Light response and potential interacting proteins of a grape flavonoid 3′-hydroxylase gene promoter

Sun, Ruonan; Pan, Qiu-Hong; Duan, Chang‐Qing; Wang, Jun

doi:10.1016/j.plaphy.2015.09.016

Cited by 26 publications

(22 citation statements)

References 74 publications

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“…high/low temperature, elicitors, drought, wounding, abscisic acid and ethylene) which is in accordance with the findings for e.g. the promoter regions of Vitis F3′H (Sun et al 2015) or Tulipa F3′H (Yuan et al 2014) and F3′H expression data of Sorghum bicolor (Shih et al 2006). …”

Section: Discussionsupporting

confidence: 90%

“…A varying occurrence of MdMYB10 / GFP43 gene transcripts was observed in the different plant tissues of these lines. This confirms that the isolated 1.7 kb long 5′-flanking region is sufficient to control the expression of the reporter gene as expected from sequence analysis and comparison with other F3′H promoters (Sun et al 2015; Yuan et al 2014). However, the control by the F3′H promoter was not as effective as desired as our isolated putative F3′H promoter was not active in all the lines in the organs.…”

Section: Discussionsupporting

confidence: 83%

“…Another explanation could result from the fact that the F3′H promoter, as flavonoid formation in general, could be influenced by UV (Harborne and Williams 2000; Sun et al 2015). As the plants were grown under greenhouse conditions it is possible that we did not completely exploit the potential of the F3′H promoter.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of a MdMYB10/GFP43 fusion gene for its suitability to act as reporter gene in promoter studies in Fragaria vesca L. ‘Rügen’

Khidr

Flachowsky

Haselmair-Gosch

et al. 2017

Plant Cell Tiss Organ Cult

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A Malus domestica MdMYB10 transcription factor gene was previously used as visible marker for successful plant transformation. We combined the MdMYB10 transcription factor gene with a GFP gene to test its viability as a non-destructive, visual, double reporter system for functional promoter studies in transgenic strawberry plants. The GFP gene was fused to MdMYB10 to provide evidence for promoter activity in red colored cells of transformed plant tissue and to exclude artefacts resulting from stress response or due to other environmental cues. To test this system in a first approach, we evaluated the MdMYB10-GFP43 construct in transgenic strawberries in combination with two constitutive promoters of varying strength, the strong CaMV 35S promoter and a weak flavonoid 3′-hydroxylase (F3′H) promoter isolated from the ornamental plant Cosmos sulphureus. Agrobacterium tumefaciens mediated transformation of Fragaria vesca with the MdMYB10-GFP43 construct combined with the CaMV 35S or F3′H promoter sequences resulted in the regeneration of 6 and 4 transgenic lines, respectively. A complete red coloration of all plant organs was found in four out of six transgenic lines harboring the 35S-MdMYB10-GFP43 construct. Less red coloration of plant organs was found for lines transformed with the F3′H-MdMYB10-GFP43 construct. The MdMYB10 gene shows only limited suitability as a reporter gene for promoter studies in strawberries because weak promoter activity is difficult to distinguish, particularly in tissues showing a strongly colored background such as green leaves. GFP specific fluorescence signals were detectable neither in tissue strongly expressing MdMYB10 nor in green tissue of any transgenic line. The reason for this remained unclear but it can be excluded that it was due to incorrect splicing.Electronic supplementary materialThe online version of this article (doi:10.1007/s11240-017-1229-0) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 83%

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Evaluation of a MdMYB10/GFP43 fusion gene for its suitability to act as reporter gene in promoter studies in Fragaria vesca L. ‘Rügen’

