Light-Induced Carbonic Anhydrase Expression in <i>Chlamydomonas reinhardtii</i>

Dionisio-Sese, Maribel L.; Fukuzawa, Hideya; Miyachi, Shigetoh

doi:10.1104/pp.94.3.1103

Cited by 73 publications

(45 citation statements)

References 29 publications

(39 reference statements)

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“…In C. ellipsoidea, even light was not required for a regulation of the CCM expression (Matsuda and Colman, 1995) but in contrast, the CCM expression in C. reinhardtii was strongly blue light-dependent (Dionisio-Sese et al, 1990). In the marine diatom, P. tricornutum, also the similar signaling role of CO 2 was reported in the regulation of the CCM expression (Matsuda et al, 2001).…”

Section: Discussionmentioning

confidence: 71%

Light and CO₂/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

et al. 2015

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Our previous study showed that three CO 2 /cAMP-responsive elements (CCRE) CCRE1, CCRE2, and CCRE3 in the promoter of the chloroplastic b-carbonic anhydrase 1 gene in the marine diatom Phaeodactylum tricornutum (Pptca1) were critical for the cAMPmediated transcriptional response to ambient CO 2 concentration. Pptca1 was activated under CO 2 limitation, but the absence of light partially disabled this low-CO 2 -triggered transcriptional activation. This suppression effect disappeared when CCRE2 or two of three CCREs were replaced with a NotI restriction site, strongly suggesting that light signal cross-talks with CO 2 on the cAMP-signal transduction pathway that targets CCREs. The paralogous chloroplastic carbonic anhydrase gene, ptca2 was also CO 2 /cAMP-responsive. The upstream truncation assay of the ptca2 promoter (Pptca2) revealed a short sequence of 2367 to 2333 relative to the transcription-start site to be a critical regulatory region for the CO 2 and light responses. This core-regulatory region comprises one CCRE1 and two CCRE2 sequences. Further detailed analysis of Pptca2 clearly indicates that two CCRE2s are the cis-element governing the CO 2 /light response of Pptca2. The transcriptional activation of two Pptcas in CO 2 limitation was evident under illumination with a photosynthetically active light wavelength, and an artificial electron acceptor from the reduction side of PSI efficiently inhibited Pptcas activation, while neither inhibition of the linear electron transport from PSII to PSI nor inhibition of ATP synthesis showed an effect on the promoter activity, strongly suggesting a specific involvement of the redox level of the stromal side of the PSI in the CO 2 /light cross talk.

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Section: Discussionmentioning

confidence: 71%

Light and CO₂/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

et al. 2015

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“…An increase in the amount of TWCA1 protein present in the cells was not detected before 5 h, and steady-state levels of the protein were achieved only after 18 h. In C. reinhardtii, induction kinetics have been characterized for CAH1, a periplasmic CA. The CAH1 polypeptide can be detected 2 h after induction (Yang et al, 1985;Dionisio-Sese et al, 1990), and steady-state levels of CA are reached 4 to 6 h after induction. Similar rapid kinetics of CA induction in response to a CO 2 shift have been reported for Chlorella and Dunaliella spp.…”

Section: Discussionmentioning

confidence: 99%

Regulation of Carbonic Anhydrase Expression by Zinc, Cobalt, and Carbon Dioxide in the Marine Diatom Thalassiosira weissflogii

2000

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TWCA1 is the major Zn-requiring isoform of carbonic anhydrase (CA) in the marine diatom Thalassiosira weissflogii. We have examined the roles that trace metals and CO 2 play in the regulation of TWCA1 expression over ranges of concentrations that bracket those encountered in the marine environment. Both steady-state levels of TWCA1 and the kinetics of induction were measured by western analysis. TWCA1 levels correlated well with cellular CA activity levels. TWCA1 was induced at a low CO 2 concentration but the level of induction, as determined by western analysis, was dependent on the availability of Zn. Co effectively substituted for Zn in regulating TWCA1 expression and promoting TWCA1 activity. Upon shift from low to high CO 2 , the concentration of TWCA1 decreased. The expression of TWCA1 is diel cycle regulated, and cellular TWCA1 decreased during the dark phase. These results provide the basis for studying the expression of CA in field populations and, taken together with previous radiolabeling studies, provide strong evidence of in vivo metal substitution of Co for Zn in a CA. Our data also support the conclusion that TWCA1 plays a central role in carbon acquisition in T. weissflogii.

