Light-start DNA amplification using light-controlled DNA polymerase

Cai, Tingting; Zhong, Cai-ling; He, Yide; Chen, Yao-Qing; Wang, Junqing; Wang, Jiaqi; Zheng, Ke-wei

doi:10.1016/j.snb.2023.133797

Cited by 3 publications

(3 citation statements)

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Section: Photo-modulated Nucleic Acid Amplificationmentioning

confidence: 99%

“…To address this challenge and develop more versatile methods for NAA regulation, some studies have explored the direct coupling of oligonucleotide chains with photosensitive properties onto DNA polymerases for the photoactivation regulation of NAA. 88c, 91 For example, Vera's team achieved the regulation of PCR reactions by covalently linking an oligonucleotide sequence directly to the Taq enzyme via click chemistry reaction (Figure 8E). 88c The authors demonstrated the general applicability of this strategy to various DNA processing enzymes, such as Phi29, Taq, Pfu polymerase and restriction enzyme activity.…”

Section: Photo-modulated Nucleic Acid Amplificationmentioning

confidence: 99%

“…Regulation of DNA polymerase activity was also achieved by fusing G quadruplex-binding proteins and utilizing DNA aptamers containing G quadruplexes, hairpin structures, and PC-linker as blockers (Figure 8F). 91 The G quadruplex in the DNA aptamer tightly binds to the G quadruplex binding protein, which allows the hairpin structure to occupy the active site of the DNA polymerase. The bond of the PC-linker in the DNA aptamer was broken under UV illumination, which ultimately activates the activity of enzymes.…”

Section: Photo-modulated Nucleic Acid Amplificationmentioning

confidence: 99%

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Photochemical regulatory strategies for nucleic acid function and their biomedical applications

Hu,

Wang,

Zhou

2024

Interdisciplinary Medicine

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Nucleic acids are not only essential biomolecules that drive critical life processes such as growth, development, reproduction, inheritance, and mutation, but also serve as significant markers for disease diagnosis, pathogen identification, and cancer screening. Nevertheless, several challenges have hindered the widespread use of nucleic acids in biomedicine, such as susceptibility to degradation, limited cellular uptake efficiency, potential toxicity, and uncontrollable activity. Photo‐regulation offers an effective solution to address these challenges. It allows for the precise control of nucleic acid structure and function and enhances the stability and safety of their application in biomedicine. In this review, we systematically review the structural characteristics of the three primary photosensitive groups commonly used in the regulation of nucleic acid molecules (i.e., photocleavable molecules, photoisomerization molecules, and photo‐crosslinking molecules) under light irradiation. Subsequently, recent research advances in the development and application of photo‐modulation strategies based on these photosensitive molecules in antisense oligonucleotides, RNA interference, nucleic acid amplification, and CRISPR/Cas systems are outlined. Finally, we discuss the challenges faced in the widespread application of these photo‐regulatory strategies and outline potential future directions for their development.

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Section: Photo-modulated Nucleic Acid Amplificationmentioning

confidence: 99%

Section: Photo-modulated Nucleic Acid Amplificationmentioning

confidence: 99%

Section: Photo-modulated Nucleic Acid Amplificationmentioning

confidence: 99%

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