ABSTRACT6-Formylpterin was purified by paper chromatography, and bovine pineal hydroxyindole-O-methyltransferase (S-adenosyl-L-methionine:N-acetylserotonin 0-methyltransferase; EC 2.1.1.4) was purified by flat-bed gel electrofocusing. Fractions of the methyltransferase with isoelectric points at pH 5.0, 5.7, and 5.2 were tested in the presence of 6-formylpterin, and decreased activities were found. Because 6formylpterin is one of the photolytic products of folic acid and decreased serum folic acid has been reported in light-exposed men, we sugest that pteridines may be involved in nonretinal effects of light on pineal melatonin biosynthesis. It may be that this metabolic pathway is an evolutionary relic of primitive light responses of pigmentation mechanism.