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“…Although originally identified as a high light-induced gene, HLA3 may in fact be responding to low CO 2 levels brought about by increased photosynthesis at higher light intensities, consistent with the induction of other CCM genes under these conditions (Im and Grossman, 2002). Similarly, CAH1 transcription requires light, low CO 2 , and photosynthetic electron flow (Dionisio-Sese et al, 1990). At the protein level, the LCIB-LCIC complex relocalizes from the stroma to the area around the pyrenoid both in response to light and to CO 2 levels (Yamano et al, 2010).…”

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confidence: 86%

Dynamics of Carbon-Concentrating Mechanism Induction and Protein Relocalization during the Dark-to-Light Transition in Synchronized Chlamydomonas reinhardtii

2014

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In the model green alga Chlamydomonas reinhardtii, a carbon-concentrating mechanism (CCM) is induced under low CO2 in the light and comprises active inorganic carbon transport components, carbonic anhydrases, and aggregation of Rubisco in the chloroplast pyrenoid. Previous studies have focused predominantly on asynchronous cultures of cells grown under low versus high CO2. Here, we have investigated the dynamics of CCM activation in synchronized cells grown in dark/light cycles compared with induction under low CO2. The specific focus was to undertake detailed time course experiments comparing physiology and gene expression during the dark-to-light transition. First, the CCM could be fully induced 1 h before dawn, as measured by the photosynthetic affinity for inorganic carbon. This occurred in advance of maximum gene transcription and protein accumulation and contrasted with the coordinated induction observed under low CO2. Between 2 and 1 h before dawn, the proportion of Rubisco and the thylakoid lumen carbonic anhydrase in the pyrenoid rose substantially, coincident with increased CCM activity. Thus, other mechanisms are likely to activate the CCM before dawn, independent of gene transcription of known CCM components. Furthermore, this study highlights the value of using synchronized cells during the dark-to-light transition as an alternative means of investigating CCM induction.

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Light-Induced Carbonic Anhydrase Expression in Chlamydomonas reinhardtii

Abstract: The effect of external inorganic carbon concentration and light on carbonic anhydrase (CA) protein accumulation and steadystate mRNA levels were examined in Chiamydomonas reinhardtii.

Cited by 73 publications

References 29 publications

Light and CO₂/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

Light and CO₂/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

Regulation of Carbonic Anhydrase Expression by Zinc, Cobalt, and Carbon Dioxide in the Marine Diatom Thalassiosira weissflogii

Dynamics of Carbon-Concentrating Mechanism Induction and Protein Relocalization during the Dark-to-Light Transition in Synchronized Chlamydomonas reinhardtii

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Light-Induced Carbonic Anhydrase Expression in Chlamydomonas reinhardtii

Abstract: The effect of external inorganic carbon concentration and light on carbonic anhydrase (CA) protein accumulation and steadystate mRNA levels were examined in Chiamydomonas reinhardtii.

Cited by 73 publications

References 29 publications

Light and CO2/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

Light and CO2/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

Regulation of Carbonic Anhydrase Expression by Zinc, Cobalt, and Carbon Dioxide in the Marine Diatom Thalassiosira weissflogii

Dynamics of Carbon-Concentrating Mechanism Induction and Protein Relocalization during the Dark-to-Light Transition in Synchronized Chlamydomonas reinhardtii

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Light and CO₂/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum

Light and CO₂/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum