Light‐harvesting complex gene expression is controlled by both transcriptional and post‐transcriptional mechanisms during photoacclimation in <i>Chlamydomonas reinhardtii</i>

Durnford, Dion G.; Price, Julie; McKim, Sarah M.; Sarchfield, Michelle L.

doi:10.1034/j.1399-3054.2003.00078.x

Cited by 78 publications

(75 citation statements)

References 76 publications

(102 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For C. reinhardtii cells grown photoautotrophically, a decrease in Chl content upon exposure to high light was reported before (Neale and Melis, 1986;Durnford et al, 2003;Bonente et al, 2011). Here, we show that this decrease occurs in all conditions upon exposure to high light, independent of the carbon availability.…”

Section: The Acclimation Responses To High Lightsupporting

confidence: 81%

“…The gene expression of different LHCs has been reported to be affected by light acclimation (Teramoto et al, 2002;Durnford et al, 2003;Yamano et al, 2008) and to be NAB1 regulated (Mussgnug et al, 2005). It has been observed that longterm high-light exposure of C. reinhardtii cells leads to a 50% decrease of Chl content (Neale and Melis, 1986;Bonente et al, 2012) and to changes in Chl-to-Car ratio (Niyogi et al, 1997a;Baroli et al, 2003;Bonente et al, 2012), suggesting reduction of the antenna size (Neale and Melis, 1986), although, in a more recent report , it was concluded that the antenna size is not modulated by light in this alga.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii

et al. 2016

View full text Add to dashboard Cite

Photosynthetic organisms are exposed to drastic changes in light conditions, which can affect their photosynthetic efficiency and induce photodamage. To face these changes, they have developed a series of acclimation mechanisms. In this work, we have studied the acclimation strategies of Chlamydomonas reinhardtii, a model green alga that can grow using various carbon sources and is thus an excellent system in which to study photosynthesis. Like other photosynthetic algae, it has evolved inducible mechanisms to adapt to conditions where carbon supply is limiting. We have analyzed how the carbon availability influences the composition and organization of the photosynthetic apparatus and the capacity of the cells to acclimate to different light conditions. Using electron microscopy, biochemical, and fluorescence measurements, we show that differences in CO 2 availability not only have a strong effect on the induction of the carbon-concentrating mechanisms but also change the acclimation strategy of the cells to light. For example, while cells in limiting CO 2 maintain a large antenna even in high light and switch on energy-dissipative mechanisms, cells in high CO 2 reduce the amount of pigments per cell and the antenna size. Our results show the high plasticity of the photosynthetic apparatus of C. reinhardtii. This alga is able to use various photoacclimation strategies, and the choice of which to activate strongly depends on the carbon availability.

show abstract

Section: The Acclimation Responses To High Lightsupporting

confidence: 81%

mentioning

confidence: 99%

Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Promising models propose the chloroplast redox state (for review, see Fey et al, 2005) to give rise to retrograde signals from the chloroplast to the nucleus, altering Lhc gene expression. Lhc gene transcript levels have been shown to correlate with protein levels detected (Anandan et al, 1993;Durnford et al, 2003;Ganeteg et al, 2004), but contrasting data have also been reported (Flachmann and Kü hlbrandt, 1995;Flachmann, 1997). Therefore, rising Lhc gene transcript levels alone might not necessarily lead to elevated protein abundance.…”

mentioning

confidence: 99%

Abundantly and Rarely Expressed Lhc Protein Genes Exhibit Distinct Regulation Patterns in Plants

et al. 2006

View full text Add to dashboard Cite

We have analyzed gene regulation of the Lhc supergene family in poplar (Populus spp.) and Arabidopsis (Arabidopsis thaliana) using digital expression profiling. Multivariate analysis of the tissue-specific, environmental, and developmental Lhc expression patterns in Arabidopsis and poplar was employed to characterize four rarely expressed Lhc genes, Lhca5, Lhca6, Lhcb7, and Lhcb4.3. Those genes have high expression levels under different conditions and in different tissues than the abundantly expressed Lhca1 to 4 and Lhcb1 to 6 genes that code for the 10 major types of higher plant light-harvesting proteins. However, in some of the datasets analyzed, the Lhcb4 and Lhcb6 genes as well as an Arabidopsis gene not present in poplar (Lhcb2.3) exhibited minor differences to the main cooperative Lhc gene expression pattern. The pattern of the rarely expressed Lhc genes was always found to be more similar to that of PsbS and the various light-harvesting-like genes, which might indicate distinct physiological functions for the rarely and abundantly expressed Lhc proteins. The previously undetected Lhcb7 gene encodes a novel plant Lhcb-type protein that possibly contains an additional, fourth, transmembrane N-terminal helix with a highly conserved motif. As the Lhcb4.3 gene seems to be present only in Eurosid species and as its regulation pattern varies significantly from that of Lhcb4.1 and Lhcb4.2, we conclude it to encode a distinct Lhc protein type, Lhcb8.

