Light-response in two clonal strains of the haptophyte Tisochrysis lutea: Evidence for different photoprotection strategies

Pajot, Anne; Lavaud, Johann; Carrier, Grégory; Lacour, Thomas; Marchal, Luc; Nicolau, Élodie

doi:10.1016/j.algal.2022.102915

Cited by 7 publications

(5 citation statements)

References 58 publications

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“…Moreover, we quantified huge amounts of echinenone, a carotenoid which is known to interact with the Orange-Carotenoid-Protein (OCP) to induce NPQ in cyanobacteria but, which role is still unknown in eukaryotic microalgae. In T. lutea, OCP was not found 28 . Additionally, echinenone does not decrease during dark recovery as Dt does (Figure S3).…”

Section: Xanthophyll Cycle Pigments and Their Involvement In Npqmentioning

confidence: 83%

“…1B) particularly under high light and nutrient stress. Dt and Dd, which has been reported in T. lutea before [27][28][29]47 are generally involved in the so-called xanthophyll cycle (XC). This cycle, found in the main algal classes Bacillariophyceae (diatoms), Xanthophyceae, Haptophyceae, and Dinophyceae 9,48 consists of one de-epoxidation step that converts Dd into Dt within the LHC antennas to convert them in a heat-dissipating state.…”

Section: Xanthophyll Cycle Pigments and Their Involvement In Npqmentioning

confidence: 99%

“…Most studies on the effect of growth conditions on photoprotection were performed under a stable intensity during the light period or even under continuous light (i.e. 24 h Light:0 h Dark with constant light) 15,28 . However, natural light cycle regulates most of the cellular processes of all photosynthetic organisms in the ocean, including cell division, pigment synthesis, nutrient capture and carbon fixation 55,56 .…”

Section: Xanthophyll Cycle Pigments and Their Involvement In Npqmentioning

confidence: 99%

“…Huang, et al 24 reported an increase in slowly relaxing NPQ (hours-time scale, named qNs) in N and P starved cultures of T. lutea, and they interpreted it as enhanced PSII photoinhibition. However, T. lutea and other Isochrysidales species are highly rich in the xanthophyll pigments diadinoxanthin (Dd) and Dt 26 particularly under high irradiance 27,28 and nutrient starvation 29 , suggesting a likely significant involvement of XC-related NPQ-qE in photoprotection. T. lutea also shows many LHCx proteins, particularly expressed under high light 30 that are involved in NPQ-qE in diatoms 31 .…”

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confidence: 99%

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Sustained xanthophyll pigments-related photoprotective NPQ is involved in photoinhibition in the haptophyte Tisochrysis lutea

Lacour,

Robert,

Lavaud

2023

Sci Rep

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Dynamic xanthophyll cycle (XC) related non-photochemical quenching (NPQd, also called qE) is present in most phototrophs. It allows dissipating excess light energy under adverse growing conditions. Generally, NPQd rapidly reverses for photosynthesis to resume when light intensity decreases back toward optimal intensity. Under certain environmental conditions and/or in some species, NPQ can be strongly sustained (NPQs showing hours-to-days relaxation kinetics). Tisochrysis lutea is a South Pacific haptophyte phytoplankton with a strong potential for aquaculture and biotechnology applications. It was previously reported to show a surprisingly low NPQd capacity while synthesizing large amounts of diatoxanthin (Dt), a pigment involved in the XC. In order to better understand this paradox, we investigated the characteristics of NPQ in T. lutea under various growth conditions of light and nutrient availability (different photoperiods, low and high light, nutrient starvations). We found a strong NPQs, unmeasurable with usual fluorometry protocols. Along with confirming the involvement of Dt in both NPQd and NPQs (by using the dithiothreitol inhibitor), we highlighted a strong relationship between Dt and the maximum quantum yield of photochemistry (Fv/Fm) across growing conditions and during relaxation experiments in darkness. It suggests that changes in Fv/Fm, usually attributed to the ‘photoinhibitory’ quenching (qI), are simultaneously largely impacted by photoprotective NPQ. The overlap of xanthophyll pigments-related photoprotective NPQ with several other mechanisms involved in the cell response (Photosystem II photoinactivation, changes in pigments composition, and detoxification by antioxidants) to energy unbalance is further discussed. Our findings question both how widespread NPQs is in the global ocean, particularly in nutrient starved environments (oligotrophic waters) and situations (post-bloom), and the use of adapted active fluorescence protocols (i.e. with extended NPQ relaxation period prior to measurement).

