Light-emitting Diodes at 830 and 850 nm Inhibit Melanin Synthesis In vitro

Abstract

Treatment of hyperpigmentation remains a challenge. Because of the positive effects of low-energy Nd:YAG lasers on the treatment of melasma, it is suggested that laser-like light-emitting diodes (LEDs) can potentially ameliorate hyperpigmentation. We evaluated the effect of seven different LED wavelengths on melanogenesis. LED irradiation at 830 nm (dose-dependent, from 1 to 20 J/cm2) and 850 nm (1 J/cm2) significantly reduced melanin production and tyrosinase expression, not only in a normal human melanocyte monoculture both with and without forskolin stimulation but also in a three-dimensional multiple cell type culture. It reduced melanin content via inactivation of the apoptosis signal-regulating kinase and extracellular signal-regulated kinase 1/2 pathways. The level of phosphorylated cyclic AMP response element-binding protein was also decreased by LED irradiation. Moreover, LED irradiation reduced melanogenesis through decreased expression of tyrosinase family genes (tyrosinase-related protein-1 and 2, and microphthalmia-associated transcription factor). These results indicate that LEDs could potentially be used to treat melanin-overproducing skin conditions.

Biomimetic Melanosomes Promote Orientation-Selective Delivery and Melanocyte Pigmentation in the H2O2-Induced Vitiligo Mouse Model

“…Subsequently, the melanin content was determined by the sodium hydroxide lytic cell method (Figure 3c). 47 The KPV-Lipo group possesses approximately 1.48-fold greater melanin synthesis rate than that of Lipo group (P < 0.05), while no significant difference was found between free KPV, free KPV + Lipo and KPV-Lipo group. Furthermore, the expression levels of TYR, tyrosinase-related protein 1 (TRP1), and tyrosinase-related protein 2 (TRP2) were measured by Western blotting.…”

“…The KPV-Lipo and KPV+Lipo groups both showed 1.37-fold greater TYR activity than the Lipo group (Figure b). Subsequently, the melanin content was determined by the sodium hydroxide lytic cell method (Figure c) . The KPV-Lipo group possesses approximately 1.48-fold greater melanin synthesis rate than that of Lipo group ( P < 0.05), while no significant difference was found between free KPV, free KPV + Lipo and KPV-Lipo group.…”

Study on injection molding analysis of glasses-type wearable device for facial skin care

“…Studies using 630-nm and 830-nm LEDs have shown that LED treatment led to the reduction of wrinkles around the eyes and skin pigmentation and improved skin texture [ 10 , 11 , 12 ]. Kim confirmed that irradiation by 830-nm and 850-nm LEDs reduced melanin production and tyrosinase expression in normal human melanin cells [ 13 ]. Furthermore, some studies have reported that UV-induced damage was prevented when human fibroblasts were irradiated with near-infrared light [ 14 , 15 ].…”

The effects of narrowbands of visible light upon some skin disorders: a review

“…Contrary to the clinical study above, near IR light has been shown to inhibit melanin synthesis in vitro . In this in vitro study, the effect of seven different LED wavelengths upon melanogenesis was studied using cultured human melanocytes (415 nm (54.8 mW cm −2 ), 530 nm (15.8 mW cm −2 ), 630 nm (22.2 mW cm −2 ), 660 nm (17.1 mW cm −2 ), 830 nm (96 mW cm −2 ), 850 nm (114 mW cm −2 ) and 940 nm (55.5 mW cm −2 ).…”