Light on depresses and light off enhances the release of dopamine from the cat's retina

Hamasaki, D.I.; Trattler, William; Hajek, Anthony S.

doi:10.1016/0304-3940(86)90239-9

Cited by 33 publications

(15 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Keeping in mind that light enhances DA metabolic activity of the retinal dopaminergic cells, it is reasonable to accept the view the light-dependent increase in DA turnover in some amacrine cells should be accompanied by light-dependent release of the neurotransmitter from these cells. It has to be mentioned that our experimental protocol (except light stimulation) was more close to that of Hamasaki et al (1986), however, the stimulation procedure and the results obtained are similar to those of Kramer (1971) and Ehinger's group (Bauer et al, 1980). Evidently, further work is needed to understand the whole sequence of events taking place in the retinal dopaminergic neurons in animals exposed to light, both constant and flickering.…”

Section: Discussionsupporting

confidence: 82%

“…Working with the rabbit eye-cup preparation, Bauer et al (1980) observed that flashing light (at 2 Hz, for 5 min) gave a small increase in the in vitro release of [3H]-DA when tested 50 min after the start of the superfusion. Recently, Hamasaki et al (1986) investigated the effects of steady light on [3H]-DA release form the cat's retina in vitro. In this study, the animals were killed, their eyes enucleated, and the isolated retinas perfused under dim illumination.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Dopamine in the rabbit retina and striatum: Diurnal rhythm and effect of light stimulation

Nowak

Zurawska

1989

J. Neural Transmission

View full text Add to dashboard Cite

In rabbits, dopamine levels in the retina, but not in the caudate nucleus, showed clear diurnal rhythm, with high values seen in the light phase. Thirty min exposition of dark-adapted rabbits to day-light produced no changes in dopamine levels in the retina. In rabbits treated with alpha-methyl-p-tyrosine, the same light exposition decreased the retinal amine level by 18%, while stimulation with intensive, flickering light significantly decreased the retinal dopamine content by 36%. Experiments performed at noon and midnight, under light or dark conditions, showed the retinal dopamine levels to be very similar in groups kept either at light or dark, irrespective of the time of the day, although in animals deprived of light the amine levels were clearly lower than in those exposed to light, both at noon and midnight. Under all experimental conditions there were no significant changes in dopamine level and utilization in the caudate nucleus. The isolated and superfused retina (preloaded with [3H]-dopamine), when stimulated with flashes of white light (2 Hz, 10 min), released [3H]-radioactivity in a Ca2+-dependent manner. It is concluded that in rabbits, light enhances dopamine levels and utilization selectively in the retina, and the observed diurnal changes in the amine metabolism are dependent on the presence or absence of light, and not on the time of the day. The proposed physiological role(s) of the retinal dopaminergic mechanisms is discussed.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Dopamine in the rabbit retina and striatum: Diurnal rhythm and effect of light stimulation

Nowak

Zurawska

1989

J. Neural Transmission

View full text Add to dashboard Cite

show abstract

“…As it has been shown that the catecholaminergic neu rons in the normal retina represent mainly the conventional amacrine cells with their somata located in INL, the results obtained in this study suggest further that those TH-I cells observed in the retinal transplant were most likely catecholaminergic amacrine cells. The fact that these cells had their sornata located in the inner parts of the INL and ramified, at least in some of the cases studied, to the adja cent IPL implying that these cells interact, as demonstrated in previous studies in the mam malian retina [24-27, 29, 34, 35], with other noncatecholaminergic or All rod amacrine cells which in turn synapse with the rod bipo lar cells [36][37][38][39], and are therefore involved in the rod pathways [35,37,40].…”

