Light-induced ATP driven self-assembly of actin and heavy-meromyosin in proteo-tubularsomes as a step toward artificial cells

Dhir, Shalini; Salahub, Sumalee; Mathews, Anu Stella; Kumaran, Surjith Kumar; Montemagno, Carlo; Abraham, Sinoj

doi:10.1039/c8cc02691b

Cited by 15 publications

(15 citation statements)

References 12 publications

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“…The cytoskeleton has many functions, amongst which the most important ones are cell shape and support, endocytosis, mobility and division, and thus has been gathering extensive focus in cell mimicking. 113,236,[253][254][255][256][257][258][259][260][261] In the study by Lee et al described above, the photo-switchable generation of ATP was coupled to ATP-dependent actin polymerization leading to morphological changes in the lipid GUV membrane. 236 This process was achieved by embedding magnesium ionophores in the GUV membrane and G-actin in the lumen.…”

Section: (B) Compartmentalized Processesmentioning

confidence: 99%

Liposomes and polymersomes: a comparative review towards cell mimicking

et al. 2018

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Cells are integral to all forms of life due to their compartmentalization by the plasma membrane. However, living organisms are immensely complex. Thus there is a need for simplified and controllable models of life for a deeper understanding of fundamental biological processes and man-made applications. This is where the bottom-up approach of synthetic biology comes from: a stepwise assembly of biomimetic functionalities ultimately into a protocell. A fundamental feature of such an endeavor is the generation and control of model membranes such as liposomes and polymersomes. We compare and contrast liposomes and polymersomes for a better a priori choice and design of vesicles and try to understand the advantages and shortcomings associated with using one or the other in many different aspects (properties, synthesis, self-assembly, applications) and which aspects have been studied and developed with each type and update the current development in the field.

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Section: (B) Compartmentalized Processesmentioning

confidence: 99%

Liposomes and polymersomes: a comparative review towards cell mimicking

et al. 2018

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“…Different amounts of energy can be harvested depending on where the bond cleavage occurs (Figure 2c). [41] The decomposition of ATP into ADP and AMP has key implications in the fundamental design of switchable self-assembling systems, [42][43][44][45][46][47] as it is desirable to identify systems, in which ATP can selectively induce a change, while the presence of ADP or AMP does not lead to changes. This consequently relates to changes in molecular size, amount of charges and to changes in the hydrophilic-to-hydrophobic balance and amphiphilicity of such Figure 1.…”

Section: Molecular Mechanisms For the Integration Of Atp In Self-assementioning

confidence: 99%

ATP‐Responsive and ATP‐Fueled Self‐Assembling Systems and Materials

2020

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his/her body weight in ATP each day, which is enabled through a very efficient ADP/ATP recycling system. [7] Aside of the fascinating non-equilibrium succeeded by exploiting ATP/GTP as chemical fuels to drive structures and functions, it is also important to realize that ATP and other nucleoside phosphates can be used as biological and chemical signals. [8-10] Lots of biological processes such as chromatin remodeler activation, [11] inflammatory signaling, [12] and cell differentiation [13] are mediated by ATP signaling. Additionally, cancer cells have a higher concentration of ATP, [14] which in turn provides a handle to develop new types of molecular therapies. This of course requires to develop sophisticated carriers or self-assembling structures that highly specifically react to ATP and potentially even to its concentration, ideally without any interference from other nucleoside phosphates. [15-20] For such cases, it is also important to realize that ATP is used as a chemical signal rather than a chemical fuel, as the primary objective may not be to harvest its energy from hydrolysis for functions, but just to use its chemical structure for a change of function. Overall, it becomes clear that the use of ATP (and similarly GTP) as a trigger or as a chemical fuel is of high relevance to interact with living systems and also to be able to create active devices in long term that can create work and allow for active communication in a biological environment using the fuels available therein. [21] In the scope of this review on ATP-dependent systems, and being set at the interface of near-equilibrium responsive materials versus non-equilibrium active materials, it is useful to begin Adenosine triphosphate (ATP) is a central metabolite that plays an indispensable role in various cellular processes, from energy supply to cell-tocell signaling. Nature has developed sophisticated strategies to use the energy stored in ATP for many metabolic and non-equilibrium processes, and to sense and bind ATP for biological signaling. The variations in the ATP concentrations from one organelle to another, from extracellular to intracellular environments, and from normal cells to cancer cells are one motivation for designing ATPtriggered and ATP-fueled systems and materials, because they show great potential for applications in biological systems by using ATP as a trigger or chemical fuel. Over the last decade, ATP has been emerging as an attractive co-assembling component for man-made stimuli-responsive as well as for fuel-driven active systems and materials. Herein, current advances and emerging concepts for ATP-triggered and ATP-fueled self-assemblies and materials are discussed, shedding light on applications and highlighting future developments. By bringing together concepts of different domains, that is from supramolecular chemistry to DNA nanoscience, from equilibrium to nonequilibrium self-assembly, and from fundamental sciences to applications, the aim is to cross-fertilize current approaches with the ultimate aim to bring ...

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“…[30][31][32] Moreover, in the presence of hydrophobic quinones acting as proton shuttles, the charge separated excited state of an organic molecular triad was capable of acidifying the inside of a vesicle resulting in the photocatalytic ATP formation at reconstituted ATP synthases. [33,34] Some of these light-dependent systems made further use of the generated ATP, [23,24,32] e.g. ATP synthesis was coupled to energy demanding cascade reactions such as DNA transcription.…”

Section: Introductionmentioning

confidence: 99%

TransformingEscherichia coliProteomembranes into Artificial Chloroplasts Using Molecular Photocatalysis

et al. 2022

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During the light-dependent reaction of photosynthesis, green plants couple photoinduced cascades of redox reactions with transmembrane proton translocations to generate reducing equivalents and chemical energy in the form of NADPH (nicotinamide adenine dinucleotide phosphate) and ATP (adenosine triphosphate), respectively. We mimic these basic processes by combining molecular ruthenium polypyridine-based photocatalysts and inverted vesicles derived from Escherichia coli. Upon irradiation with visible light, the interplay of photocatalytic nicotinamide reduction and enzymatic membrane-located respiration leads to the simultaneous formation of two biologically active cofactors, NADH (nicotinamide adenine dinucleotide) and ATP, respectively. This inorganic-biologic hybrid system thus emulates the cofactor delivering function of an active chloroplast.

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Light-induced ATP driven self-assembly of actin and heavy-meromyosin in proteo-tubularsomes as a step toward artificial cells

Cited by 15 publications

References 12 publications

Liposomes and polymersomes: a comparative review towards cell mimicking

Liposomes and polymersomes: a comparative review towards cell mimicking

ATP‐Responsive and ATP‐Fueled Self‐Assembling Systems and Materials

TransformingEscherichia coliProteomembranes into Artificial Chloroplasts Using Molecular Photocatalysis

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