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Section: Xanthophyll Cycle Pigments and Their Involvement In Npqmentioning

confidence: 83%

Section: Xanthophyll Cycle Pigments and Their Involvement In Npqmentioning

confidence: 99%

Section: Xanthophyll Cycle Pigments and Their Involvement In Npqmentioning

confidence: 99%

mentioning

confidence: 99%

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Sustained xanthophyll pigments-related photoprotective NPQ is involved in photoinhibition in the haptophyte Tisochrysis lutea

Lacour,

Robert,

Lavaud

2023

Sci Rep

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“…Furthermore, the increased high light sensitivity of alka(e)ene deficient strains may also partly result from changes in the carotenoid composition. It has been shown that carotenoid content, specifically echinenone, increased sharply under high light illumination in both algae and cyanobacteria (47, 48). The sharp increase of echinenone observed in Synechocystis WT in response to high light (Fig.…”

Section: Discussionmentioning

confidence: 99%

Alka(e)nes contribute to membrane lipid homeostasis and resilience of photosynthesis to high light in cyanobacteria

Miao,

Légeret,

Cuine

et al. 2023

Preprint

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Alka(e)nes are produced by many living organisms and exhibit diverse physiological roles, reflecting a high functional versatility. Alka(e)nes serve as water proof wax in plants, communicating pheromones for insects, and microbial signaling molecules in some bacteria. Although alka(e)nes have been found in cyanobacteria and algal chloroplasts, a possible role in photosynthesis and chloroplast function remains elusive. In this study, we investigated the consequences of the absence of alka(e)nes on membrane lipid remodeling and photosynthesis using the cyanobacteriaSynechocystisPCC6803 as a model organism. By following the dynamics of membrane lipids and the photosynthetic performance in strains defected and altered in alka(e)ne biosynthesis, we show that a profound remodeling of the membrane lipidome and carotenoid content occur in the absence of alka(e)nes, including a decrease in the membrane carotenoid content, a decrease in some digalactosyldiacylglycerol (DGDG) species and a parallel increase in monogalactosyldiacylglycerol (MGDG) species. Under high light, this effect is accompanied in alka(e)ne deficient strains by a higher susceptibility of photosynthesis and growth, the effect being reversed by expressing an algal photoenzyme producing alka(e)nes from fatty acids. We conclude that alka(e)nes play a crucial role in maintaining lipid homeostasis of photosynthetic membranes, thereby contributing to the proper functioning of photosynthesis, particularly under elevated light intensities.Significance statementWe used cyanobacteria as a model organism to explore the role of alka(e)nes related to photosynthesis. Our findings reveal that the absence of alka(e)nes induces alterations in the composition of membrane lipids and carotenoid content, resulting in an increased susceptibility of photosynthesis. By introducing a fatty acid photodecarboxylase to produce alkanes, we could reverse these effects, highlighting the critical role of alka(e)nes in maintaining lipid balance in photosynthetic membranes and ensuring efficient photosynthesis. Uncovering the physiological role of alka(e)nes provides insights to a better understanding of the widespread presence of genes encoding alka(e)nes-synthesizing enzymes in cyanobacteria and microalgae, organisms of major ecological and evolutionary importance in the global CO2assimilation.

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PAR regulation of photoprotection in Phaeodactylum tricornutum (Bacillariophyceae): Roles of doses and irradiances

Cella,

Nader,

Bastolla

et al. 2023

J Appl Phycol

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Light-response in two clonal strains of the haptophyte Tisochrysis lutea: Evidence for different photoprotection strategies

Cited by 7 publications

References 58 publications

Sustained xanthophyll pigments-related photoprotective NPQ is involved in photoinhibition in the haptophyte Tisochrysis lutea

Sustained xanthophyll pigments-related photoprotective NPQ is involved in photoinhibition in the haptophyte Tisochrysis lutea

Alka(e)nes contribute to membrane lipid homeostasis and resilience of photosynthesis to high light in cyanobacteria

PAR regulation of photoprotection in Phaeodactylum tricornutum (Bacillariophyceae): Roles of doses and irradiances

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