Light Scattering by Model Membranes

Earnshaw, J. C.

doi:10.1007/978-1-4684-4487-2_17

Cited by 3 publications

(1 citation statement)

References 35 publications

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“…DLS measurements were conducted using the Zetasizer Nano ZSP (Malvern Instruments, Malvern, UK) system with a fixed scattering angle of 173°. Based on the hydrodynamic radius of unmodified lysozyme being smaller than 10 nm, , isotropic scattering of all investigated species was assumed justifying the measurement at a single scattering angle . The Zetasizer Nano ZS instrument incorporates noninvasive backscattering (NIBS) optics.…”

Section: Methodsmentioning

confidence: 99%

Impact of Polymer Bioconjugation on Protein Stability and Activity Investigated with Discrete Conjugates: Alternatives to PEGylation

et al. 2018

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Covalent attachment of synthetic polymers to proteins, known as protein-polymer conjugation, is currently one of the main approaches for improving the physicochemical properties of these biomolecules. The most commonly employed polymer is polyethylene glycol (PEG), as evidenced by extensive research and clinical track records for its use in biopharmaceuticals. However, the occurrence of allergic reactions or hypersensitivity and the discovery of PEG antibodies, on the one hand, and the rise of controlled polymerization techniques and novel monomers, on the other hand, have been driving the search for alternative polymers for bioconjugation. The present study describes the synthesis, purification, and properties of conjugates of lysozyme with poly( N-acryloylmorpholine) (PNAM) and poly(oligoethylene glycol methyl ether methacrylate) (POEGMA). Particularly, conjugate species with distinct conjugation degrees are investigated for their residual activity, aggregation behavior, and solubility, by using a high-throughput screening approach. Our study showcases the importance of evaluating conjugates obtained by nonsite-specific modification through isolated species with discrete degrees of conjugation rather than on the batch level. Monovalent conjugates with relatively low molar mass polymers displayed equal or even higher activity than the native protein, while all conjugates showed an improved protein solubility. To achieve a comparable effect on solubility as with PEG, PNAM and POEGMA of higher molar masses were required.

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Section: Methodsmentioning

confidence: 99%

Impact of Polymer Bioconjugation on Protein Stability and Activity Investigated with Discrete Conjugates: Alternatives to PEGylation

et al. 2018

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Light Scattering Studies of Interfacial Rheology

Earnshaw¹

1999

Journal of Dispersion Science and Technology

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Waves at liquid surfaces: coupled oscillators and mode mixing

Earnshaw

McLaughlin

1991

Proc. R. Soc. Lond. A

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The dispersion of surface waves on liquids has been reconsidered in the frame work of currently established linear theory. Mixed excitations, which are neither capillary nor dilational in nature, can occur due to the coupling between the lossy oscillator formed by the vertical and horizontal motions of a surface element. The results emphasize that the capillary and dilational waves are only approximately transverse and longitudinal in nature and that it cannot, in general, be correct to neglect the coupling between these degrees of freedom. In the present case mode mixing only occurs when a particular surface viscosity - that affecting shear normal to the surface - exceeds a critical value. Experimentally accessible tests of the predicted mode mixing are proposed, which would further test the physical reality of the surface viscosity involved.

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Light Scattering by Model Membranes

Cited by 3 publications

References 35 publications

Impact of Polymer Bioconjugation on Protein Stability and Activity Investigated with Discrete Conjugates: Alternatives to PEGylation

Impact of Polymer Bioconjugation on Protein Stability and Activity Investigated with Discrete Conjugates: Alternatives to PEGylation

Light Scattering Studies of Interfacial Rheology

Waves at liquid surfaces: coupled oscillators and mode mixing

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