Light exposure of the ovum and preimplantation embryo during ART procedures

Ottosen, Lars Ditlev Mørck; Hindkjær, Johnny; Ingerslev, Hans Jakob

doi:10.1007/s10815-006-9081-x

Cited by 113 publications

(76 citation statements)

References 14 publications

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“…a typical time frame of 30 min for performing an intracytoplasmic sperm injection on the inverted microscope. It is thus important to reduce the light exposure during such in-vitro manipulations, either by decreasing exposure time and irradiance [15,27] or by avoiding harmful wavelengths by the use of filters or LED's with a more narrow spectral emission [2,28].…”

Section: Discussionmentioning

confidence: 99%

Effect of red light on the development and quality of mammalian embryos

Pedersen

Liu

et al. 2014

J Assist Reprod Genet

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Purpose To assess irradiance and total energy dose from different microscopes during the in-vitro embryonic developmental cycle in mouse and pig and to evaluate its effect on embryonic development and quality in pig. Method Spectral scalar irradiance (380-1050 nm) was measured by a fiber-optic microsensor in the focal plane of a dissection microscope, an inverted microscope and a timelapse incubation system. Furthermore, the effect of three different red light levels was tested in the time-lapse system on mouse zygotes for 5 days, and on porcine zona-intact and zona-free parthenogenetically activated (PA) embryos for 6 days. Results The time-lapse system used red light centered at 625 nm and with a lower irradiance level as compared to the white light irradiance levels on the dissection and inverted microscopes, which included more energetic radiation <550 nm. Even after 1000 times higher total energy dose of red light exposure in the time-lapse system, no significant difference was found neither in blastocyst development of mouse zygotes nor in blastocyst rates and total cell number of blastocysts of porcine PA embryos. Conclusions Our results indicate that red light (625 nm, 0.34 W/ m 2 ) used in the time-lapse incubation system does not decrease the development and quality of blastocysts in both mouse zygotes and porcine PA embryos (both zona-intact and zona-free).

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Section: Discussionmentioning

confidence: 99%

Effect of red light on the development and quality of mammalian embryos

Pedersen

Liu

et al. 2014

J Assist Reprod Genet

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“…The rabbit, whose oocytes and zygotes withstand strong light, could be an opposite extreme. Even though humans may be close to the rabbit in this respect (17), exposure of oocytes, zygotes, and *Zygotes were exposed to cool white fluorescent light (1,200 lx, 15 min) and then cultured in the dark until they reached the blastocyst stage. Zygotes handled in the same manner but shielded from light by wrapping the dishes with aluminum foil served as controls.…”

Section: Discussionmentioning

confidence: 99%

Effects of light on development of mammalian zygotes

Takenaka

Horiuchi

Yanagimachi

2007

Proc. Natl. Acad. Sci. U.S.A.

143

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It is generally assumed that light has no effect on the physiology of oocytes, zygotes, or early embryos. Therefore, little or no attention has been paid to lighting conditions during the handling of these cells in vitro. Here we show that cool white fluorescent light, rich in short-wavelength visible light and commonly used in research and clinical laboratories, produces more reactive oxygen species in mouse and hamster zygotes than does warm white fluorescent light. Mouse blastocysts that developed from zygotes shielded from light best developed to term fetuses followed by those exposed to warm white fluorescent light and then by those exposed to cool white fluorescent light. We hypothesized that light is one of the physical factors affecting embryonic environment and that its effects on cultured mammalian zygotes and embryos should not be overlooked.apoptosis ͉ fetal development ͉ hamster ͉ mouse

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“…It has been shown that extensive light exposure may be detrimental to embryo development, and especially that short wavelength light exposure should be minimized [4,27,28,40]. Furthermore, heat due to motion and friction of moving parts; presence of magnetic fields, sheer stress of moving culture dishes and presence of lubricants may each represent theoretical risks to embryo development.…”

Section: Introductionmentioning

confidence: 99%

A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

Kirkegaard

Hindkjær

Grøndahl

et al. 2012

J Assist Reprod Genet

129

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Purpose Time-lapse monitoring allows for a flexible embryo evaluation and potentially provides new dynamic markers of embryo competence. Before introducing timelapse monitoring in a clinical setting, the safety of the instrument must be properly documented. Accordingly, the aim of this study was to evaluate the safety of a commercially available time-lapse incubator. Methods In a two center, randomized, controlled, clinical trial 676 oocytes from 59 patients in their 2nd or third treatment cycle, age <38 years and ≥8 oocytes retrieved were cultured in the time-lapse incubator or in a conventional incubator. The primary outcome was proportion of 4-cell embryos on day 2. Secondary outcomes were proportion of 7-8 cell embryos on day 3 and proportion of blastocysts on day 5. Implantation pregnancy rates were registered based on presence of fetal heart activity visualized by ultrasound 8 weeks after embryo transfer. Results No significant difference was found between the time-lapse incubator (TLI) and conventional incubator (COI) in proportion of 4-cell embryos on day 2 irrespective of whether data was analyzed according to ITT (RR TLI/COI : 0.81 (0.65; 1.02)) or PP (RR TLI/COI : 0.80 (0.63; 1.01)). Nor were any significant differences detected in the secondary endpoints; i.e. proportion of 7-8-cell embryos on day three ITT (RR TLI/COI : 0.96 (0.73; 1.26)); PP (RR TLI/COI : 0.95 (0.72; 1.26)) and proportion of blastocysts on day five ITT (RR TLI/COI : 1.09 (0.84; 1.41)); PP (RR TLI/COI : 1.09 (0.83: 1.41)). We found no differences in clinical pregnancy rate or implantation rate. Conclusion Culture in the time-lapse incubator supports embryonic development equally to a conventional incubator.

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Light exposure of the ovum and preimplantation embryo during ART procedures

Cited by 113 publications

References 14 publications

Effect of red light on the development and quality of mammalian embryos

Effect of red light on the development and quality of mammalian embryos

Effects of light on development of mammalian zygotes

A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

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