Light-sheet microscopy in the near-infrared II window

Abstract

Deep-tissue three-dimensional (3D) optical imaging of live mammals with high spatiotemporal resolution in non-invasive manners has been challenging due to light scattering. Here, we developed near-infrared II (NIR-II, 1000–1700 nm) light sheet microscopy (LSM) with excitation and emission up to ~ 1320 nm and ~ 1700 nm respectively for optical sectioning through live tissues at ~ 750-μm penetration depth without any invasive surgery. Suppressed light scattering allowed imaging at ~ 2 mm depth in glycerol-cleared brain tissues. NIR-II LSM in normal and oblique configurations enabled in vivo imaging of live mice through intact tissue, revealing abnormal blood flow and T cell motion in tumor microcirculation and mapping out programmed-death ligand 1 (PD-L1) and programmed cell death protein 1 (PD-1) in tumors with cellular resolution. 3D imaging through intact mouse head resolved vascular channels between skull and brain cortex, and monitored recruitment of macrophages/microglia to traumatic brain injury site post injury.

Multiplexed NIR‐II Probes for Lymph Node‐Invaded Cancer Detection and Imaging‐Guided Surgery

“…Using NIR‐II fluorescence imaging at longer wavelengths with reduced scattering and tissue autofluorescence is beneficial across nearly all tissue types . Our study further indicated that imaging in the NIR‐IIb window yielded greater spatial resolution and penetration depth, considering the combined effects of minimized photon scattering and water absorption.…”

A Review on Microscopic Visual Servoing for Micromanipulation Systems: Applications in Micromanufacturing, Biological Injection, and Nanosensor Assembly

“…Micromachines 2019, 10, 843 14 of 20 (9) At present, a time-efficient, non-invasive, environmentally compatible and high-throughput optical microscopy technique called scanning superlens microscopy (SSUM) has been proposed for large-area, super-resolution imaging and structural information acquisition. This microscopy operates in both non-invasive and contact modes, with 200 times the acquisition efficiency of an atomic force microscopy [91][92][93][94]. It enables large-area observation of live-cell morphology or sub-membrane structures, with sub-diffraction-limited resolution demonstrated by observing biological and non-biological objects.…”

“…(a) A 3D reconstructed image of blood vessels in an intact mouse visualized through the scalp, skull, meninges and brain cortex, obtained 2 h after intravenous injection of PEGylated PbS/CdS core/shell quantum dot (CSQDs) by oblique NIR-II LSM, as shown in (b). (c) shows the 3D time-course light-sheet imaging and monitoring of the dynamics of meningeal macrophages and microglia after brain injury, 24 h after the injection of anti-CD11b PEGylated PbS/CdS CSQDs at the boundary of the traumatic brain injury (TBI) region (courtesy of[93]).…”

Near-infrared fluorescent protein and bioluminescence-based probes for high-resolution in vivo optical imaging

“…9). 152 Several versions of LSFM have been invented with improved resolution in both 2D and 3D imaging. Bessel beam plane illumination microscopy (BBPIM) is the advancement in this line developed by the Eric Betzig group, which used nanometer-thin light sheet illumination.…”