Light-harvesting antenna complexes in the moss Physcomitrella patens: implications for the evolutionary transition from green algae to land plants

Iwai, Masakazu; Yokono, Makio

doi:10.1016/j.pbi.2017.04.002

Cited by 23 publications

(16 citation statements)

References 59 publications

(89 reference statements)

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“…Different evolutionary groups are represented by 35 selected species (for all investigated species see Supplementary Table S3): Acam, Acorus americanus ; Agro, Agathis robusta ; Amtr, Amborella trichopoda ; Aqco, Aquilegia coerulea ; Arth, Arabidopsis; Bepe, Betula pendula ; Ceas, Centella asiatica ; Chre, Chlamydomonas reinhardtii ; Cofl, Cornus florida ; Crja, Cryptomeria japonica ; Cymi, Cycas micholitzii ; Died, Dioon edule ; Gibi, Ginkgo biloba ; Gngn, Gnetum gnemon ; Guma, Gunnera manicata ; Higr, Hibbertia grossulariifolia ; Kala, Kalanchoe laxiflora ; Lyja, Lygodium japonicum ; Mapo, Marchantia polymorpha ; Myfr, Myristica fragrans ; Noae, Nothoceros aenigmaticus ; Orsa, Oryza sativa ; Phpa, Physcomitrella patens ; Piab, P. abies ; Poru, Podocarpus rubens ; Rhto, Rhododendron tomentosum ( Ledrum palustre ); Sagl, Sarcandra glabra ; Scve, Sciadopitys verticillata ; Semo, Selaginella moellendorffii ; Soly, Solanum lycopersicum ; Spol, Spinacia oleracea ; Tacu, Taxus cuspidate ; Vivi, Vitis vinifera ; Wemi, Welwitschia mirabilis ; Xiam, Ximenia americana . ‘A’ indicates algae-specific LHCA isoforms adapted to land plant LHCA nomenclature (Alboresi et al , 2008; Iwai and Yokono, 2017); ‘M’ indicates LHCBM orthologues instead of LHCB1 and LHCB2 in Chlorophyta, Marchantiophyta, Bryophyta, and Anthocerotophyta.…”

Section: Resultsmentioning

confidence: 99%

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The unique photosynthetic apparatus of Pinaceae: analysis of photosynthetic complexes in Picea abies

Grebe

Trotta

Bajwa

et al. 2019

Journal of Experimental Botany

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Pinaceae are the predominant photosynthetic species in boreal forests, but so far no detailed description of the protein components of the photosynthetic apparatus of these gymnosperms has been available. In this study we report a detailed characterization of the thylakoid photosynthetic machinery of Norway spruce (Picea abies (L.) Karst). We first customized a spruce thylakoid protein database from translated transcript sequences combined with existing protein sequences derived from gene models, which enabled reliable tandem mass spectrometry identification of P. abies thylakoid proteins from two-dimensional large pore blue-native/SDS-PAGE. This allowed a direct comparison of the two-dimensional protein map of thylakoid protein complexes from P. abies with the model angiosperm Arabidopsis thaliana. Although the subunit composition of P. abies core PSI and PSII complexes is largely similar to that of Arabidopsis, there was a high abundance of a smaller PSI subcomplex, closely resembling the assembly intermediate PSI* complex. In addition, the evolutionary distribution of light-harvesting complex (LHC) family members of Pinaceae was compared in silico with other land plants, revealing that P. abies and other Pinaceae (also Gnetaceae and Welwitschiaceae) have lost LHCB4, but retained LHCB8 (formerly called LHCB4.3). The findings reported here show the composition of the photosynthetic apparatus of P. abies and other Pinaceae members to be unique among land plants.

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Section: Resultsmentioning

confidence: 99%

“…patens . Algal LHCA proteins were assigned to their closest land plant orthologue group (Alboresi et al , 2008; Iwai and Yokono, 2017).…”

Section: Methodsmentioning

confidence: 99%

The unique photosynthetic apparatus of Pinaceae: analysis of photosynthetic complexes in Picea abies

Grebe

Trotta

Bajwa

et al. 2019

Journal of Experimental Botany

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“…The LHC genes of the moss Physcomitrella patens are more diverse than those found in A. thaliana and C. reinhardtii and of interest to study the evolutionary transition of photosystem antenna organization [16][17][18] . Interestingly, the P. patens genome has revealed a newly identified LHCII protein, Lhcb9.…”

