Light-dependent segregation of begomoviruses in Asystasia gangetica leaves

Wyant, Patrícia Soares; Strohmeier, Stephan; Fischer, Alexander; Schafer, Benjamin W.; Briddon, R. W.; Krenz, Björn; Jeske, Holger

doi:10.1016/j.virusres.2014.10.024

Cited by 7 publications

(4 citation statements)

References 51 publications

(53 reference statements)

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“…Sequence reads were assembled into full‐length viral sequences (c. 2.7 kb) using SeqManNGen software v.3 (DNASTAR Inc., Madison, WI) and subjected to BLASTn analysis. In addition to the ACMV DNA‐A and B components as expected, the DNA‐A and B components of Soybean chlorotic blotch virus (SbCBV) (Olufemi et al , 2010) and West African Asystasia virus 1 (WAAV1) (Wyant et al , 2015) were also identified. For SbCBV, sequence identities ranged from 97‐99% and 95‐96% for the DNA‐A and B components, respectively, whereas for WAAV1, sequence identities ranged from 92‐95% and 93‐96% for the DNA‐A and B components, respectively.…”

Section: Cameroon Site Plant Species Severity’ Of Disease Svmptomsa Acmv Sbcbv Waav1mentioning

confidence: 68%

First report of Soybean chlorotic blotch virus and West African Asystasia virus 1 infecting cassava and a wild cassava relative in Cameroon and Togo

Leke¹,

Mignouna²,

Brown³

et al. 2016

New Disease Reports

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Section: Cameroon Site Plant Species Severity’ Of Disease Svmptomsa Acmv Sbcbv Waav1mentioning

confidence: 68%

First report of Soybean chlorotic blotch virus and West African Asystasia virus 1 infecting cassava and a wild cassava relative in Cameroon and Togo

Leke¹,

Mignouna²,

Brown³

et al. 2016

New Disease Reports

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“…ToLCGdV C4 is closely related to the AC4 of Euphorbia leaf curl virus (EuLCuV), Asystasia begomovirus 2 (ABgV2), Allamanda leaf curl virus (AllLCV), and Pouzolzia mosaic Guangdong virus (PouMGDV). All these begomoviruses are firstly isolated from wild plant hosts (He et al, 2009;Tang et al, 2014;Wyant et al, 2015), demonstrating the frequent recombination between wild hosts and crops (Lefeuvre and Moriones, 2015;Garcia-Arenal and Zerbini, 2019). Recombination is a key process in the evolution of many viruses, especially for geminiviruses which contain a highly compacted genome (∼2.7 kb) (Garcia-Andres et al, 2007;Varsani et al, 2008;Martin et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

C4, the Pathogenic Determinant of Tomato Leaf Curl Guangdong Virus, May Suppress Post-transcriptional Gene Silencing by Interacting With BAM1 Protein

Tang

et al. 2020

Front. Microbiol.

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Tomato leaf curl Guangdong virus (ToLCGdV) is a begomovirus associated with a Tomato yellow leaf curl disease (TYLCD) epidemic in Guangdong province, China. Being the least conserved protein among geminivirus proteins, the function of C4 during ToLCGdV infection has not been elucidated. In this study, the infectious clones of ToLCGdV and a ToLCGdV mutant (ToLCGdV mC4 ) with disrupted C4 ORF were constructed. Although ToLCGdV and ToLCGdV mC4 could infect Nicotiana benthamiana and tomato plants, ToLCGdV mC4 elicited much milder symptoms compared with ToLCGdV. To further verify the role of C4 in viral pathogenesis, C4 was expressed in N. benthamiana from Potato virus X (PVX) vector. The results showed that ToLCGdV C4 enhanced the pathogenicity of PVX and induced more severe developmental abnormalities in plants compared with PVX alone or PVX-mC4. In addition, ToLCGdV C4 suppresses systemic gene silencing in the transgenic N. benthamiana line 16c, but not local gene silencing induced by sense GFP in wild-type N. benthamiana plants. Moreover, C4 suppresses transcriptional gene silencing (TGS) by reducing the DNA methylation level of 35S promoter in 16c-TGS N. benthamiana plants. Furthermore, C4 could also interact with the receptor-like kinase (RLK) BARELY ANY MERISTEM 1 (BAM1), suggesting that C4 may suppress gene silencing by interfering with the function of BAM1 in the cell-to-cell spread of RNAi. All these results suggest that C4 is a pathogenic determinant of ToLCGdV, and C4 may suppress post-transcriptional gene silencing (PTGS) by interacting with BAM1.

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“…Usually, limited cleavage of the RCA product with Sau 3A (or Mlu I) and insertion into Bam HI (or Eco RI) sites of the plasmids is generally applicable for all genomic components without a need to search for proper restriction enzymes. The versatile small agrobacterial plasmid pGreen [ 193 ] has been used for in planta delivery by stem inoculation or leaf infiltration with many viruses, aSats and plants [ 57 , 68 , 72 , 108 , 124 , 179 ], but other Ti-based plasmids are used routinely as well.…”

Section: Resurrection Of Virusesmentioning

confidence: 99%

Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification

Jeske

2018

Viruses

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The experience with a diagnostic technology based on rolling circle amplification (RCA), restriction fragment length polymorphism (RFLP) analyses, and direct or deep sequencing (Circomics) over the past 15 years is surveyed for the plant infecting geminiviruses, nanoviruses and associated satellite DNAs, which have had increasing impact on agricultural and horticultural losses due to global transportation and recombination-aided diversification. Current state methods for quarantine measures are described to identify individual DNA components with great accuracy and to recognize the crucial role of the molecular viral population structure as an important factor for sustainable plant protection.

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Light-dependent segregation of begomoviruses in Asystasia gangetica leaves

Cited by 7 publications

References 51 publications

First report of Soybean chlorotic blotch virus and West African Asystasia virus 1 infecting cassava and a wild cassava relative in Cameroon and Togo

First report of Soybean chlorotic blotch virus and West African Asystasia virus 1 infecting cassava and a wild cassava relative in Cameroon and Togo

C4, the Pathogenic Determinant of Tomato Leaf Curl Guangdong Virus, May Suppress Post-transcriptional Gene Silencing by Interacting With BAM1 Protein

Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification

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