Light‐stimulated phosphorylation of proteins in cell‐free extracts from <i>Trichoderma viride</i>

Grešík, Miroslav; Kolarova, N.; Farkaš, Vladimı́r

doi:10.1016/0014-5793(89)80458-2

Cited by 21 publications

(5 citation statements)

References 10 publications

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“…As mentioned above, light induces protein phosphorylation; the addition of cAMP to a cell-free extract of T. viride (now T. atroviride) can mimic the action of light in this process (Gresík et al, 1989). This modification of proteins is a common mechanism of post-translational regulation and is due to the action of protein kinases.…”

Section: Camp-pkamentioning

confidence: 99%

Trichoderma: sensing the environment for survival and dispersal

2012

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Section: Camp-pkamentioning

confidence: 99%

Trichoderma: sensing the environment for survival and dispersal

2012

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“…Light also impacts the cAMP signaling pathway, where it is well established that cAMP as a second messenger is produced by adenylyl cyclase, an enzyme whose activity is modulated by heterotrimeric G-protein ␣-subunits, highlighting the possibility that a photoreceptor coupled to G-proteins (GPCR-like) could be involved in this signaling pathway. Artificially increased intracellular cAMP levels in the dark mimic a light effect in T. atroviride (639) and in T. viride, illumination causes a transient rise in cAMP levels and protein kinase A (PKA)-dependent phosphorylation (640). Additionally, cAMP levels modulate cellulase gene expression (641) and induce coiling (53) in Trichoderma spp.…”

Section: The Camp Pathwaymentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

163

195

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SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

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“…Thereby, illumination causes rapid but transient increase in intracellular concentrations of ATP and cAMP, which might contribute to phosphorylation of proteins in response to light. With respect to phosphorylation, the effect of light can be substituted by addition of 3 mM cAMP (Gresik et al 1989). These cAMP levels could be adjusted by the membrane associated adenylyl cyclase, which is activated by light—in contrast to 3′5′ cyclic AMP phosphodiesterase, for which this is not the case (Kolarova et al 1992).…”

Section: Metabolic Pathways As Output Pathways Of Light Signalingmentioning

confidence: 99%

Light regulation of metabolic pathways in fungi

Tisch

Schmoll

2009

Appl Microbiol Biotechnol

223

190

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Light represents a major carrier of information in nature. The molecular machineries translating its electromagnetic energy (photons) into the chemical language of cells transmit vital signals for adjustment of virtually every living organism to its habitat. Fungi react to illumination in various ways, and we found that they initiate considerable adaptations in their metabolic pathways upon growth in light or after perception of a light pulse. Alterations in response to light have predominantly been observed in carotenoid metabolism, polysaccharide and carbohydrate metabolism, fatty acid metabolism, nucleotide and nucleoside metabolism, and in regulation of production of secondary metabolites. Transcription of genes is initiated within minutes, abundance and activity of metabolic enzymes are adjusted, and subsequently, levels of metabolites are altered to cope with the harmful effects of light or to prepare for reproduction, which is dependent on light in many cases. This review aims to give an overview on metabolic pathways impacted by light and to illustrate the physiological significance of light for fungi. We provide a basis for assessment whether a given metabolic pathway might be subject to regulation by light and how these properties can be exploited for improvement of biotechnological processes.

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Light‐stimulated phosphorylation of proteins in cell‐free extracts from Trichoderma viride

Cited by 21 publications

References 10 publications

Trichoderma: sensing the environment for survival and dispersal

Trichoderma: sensing the environment for survival and dispersal

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Light regulation of metabolic pathways in fungi

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