Investigation on birefringence property in uniaxial crystal (Ca 0.28 , the natural birefringence in this crystal is (n o -n e ) = 5.89 x 10 -5 with the He-Ne laser wavelength at 632.8nm and the birefringence grads with wavelength at 532nm is 5.68 x 10 -5, which indicate the good optical homogeneity of this crystal.