Light enhanced the biosynthesis of terpenoid indole alkaloids to meet the opening of cotyledons in process of photomorphogenesis of Catharanthus roseus

Yu, Bofan; Liu, Yang; Pan, Yajie; Liu, Jia; Wang, Hongzheng; Tang, Zhonghua

doi:10.1007/s10725-017-0366-0

Cited by 15 publications

(7 citation statements)

References 47 publications

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“…Light is the basis of plant photosynthesis and plays an important role in plant growth and morphogenesis [31]. A large number of studies have shown that shading conditions can significantly change the plant height stem thickness, leaf area, leaf structure and other biological character, but also affect the change of photosynthetic efficiency, and secondary metabolites content [32,33].…”

Section: Discussionmentioning

confidence: 99%

Effects of shading on lignin biosynthesis in the leaf of tea plant (Camellia sinensis (L.) O. Kuntze)

Teng

Wang

et al. 2020

Mol Genet Genomics

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Background Lignin is one of the most important secondary metabolite that play vital functions in plant growth and development. Shading is an effective method to improve tea quality. However, little is known about the relationship between shading and xylogenesis in tea plant. Results In this study, tea plant cultivar 'Longjing 43' was treated with no shading (S0), 40% (S1) and 80% (S2) shading treatments. The leaf area and lignin content of tea plant leaves decreased under shading treatments (especially S2). The lignin distribution and anatomical characteristics of the tea plant leaves showed that lignin is mainly distributed in the xylem. Promoter analysis indicated that the genes involved in lignin pathway contain several light recognition elements. The transcript abundances of 12 lignin-associated genes were altered under shading treatments. Correlation analysis indicated that the most genes showed strong positive correlation with lignin content, and CsPAL, Cs4CL, CsF5H, and CsLAC exhibited significant positively correlation under 40% and 80% shading treatments. Conclusions In this study, we evaluated the effect of shading on leaves growth, xylem development, and lignin accumulation in tea plant. Shading treatment inhibited leaves growth and lignin content. Safranin-O/fast green staining and autofluorescence indicated lignin was mostly deposited in the xylem. Shading may affect tea plant leaves lignification by regulating the transcript levels of lignin biosynthesis genes. This study provided potentially useful information for understanding the mechanism of lignin biosynthesis under shading treatments in tea plant.

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Section: Discussionmentioning

confidence: 99%

Effects of shading on lignin biosynthesis in the leaf of tea plant (Camellia sinensis (L.) O. Kuntze)

Teng

Wang

et al. 2020

Mol Genet Genomics

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“…Third, light is a co-regulator of bis-BIAs and Chl biosynthesis in lotus plumule, and our results showed that bis-BIAs and Chl biosynthesis in lotus plumule were strongly inhibited under dark conditions, with the content of bis-BIAs and Chl decreasing by 18.4 and 75.5% at 18 DAP, respectively, relative under normal light exposure ( Figures 6B,D ). The light-induced co-regulation of alkaloids and Chl biosynthesis has previously been reported (Zhao et al, 2001 ; Zhu et al, 2015 ; Yu et al, 2018 ; Li et al, 2021 ). For example, the light improved alkaloids and Chl biosynthesis in the Catharanthus roseus callus and enhanced the content of vindoline and Chl in illuminated callus by ~ 3–4 folds and 10–20 folds, respectively (Zhao et al, 2001 ).…”

Section: Discussionmentioning

confidence: 70%

Transcriptome-Wide Characterization of Alkaloids and Chlorophyll Biosynthesis in Lotus Plumule

et al. 2022

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Lotus plumule is a green tissue in the middle of seeds that predominantly accumulates bisbenzylisoquinoline alkaloids (bis-BIAs) and chlorophyll (Chl). However, the biosynthetic mechanisms of these two metabolites remain largely unknown in lotus. This study used physiological and RNA sequencing (RNA-Seq) approaches to characterize the development and molecular mechanisms of bis-BIAs and Chl biosynthesis in lotus plumule. Physiological analysis revealed that exponential plumule growth occurred between 9 and 15 days after pollination (DAP), which coincided with the onset of bis-BIAs biosynthesis and its subsequent rapid accumulation. Transcriptome analysis of lotus plumule identified a total of 8,725 differentially expressed genes (DEGs), representing ~27.7% of all transcripts in the lotus genome. Sixteen structural DEGs, potentially associated with bis-BIAs biosynthesis, were identified. Of these, 12 encoded O-methyltransferases (OMTs) are likely involved in the methylation and bis-BIAs diversity in lotus. In addition, functionally divergent paralogous and redundant homologous gene members of the BIAs biosynthesis pathway, as well as transcription factors co-expressed with bis-BIAs and Chl biosynthesis genes, were identified. Twenty-two genes encoding 16 conserved enzymes of the Chl biosynthesis pathway were identified, with the majority being significantly upregulated by Chl biosynthesis. Photosynthesis and Chl biosynthesis pathways were simultaneously activated during lotus plumule development. Moreover, our results showed that light-driven Pchlide reduction is essential for Chl biosynthesis in the lotus plumule. These results will be useful for enhancing our understanding of alkaloids and Chl biosynthesis in plants.

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“…In the present study, flavonoid biosynthesis was, accordingly, largely enhanced only in fully opened and green cotyledons (A3) where they could act as effective UV screens. Notably, the specific accumulation of isoflavones initially occurred during the morphogenesis of A. membranaceus cotyledon In this context, terpenoid indole alkaloids were also strongly induced by light during cotyledon opening in Catharanthus roseus seedling (Yu et al 2018). Besides this, phenylalanine, the precursor of the flavonoids synthesis, also increased at A3.…”

Section: The Accumulated Flavonoids Enhanced Adaptability After Cotylmentioning

confidence: 94%

Network during light-induced cotyledons opening and greening in Astragalus membranaceus

Yang

Zhang

Liu

et al. 2020

Journal of Plant Interactions

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Opening and greening are main characteristics of morphogenesis of cotyledons. For revealing interrelationship between metabolism and morphogenesis, metabolic shifts were analyzed in cotyledon of A. membranaceus seedlings with different stages in light and in darkness. Light induced 69 metabolites (MA), related to cotyledon greening; additional 89 metabolites (MB), related to cotyledon opening, were identified by WGCNA. The screening of metabolites shared in both MA and MB obtained 37 specific metabolites (MC) related to both opening and greening. In this context, main changes in MC occurred during A3, the stage in which cotyledons fully opened and greened. Within MC, few sugars, including L-(-)-sorbose, mannose, glucose and its derivatives, markedly decreased, while other sugars, amino acids, and unsaturated fatty acids increased. Most isoflavones and flavonols including ononin, caycosin-7-glucosides, quercetin, genistein, and catechin increased 5.3, 5.5, 13.4, 6.4 and 1.8 times, respectively. Thus, accumulated flavonoids play an important role during this developmental stage.

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Light enhanced the biosynthesis of terpenoid indole alkaloids to meet the opening of cotyledons in process of photomorphogenesis of Catharanthus roseus

Cited by 15 publications

References 47 publications

Effects of shading on lignin biosynthesis in the leaf of tea plant (Camellia sinensis (L.) O. Kuntze)

Effects of shading on lignin biosynthesis in the leaf of tea plant (Camellia sinensis (L.) O. Kuntze)

Transcriptome-Wide Characterization of Alkaloids and Chlorophyll Biosynthesis in Lotus Plumule

Network during light-induced cotyledons opening and greening in Astragalus membranaceus

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