developments in HPLC analysis of alkaloids from C. roseus. Diverse considerations that are crucial to the efficiency of secondary metabolites separation and identification steps, such as biomass manipulation, extraction phase and protocols, HPLC separation and analysis protocols are reviewed in details.Examples of spectra obtained using the most common detectors are also shown and suggestions are made on how to proceed in developing efficient separation and identification methods at the analytical and semi-preparative scales.

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Section: Hplc Methods and Equipmentsmentioning

confidence: 99%

Analysis of Catharanthus roseus alkaloids by HPLC

Hisiger

Jolicœur

2007

Phytochem Rev

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“…According to the acquired chemical shifts and comparing with the previously reported NMR spectral data, the structures of two isomeric forms of secoisolariciresinol diglucoside were successfully elucidated using this approach. The other successful applications of on-line NMR on identification of lignans and isoflavones from plant origins have been demonstrated [192][193][194] and summarized by others [189,190].…”

Section: Nuclear Magnetic Resonance Spectroscopymentioning

confidence: 96%

Analytical methods to determine phytoestrogenic compounds

Wang

Simon

2004

Journal of Chromatography B

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“…Lignans are important biologically active phenolic compounds related to lignin, produced in different plants, for example in tribe Cynareae, such as Arctium, (Boldizsár et al, 2010a), Cirsium (Boldizsár et al, 2010b) species, Forsythia (Maiada et al, 1990) or Torreya (Zhao et al, 1999) (Jang et al, 2002). However, the direct effects of arctigenin on normal neuronal activity have not been established.…”

Section: Introductionmentioning

confidence: 99%