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confidence: 99%

A unique supramolecular organization of photosystem I in the moss Physcomitrella patens

et al. 2018

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The photosynthesis machinery in chloroplast thylakoid membranes is comprised of multiple protein complexes and supercomplexes 1,2. Here, we show a novel supramolecular organization of photosystem I (PSI) in the moss Physcomitrella patens by single-particle cryo-electron microscopy. The moss-specific light-harvesting complex (LHC) protein Lhcb9 is involved in this PSI supercomplex, which has been shown to have a molecular density similar to that of the green alga Chlamydomonas reinhardtii 3. Our results show that the structural organization is unexpectedly different-two rows of the LHCI belt exist as in C. reinhardtii 4 , but the outer one is shifted toward the PsaK side. Furthermore, one trimeric LHC protein and one monomeric LHC protein position alongside PsaL/K, filling the gap between these subunits and the outer LHCI belt. We provide evidence showing that Lhcb9 is a key factor, acting as a linkage between the PSI core and the outer LHCI belt to form the unique supramolecular organization of the PSI supercomplex in P. patens.

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“…The green algae and land plants form a monophyletic lineage named chlorophytes, the first is an ancestor of the second ( Delaux et al, 2015 ). Therefore, it is possible that they present similar primary and secondary metabolites ( Cheynier et al, 2013 ; Iwai & Yokono, 2017 ). A broad spectrum of plant extracts inhibit the influenza replication, including extracts from ethyl acetate solvent, with SI comparable to that observed in extracts 2, 4, 18, 21 and 24, from green microalgae Staurastrum sp., Scenedesmus sp., Desmodesmus armatus , Chlorellaceae and Desmidiaceae, respectively ( Hour et al, 2013 ; Wu et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effect of microalgae and cyanobacteria extracts on influenza virus replication and neuraminidase activity

Silva

Salomon

Hamerski

et al. 2018

PeerJ

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BackgroundThe influenza virus can cause seasonal infections with mild to severe symptoms, circulating worldwide, and it can affect people in any age group. Therefore, this infection is a serious public health problem that causes severe illness and death in high-risk populations. Every year, 0.5% of the world’s population is infected by this pathogen. This percentage can increase up to ten times during pandemics. Influenza vaccination is the most effective way to prevent disease. In addition, anti-influenza drugs are essential for prophylactic and therapeutic interventions. The oseltamivir (OST, a neuraminidase inhibitor) is the primary antiviral used in clinics during outbreaks. However, OST resistant viruses may emerge naturally or due to antiviral pressure, with a prevalence of 1–2% worldwide. Thus, the search for new anti-influenza drugs is extremely important. Currently, several groups have been developing studies describing the biotechnological potential of microalgae and cyanobacteria, including antiviral activity of their extracts. In Brazil, this potential is poorly known and explored.MethodsWith the aim of increasing the knowledge on this topic, 38 extracts from microalgae and cyanobacteria isolated from marine and freshwater biomes in Brazil were tested against: cellular toxicity; OST-sensitive and resistant influenza replications; and neuraminidase activity.ResultsFor this purpose, Madin-Darby Canine Kidney (MDCK)-infected cells were treated with 200 μg/mL of each extract. A total of 17 extracts (45%) inhibited influenza A replication, with seven of them resulting in more than 80% inhibition. Moreover, functional assays performed with viral neuraminidase revealed two extracts (from Leptolyngbya sp. and Chlorellaceae) with IC50 mean < 210 μg/mL for influenza A and B, and also OST-sensitive and resistant strains. Furthermore, MDCK cells exposed to 1 mg/mL of all the extracts showed viability higher than 80%.DiscussionOur results suggest that extracts of microalgae and cyanobacteria have promising anti-influenza properties. Further chemical investigation should be conducted to isolate the active compounds for the development of new anti-influenza drugs. The data generated contribute to the knowledge of the biotechnological potential of Brazilian biomes that are still little explored for this purpose.

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Light-harvesting antenna complexes in the moss Physcomitrella patens: implications for the evolutionary transition from green algae to land plants

Cited by 23 publications

References 59 publications

The unique photosynthetic apparatus of Pinaceae: analysis of photosynthetic complexes in Picea abies

The unique photosynthetic apparatus of Pinaceae: analysis of photosynthetic complexes in Picea abies

A unique supramolecular organization of photosystem I in the moss Physcomitrella patens

Inhibitory effect of microalgae and cyanobacteria extracts on influenza virus replication and neuraminidase activity

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