show abstract

“…Thus, it is not surprising that the PQ pool redox state has been identified as a key signal modulating the expression of photosynthesis-related genes in response to light conditions (8). Studies on algae like Dunaliella tertiolecta and Chlamydomonas reinhardtii (9) showed that PQ redox state influences dramatically the expression level of photosynthetic genes. In particular, the largest effects were observed in the genes encoding members of the lhc family, whose expression is strongly repressed upon exposure to strong light.…”

mentioning

confidence: 99%

Photosynthetic Antenna Size in Higher Plants Is Controlled by the Plastoquinone Redox State at the Post-transcriptional Rather than Transcriptional Level

Frigerio

Campoli²,

Zorzan

et al. 2007

Journal of Biological Chemistry

View full text Add to dashboard Cite

We analyze the effect of the plastoquinone redox state on the regulation of the light-harvesting antenna size at transcriptional and post-transcriptional levels. This was approached by studying transcription and accumulation of light-harvesting complexes in wild type versus the barley mutant viridis zb63, which is depleted in photosystem I and where plastoquinone is constitutively reduced. We show that the mRNA level of genes encoding antenna proteins is almost unaffected in the mutant; this stability of messenger level is not a peculiarity of antenna-encoding genes, but it extends to all photosynthesis-related genes. In contrast, analysis of protein accumulation by two-dimensional PAGE shows that the mutant undergoes strong reduction of its antenna size, with individual gene products having different levels of accumulation. We conclude that the plastoquinone redox state plays an important role in the long term regulation of chloroplast protein expression. However, its modulation is active at the post-transcriptional rather than transcriptional level.Sunlight is the only energy source for plants: light is absorbed by chlorophylls and carotenoids bound to the pigment-protein complexes composing photosystems I and II (PSI and -II), 2 and it is converted into chemical energy. Both photosystems are composed by two distinct moieties: (i) a core complex, responsible for charge separation and for the first steps of the electron transport, and (ii) an antenna system that increases light-harvesting capacity. In photosynthetic eukaryotes, the antenna system is composed by the members of a multigenic family called Lhc (light-harvesting complexes) (1). To avoid the over-accumulation of excitation energy in photosystems, light-absorption capacity needs to be related to the electron transport rate. To this purpose, the number of antenna proteins associated to photosystems is regulated according to the environmental conditions (2). More recently it was shown that the regulation of antenna size is restricted to PSII, whereas PSI-LHCI stoichiometry remains constant (3). Besides antenna size regulation, other mechanisms, like the state transition, the adjustment of PSI/PSII ratio, and regulation of carotenoid biosynthesis, are also involved in the plant's long term acclimation to different conditions (4). The limiting step of electron transport chain from PSII to PSI is the oxidation of plastoquinol (PQ) by cytochrome b 6 f (5); the PQ pool redox state depends on PSII excitation pressure, on the donor side, on the PSI capacity as acceptor, and on the rate of PQ reduction by cyclic electron transport (6). Thus, the ratio between reduced and oxidized plastoquinone is considered a good indicator of the balance between light absorption and electron transport rate: PQ over-reduction, in fact, suggests that electron transport is unable to use all the energy absorbed by PSII, where accumulation of excited states easily leads to the formation of harmful reactive oxygen species (ROS) (7). Thus, it is not surprising that the PQ pool redox ...

show abstract

Light‐harvesting complex gene expression is controlled by both transcriptional and post‐transcriptional mechanisms during photoacclimation in Chlamydomonas reinhardtii

Cited by 78 publications

References 76 publications

Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii

Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii

Abundantly and Rarely Expressed Lhc Protein Genes Exhibit Distinct Regulation Patterns in Plants

Photosynthetic Antenna Size in Higher Plants Is Controlled by the Plastoquinone Redox State at the Post-transcriptional Rather than Transcriptional Level

Contact Info

Product

Resources

About