Light-harvesting chlorophyll a/b-binding proteins are required for stomatal response to abscisic acid in Arabidopsis

Abstract

The light-harvesting chlorophyll a/b binding proteins (LHCB) are perhaps the most abundant membrane proteins in nature. It is reported here that the down-regulation or disruption of any member of the LHCB family, LHCB1, LHCB2, LHCB3, LHCB4, LHCB5, or LHCB6, reduces responsiveness of stomatal movement to ABA, and therefore results in a decrease in plant tolerance to drought stress in Arabidopsis thaliana. By contrast, over-expression of a LHCB member, LHCB6, enhances stomatal sensitivity to ABA. In addition, the reactive oxygen species (ROS) homeostasis and a set of ABA-responsive genes are altered in the lhcb mutants. These data demonstrate that LHCBs play a positive role in guard cell signalling in response to ABA and suggest that they may be involved in ABA signalling partly by modulating ROS homeostasis.

Stable Isotope Metabolic Labeling-based Quantitative Phosphoproteomic Analysis of Arabidopsis Mutants Reveals Ethylene-regulated Time-dependent Phosphoproteins and Putative Substrates of Constitutive Triple Response 1 Kinase

“…This observation clearly indictes that the kinase activity of CTR1 is reduced but not completely inhibited. The light-harvesting chlorophyll a/b-binding protein family is required for stomatal movement (102). Because the expression of LHCB1.1 is repressed by both glucose and fructose in wild-type Arabidopsis but not in ctr1-1 mutant, it is concluded that the sugar-mediated repression of LHCB1.1 is CTR1-dependent (103,104).…”

Comparative Transcriptomic Approaches Exploring Contamination Stress Tolerance in Salix sp. Reveal the Importance for a Metaorganismal de Novo Assembly Approach for Nonmodel Plants

“…Previous research has demonstrated that these proteins, in the antenna complexes of the photosynthetic apparatus, can be up-regulated in response to abiotic stress, perhaps due to a vulnerability to oxidative damage (Kurepin et al, 2015). In particular, light-harvesting chlorophyll a/b binding protein has been implicated as interacting with alterations to redox homeostasis via ABA signaling (Xu et al, 2012a) and in response to lead contamination (Pradeep Kumar et al, 2011). Calvin cycle CP12 (Fig.…”

Expression Profiles of <i>psbA, ALS, EPSPS</i>, and Other Chloroplastic Genes in Response to PSII-, ALS-, and EPSPS-Inhibitor Treatments in <i>Kochia scoparia</i>

“…Similarly, expression of CAB gene in soybean was reported to be downregulated at 1, 2, 4, and 8 HAT with atrazine [64]. Expression of CAB is influenced by several abiotic stress factors such as salinity, metal ions, and ultraviolet radiation [65] [66], and is known to be active during the production ROS in chloroplasts [67] [68]. …”