Section: Discussionmentioning

confidence: 72%

Tyrosine-Hydroxylase-Immunoreactive Neurons in Retinal Transplants in the Rat

Guo¹,

Yang²,

Liang³

et al. 1992

Biological Signals

View full text Add to dashboard Cite

Embryonic rat retinae were transplanted to the brains of newborn rats, and the distribution of catecholaminergic neurons in the retinal tissue was studied 1–2 months after transplantation, using the tyrosine hydroxylase (TH) immunohistochemical method. The results showed that distinct TH-positive cells were identified in all retinal transplants examined. The somata of the majority of these TH-immunoreactive cells were located along the inner margin of the inner nuclear layer in the retinal transplants; the processes of these cells were distributed mainly in the outer portion of the inner plexiform layer. This pattern is comparable to that observed in retinae of normal and host rats, suggesting that the organization of the catecholaminergic neurons in the transplant is largely similar to that in the normal retina. However, a reduction of the immunoreactivity in the plexiform layers and subpopulations of TH-positive cells with somatic diameter smaller than 8 µm or larger than 18 µm was observed in most of the retinal transplants studied. This implies that the organization of the catecholaminergic system in the transplant may not be as intact as in the normal retina.

show abstract

“…This research led to studies in rodents and rabbits which further characterized the effects of light and dark adaptations and of pharmacological manipulations on catecholamine neurotransmitter synthesis, release and re-uptake in retina (for reviews, see Kamp, 1985;I;y cock, 1985). Recently Hamasaki et al (1986), using cat retinas preloaded with [ HIDA, reported that at light ON there was a sustained decrease in DA release and at light OFF there was an enhanced release of [3H]DA. Their results suggested that DA release occurs in the dark phase of a flash.…”

Section: Discussionmentioning

confidence: 99%

“…However, DA may be taken up by noradrenergic or adrenergic cells (Sharman, 1981). Cat retina has been used to study various physiological and pharmacological processes which involve the neurotransmitter DA (Kramer, 1971;Thier and Alder, 1984;Maguire and Smith, 1985;Bruinink et al, 1986;Hamasaki et al, 1986), but information concerning the endogenous concentration of DA in cat retina is lacking. In addition, the presence and concentrations of the metabolites of DA, diiydroxyphenylacetic acid (DOPAC), 3-methoxytyramine (3-MT) and homovanillic acid (HVA) in cat retina have not been determined.…”

Section: Introductionmentioning

confidence: 99%

Analysis of cat retina for dopamine, dihydroxyphenylacetic acid, 3-methoxytyramine and homovanillic acid

et al. 1989

View full text Add to dashboard Cite

Twenty retinas from 10 cats were evaluated for dopamine (DA) and its metabolites dihydroxyphenylacetic acid (DOPAC), 3-methoxytyramine (3-MT) and homovanillic acid (HVA) by high pressure liquid chromatography and electrochemical detection. Dopamine was present in all 20 retinas at a mean concentration of 3.00 +/- 0.54 ng/mg protein. Dihydroxyphenylacetic acid, 3-MT and HVA were detected in 16, 14 and 9 retinas respectively. In retinas in which these metabolites were detectable, they were present in the following mean concentrations: DOPAC, 1.07 +/- 0.21 ng/mg protein; 3-MT, 3.44 +/- 0.97 ng/mg protein and HVA, 4.54 +/- 1.05 ng/mg protein. Significantly higher concentrations of 3-MT (p = 0.0108, paired t test) and HVA (p = 0.0166, paired t test) than DOPAC were present in cat retina. Linear correlation analysis between DA and its metabolites indicated that the 3-MT and DOPAC concentrations correlated well with each other and with the amount of DA in cat retina. The concentrations of the end product metabolite, HVA, had poor correlations with the concentrations of 3-MT, DOPAC or DA. These data indicated that once DA is released in cat retina it can be metabolized to 3-MT, DOPAC and HVA.

show abstract

Light on depresses and light off enhances the release of dopamine from the cat's retina

Cited by 33 publications

References 8 publications

Dopamine in the rabbit retina and striatum: Diurnal rhythm and effect of light stimulation

Dopamine in the rabbit retina and striatum: Diurnal rhythm and effect of light stimulation

Tyrosine-Hydroxylase-Immunoreactive Neurons in Retinal Transplants in the Rat

Analysis of cat retina for dopamine, dihydroxyphenylacetic acid, 3-methoxytyramine and homovanillic acid

Contact Info

Product

Resources

About