Light-driven sodium transport in sub-bacterial particles of Halobacterium halobium

Eisenbach, Michael; Cooper, Shulamit; Garty, Haim; Johnstone, Rose M.; Rottenberg, Hagai; Caplan, S. Roy

doi:10.1016/0005-2736(77)90276-0

Cited by 77 publications

(28 citation statements)

References 28 publications

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“…halobium Ml strain was grown, as in [6], using a modified growth medium [7]. Purple membranes were isolated from the cells of the Halobacterium halobium, according to [8].…”

Section: Methodsmentioning

confidence: 99%

The low pH species of bacteriorhodopsin

Tsuji

Rosenheck

1979

FEBS Letters

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“…halobium Ml strain was grown, as in [6], using a modified growth medium [7]. Purple membranes were isolated from the cells of the Halobacterium halobium, according to [8].…”

Section: Methodsmentioning

confidence: 99%

The low pH species of bacteriorhodopsin

Tsuji

Rosenheck

1979

FEBS Letters

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“…Menadione-dependent NADH dehydrogenase activity was measured essentially as described previously [12]. Various H. volcanii membrane preparations (0.2 mg/ml) were suspended in 2 M NaCl, 0.5 mM MES, 0.5 mM PIPES, pH 6.5.…”

Section: Menadione-dependent Nadh Dehydrogenase Activitymentioning

confidence: 99%

Characterization of Inverted Membrane Vesicles from the Halophilic Archaeon Haloferax volcanii

Ring

Eichler

2001

Journal of Membrane Biology

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Abstract. Membrane-related processes in archaea, the third and most-recently described domain of life, are in general only poorly understood. One obstacle to a functional understanding of archaeal membrane-associated activities corresponds to a lack of archaeal model membrane systems. In the following, characterization of inverted archaeal membrane vesicles, prepared from the halophilic archaeon Haloferax volcanii, is presented. The inverted topology of the vesicles was revealed by defining the orientation of membrane-bound enzymes that in intact cells normally face the cytoplasm or of other protein markers, known to face the exterior medium in intact cells. Electron microscopy, protease protection assays and lectin-binding experiments confirmed the sealed nature of the vesicles. Upon alkalinization of the external medium, the vesicles were able to generate ATP, reflecting the functional nature of the membrane preparation. The availability of preparative scale amounts of inverted archaeal membrane vesicles provides a platform for the study of various membranerelated phenomena in archaea.

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“…As illustrated in Fig. 17A, the coupling between the H+/Na' antiporter [34,35,78] and the protonmotive ATPase in mitochondria1 [lo91 and bacterial [87,110] membranes provides a very interesting and educative example to conclude this commentary on coupling between purely osmotically coupled solute porter reactions and chemiosmotic protonmotive reactions. This duplex system has the important physiological function of helping to conserve the electric potential component A$ of the total protonic potential difference Ap, and increasing the effective differential pH buffering power, and the total energy storage capacity, of the osmotic system [24,34,35, 78,871 (and see [lll]).…”

Section: Coupling Between Chemiosmotic and Purely Osmotic Reactionsmentioning

confidence: 99%

Compartmentation and Communication in Living Systems. Ligand Conduction: a General Catalytic Principle in Chemical, Osmotic and Chemiosmotic Reaction Systems

Mitchell

1979

European Journal of Biochemistry

350

132

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Chemical reactions, like osmotic reactions, are transport processes when looked at in detail. Chemical catalysis by enzymes or catalytic carriers, and osmotic catalysis by porters, may be conceived as occurring by specific ligand‐conduction mechanisms. In chemiosmotic reaction systems, the pathways of specific ligand conduction are spatially orientated through anisotropic enzyme and catalytic carrier complexes in which the reactions of chemical group transfer occur as vectorial diffusion processes of group translocation down gradients of group potential that represent real spatially‐directed fields of chemical force. Thus, it is easier to explain biochemistry in terms of transport than it is to explain transport in terms of biochemistry.

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Light-driven sodium transport in sub-bacterial particles of Halobacterium halobium

Cited by 77 publications

References 28 publications

The low pH species of bacteriorhodopsin

The low pH species of bacteriorhodopsin

Characterization of Inverted Membrane Vesicles from the Halophilic Archaeon Haloferax volcanii

Compartmentation and Communication in Living Systems. Ligand Conduction: a General Catalytic Principle in Chemical, Osmotic and Chemiosmotic Reaction Systems

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