Khidr

Flachowsky

Haselmair-Gosch

et al. 2017

Plant Cell Tiss Organ Cult

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“…It was also reported that a NAC protein in Arabidopsis , ANAC078, positively regulates the expression of genes related to the biosynthesis of flavonoids, subsequently leading to the accumulation of anthocyanins in response to high-light (Morishita et al, 2009). Furthermore, VviNAC29, a protein belonging to the grapevine NAC TF superfamily, was demonstrated to act as a cooperative regulator controlling the stress-responsive expression of VviF3′H in our previous study (Sun et al, 2015b). However, their roles in the negative regulation of the flavonoid synthesis-related genes have not been investigated previously, thus, the conclusion that whether they could directly or indirectly regulate the light-response of phenolic biosynthesis still needs to be further characterized.…”

Section: Resultsmentioning

confidence: 89%

“…The subsequent cDNA synthesis and qRT-PCRs were performed as described by Sun et al (2015a). Gene-specific primers used for qRT-PCR are listed in Supplementary Table S3 (Downey et al, 2003; Castellarin et al, 2006; Fujita et al, 2006; Reid et al, 2006; Bogs et al, 2007; Czemmel et al, 2009; Shimazaki et al, 2011; Azuma et al, 2012; Sun et al, 2015b, 2016). All reactions were run in triplicate, and the normalized relative expression levels of target genes were calculated by 2 -δCt (ΔCt = Ct Target – Ct Control , Ct: cycle threshold).…”

Section: Methodsmentioning

confidence: 99%

Light-induced Variation in Phenolic Compounds in Cabernet Sauvignon Grapes (Vitis vinifera L.) Involves Extensive Transcriptome Reprogramming of Biosynthetic Enzymes, Transcription Factors, and Phytohormonal Regulators

Sun

Cheng

et al. 2017

Front. Plant Sci.

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Light environments have long been known to influence grape (Vitis vinifera L.) berry development and biosynthesis of phenolic compounds, and ultimately affect wine quality. Here, the accumulation and compositional changes of hydroxycinnamic acids (HCAs) and flavonoids, as well as global gene expression were analyzed in Cabernet Sauvignon grape berries under sunlight exposure treatments at different phenological stages. Sunlight exposure did not consistently affect the accumulation of berry skin flavan-3-ol or anthocyanin among different seasons due to climatic variations, but increased HCA content significantly at véraison and harvest, and enhanced flavonol accumulation dramatically with its timing and severity degree trend. As in sunlight exposed berries, a highly significant correlation was observed between the expression of genes coding phenylalanine ammonia-lyase, 4-coumarate: CoA ligase, flavanone 3-hydroxylase and flavonol synthase family members and corresponding metabolite accumulation in the phenolic biosynthesis pathway, which may positively or negatively be regulated by MYB, bHLH, WRKY, AP2/EREBP, C2C2, NAC, and C2H2 transcription factors (TFs). Furthermore, some candidate genes required for auxin, ethylene and abscisic acid signal transductions were also identified which are probably involved in berry development and flavonoid biosynthesis in response to enhanced sunlight irradiation. Taken together, this study provides a valuable overview of the light-induced phenolic metabolism and transcriptome changes, especially the dynamic responses of TFs and signaling components of phytohormones, and contributes to the further understanding of sunlight-responsive phenolic biosynthesis regulation in grape berries.

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Influence of Phenolic Compounds and Tannins on Wine-Related Microorganisms

Dietrich¹,

Nikfardjam²

2017

Biology of Microorganisms on Grapes, in Must and in Wine

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Light response and potential interacting proteins of a grape flavonoid 3′-hydroxylase gene promoter

Cited by 26 publications

References 74 publications

Evaluation of a MdMYB10/GFP43 fusion gene for its suitability to act as reporter gene in promoter studies in Fragaria vesca L. ‘Rügen’

Evaluation of a MdMYB10/GFP43 fusion gene for its suitability to act as reporter gene in promoter studies in Fragaria vesca L. ‘Rügen’

Light-induced Variation in Phenolic Compounds in Cabernet Sauvignon Grapes (Vitis vinifera L.) Involves Extensive Transcriptome Reprogramming of Biosynthetic Enzymes, Transcription Factors, and Phytohormonal Regulators

Influence of Phenolic Compounds and Tannins on Wine-Related Microorganisms

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