Light‐dependent regulation of photosynthesis genes in <i>Rhodobacter sphaeroides</i> 2.4.1 is coordinately controlled by photosynthetic electron transport via the PrrBA two‐component system and the photoreceptor AppA

Happ, Hendrik N.; Braatsch, Stephan; Broschek, Vera; Osterloh, Lisa; Klug, Gabriele

doi:10.1111/j.1365-2958.2005.04882.x

Cited by 35 publications

(41 citation statements)

References 82 publications

(133 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most likely, energy transfer from excited bacteriochlorophyll to oxygen is enhanced when no photosynthetic electron transport can be initiated in the RC. Furthermore, we conclude that photosynthetic electron transport is not involved in light dependent regulation of phrA expression, as demonstrated for the upregulation of photosynthesis genes (Happ et al, 2005).…”

Section: Upregulation Of Phra Expression Depends On Light Intensity Amentioning

confidence: 92%

The phrA gene of Rhodobacter sphaeroides encodes a photolyase and is regulated by singlet oxygen and peroxide in a σ E-dependent manner

et al. 2007

Self Cite

View full text Add to dashboard Cite

Section: Upregulation Of Phra Expression Depends On Light Intensity Amentioning

confidence: 92%

The phrA gene of Rhodobacter sphaeroides encodes a photolyase and is regulated by singlet oxygen and peroxide in a σ E-dependent manner

et al. 2007

Self Cite

View full text Add to dashboard Cite

“…The regulation by light is initiated by the photosynthetic reaction center and transmitted by the electron transport chain and the PrrBA TCS. In addition, there is a blue-light photoreceptor, AppA, which interferes with the PrrBA-dependent regulation (95). Finally, it has been suggested that RegB in R. capsulatus may respond to additional stimuli, such as the flow of intermediates through the bacteriochlorophyll biosynthetic pathway (1).…”

Section: Regb/prrb-like Redox-responding Global Sensor Kinases With Smentioning

confidence: 99%

Stimulus Perception in Bacterial Signal-Transducing Histidine Kinases

Mascher

Helmann

Unden

2006

Microbiol Mol Biol Rev

613

652

View full text Add to dashboard Cite

SUMMARY Two-component signal-transducing systems are ubiquitously distributed communication interfaces in bacteria. They consist of a histidine kinase that senses a specific environmental stimulus and a cognate response regulator that mediates the cellular response, mostly through differential expression of target genes. Histidine kinases are typically transmembrane proteins harboring at least two domains: an input (or sensor) domain and a cytoplasmic transmitter (or kinase) domain. They can be identified and classified by virtue of their conserved cytoplasmic kinase domains. In contrast, the sensor domains are highly variable, reflecting the plethora of different signals and modes of sensing. In order to gain insight into the mechanisms of stimulus perception by bacterial histidine kinases, we here survey sensor domain architecture and topology within the bacterial membrane, functional aspects related to this topology, and sequence and phylogenetic conservation. Based on these criteria, three groups of histidine kinases can be differentiated. (i) Periplasmic-sensing histidine kinases detect their stimuli (often small solutes) through an extracellular input domain. (ii) Histidine kinases with sensing mechanisms linked to the transmembrane regions detect stimuli (usually membrane-associated stimuli, such as ionic strength, osmolarity, turgor, or functional state of the cell envelope) via their membrane-spanning segments and sometimes via additional short extracellular loops. (iii) Cytoplasmic-sensing histidine kinases (either membrane anchored or soluble) detect cellular or diffusible signals reporting the metabolic or developmental state of the cell. This review provides an overview of mechanisms of stimulus perception for members of all three groups of bacterial signal-transducing histidine kinases.

show abstract

“…However, at intermediate oxygen tension blue light, even at low intensities, prevents AppA from binding to PpsR and photosynthesis genes are repressed (8,9), reducing the accumulation of the harmful singlet oxygen. The PrrB/PrrA two-component system senses the electron transport through the cbb3 oxidase and induces transcription of photosynthesis genes at very low oxygen tension or in the absence of oxygen (5,(10)(11)(12)(13). Furthermore, the FnrL protein activates some photosynthesis genes at low oxygen tension (13) and the PpaA regulator activates some photosynthesis genes under aerobic conditions (14).…”

mentioning

confidence: 99%

Regulation of bacterial photosynthesis genes by the small noncoding RNA PcrZ

Mank

Berghoff

Hermanns

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

The small RNA PcrZ (photosynthesis control RNA Z) of the facultative phototrophic bacterium Rhodobacter sphaeroides is induced upon a drop of oxygen tension with similar kinetics to those of genes for components of photosynthetic complexes. High expression of PcrZ depends on PrrA, the response regulator of the PrrB/ PrrA two-component system with a central role in redox regulation in R. sphaeroides. In addition the FnrL protein, an activator of some photosynthesis genes at low oxygen tension, is involved in redox-dependent expression of this small (s)RNA. Overexpression of full-length PcrZ in R. sphaeroides affects expression of a small subset of genes, most of them with a function in photosynthesis. Some mRNAs from the photosynthetic gene cluster were predicted to be putative PcrZ targets and results from an in vivo reporter system support these predictions. Our data reveal a negative effect of PcrZ on expression of its target mRNAs. Thus, PcrZ counteracts the redox-dependent induction of photosynthesis genes, which is mediated by protein regulators. Because PrrA directly activates photosynthesis genes and at the same time PcrZ, which negatively affects photosynthesis gene expression, this is one of the rare cases of an incoherent feed-forward loop including an sRNA. Our data identified PcrZ as a trans acting sRNA with a direct regulatory function in formation of photosynthetic complexes and provide a model for the control of photosynthesis gene expression by a regulatory network consisting of proteins and a small noncoding RNA.α-proteobacteria | protochlorophyllide reductase

show abstract

Light‐dependent regulation of photosynthesis genes in Rhodobacter sphaeroides 2.4.1 is coordinately controlled by photosynthetic electron transport via the PrrBA two‐component system and the photoreceptor AppA

Cited by 35 publications

References 82 publications

The phrA gene of Rhodobacter sphaeroides encodes a photolyase and is regulated by singlet oxygen and peroxide in a σ E-dependent manner

The phrA gene of Rhodobacter sphaeroides encodes a photolyase and is regulated by singlet oxygen and peroxide in a σ E-dependent manner

Stimulus Perception in Bacterial Signal-Transducing Histidine Kinases

Regulation of bacterial photosynthesis genes by the small noncoding RNA PcrZ

Contact Info

Product

Resources

About