Light dependence of slow chlorophyll fluorescence induction in the course of wheat leaf ontogeny

Nesterenko, T. V.; Shikhov, V. N.; Tikhomirov, A. A.

doi:10.1134/s1607672914010116

Cited by 6 publications

(9 citation statements)

References 6 publications

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“…We showed ear lier that the ratio F v /F m , characterizing the maximal quantum efficiency of PSII photochemistry, under goes only slight (~8%) age dependent changes under normal growth conditions [12,14], whereas the ratio F p /F t (F p and F t are fluorescence intensities at the peak level P and under steady state, respectively [1]) exhib its age dependent changes with an amplitude of at least 40% [14]. Furthermore, the age dependence could be enhanced or weakened depending on the conditions of measurements [7,12,14]. Abbreviations: CFI-chlorophyll fluorescence induction; F 0 and F m -minimal and maximal levels of chlorophyll fluorescence after dark adaptation; and F t -minimal, maximal, and stationary levels of chlorophyll fluorescence in the light adapted state; F p -fluorescence intensity at the peak of CFI curve recorded under actinic illumination after preliminary dark adap tation; F v /F m -the ratio of variable (F v = F m − F 0 ) to maximal (F m ) fluorescence representing the maximal photochemical quantum yield of PSII; NPQ-non photochemical quenching in PSII; PAM-pulse amplitude modulated (fluorometry); PAR-photosynthetically active radiation; PFD-photon flux density; PSI and PSII-photosystem I and II; PSA-photosyn thetic apparatus; qN-coefficient of non photochemical quenching of chlorophyll fluorescence; qP-coefficient of pho tochemical quenching of PSII fluorescence; R fd -vitality index; Yield = − F t )/ -effective photochemical quantum yield, i.e., the fraction of light energy used by PSII complexes for electron transport.…”

Section: Introductionmentioning

confidence: 89%

“…However, the extent of this dependence for individual parameters remains poorly examined; in many cases researchers were satisfied by simple comparison of fluorescence characteristics in mature and senescent leaves using rough estimates of leaf age [7][8][9][10][11]13]. We showed ear lier that the ratio F v /F m , characterizing the maximal quantum efficiency of PSII photochemistry, under goes only slight (~8%) age dependent changes under normal growth conditions [12,14], whereas the ratio F p /F t (F p and F t are fluorescence intensities at the peak level P and under steady state, respectively [1]) exhib its age dependent changes with an amplitude of at least 40% [14]. Furthermore, the age dependence could be enhanced or weakened depending on the conditions of measurements [7,12,14].…”

Section: Introductionmentioning

confidence: 99%

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Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves

Nesterenko

Shikhov

Tikhomirov

2015

Russ J Plant Physiol

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Wheat (Triticum sativus L.) seedlings of various ages (2 to 16 day old plants) were used to study age dependent changes in the chlorophyll fluorescence induction (CFI) at various light intensities during flu orescence measurements. Plants were raised in a growth chamber using hydroponics with expanded clay, con trolled environmental conditions, and 690 µmol/(m 2 s) photon flux density (PFD) of photosynthetically active radiation (PAR). Parameters of CFI were determined under actinic PFD of 380, 580, 820, and 1340 µmol/(m 2 s) PAR. The fifth leaf from the stem base, exposed to uniform lighting, was sampled for mea surements. This leaf emerged at the plant age of 16 days. Based on fluorescence data, we calculated the max imal photochemical quantum yield of photosystem II (F v /F m ), the effective photochemical quantum yield of PSII (Yield), parameters of photochemical (qP) and non photochemical (qN and NPQ) quenching of chlo rophyll fluorescence, the F p /F t ratio, and the "vitality index" (fluorescence decrease ratio, R fd ). At moderate actinic PFD, applied commonly in PAM fluorometers (about 380 µmol/(m 2 s)), age dependent changes in NPQ, F p /F t , and R fd were observed. Analysis of CFI parameters in wheat leaves of different ages at PFD increasing from 380 to 820 µmol/(m 2 s) revealed that R fd , NPQ, and qN are the most sensitive markers of the leaf age among all parameters tested. These suitable indicators can be used for rapid assessment of the leaf age.

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Section: Introductionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves

Nesterenko

Shikhov

Tikhomirov

2015

Russ J Plant Physiol

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“…Even now, however, analysis of the literature data (Chermnykh and Kosobrukhov 1987, Loomis et al 1999, Bukhov 2004) and our previous results (Nesterenko et al 2012(Nesterenko et al , 2014 suggest that for C 3 plants, the AL intensity for measuring qN should not be higher than 400 μmol m -2 s -1 PPFD -in order to reduce the effect of the age heterogeneity of leaves. The stability of q N under intensity of actinic light between 400 and 600 μmol m -2 s -1 PPFD may be considered as an indication of leaf maturity.…”

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confidence: 94%

“…1B). It is well known that in mature leaves exposed to certain external factors, many of the parameters remain stable (Wang et al 2011, Nesterenko et al 2012, 2014, as all components of the photosynthetic apparatus of mature leaves retain their coordinated function under a wide range of irradiance levels. The high stability of photosynthesis under various lighting conditions is based on a combination of dynamic regulatory light and dark reactions of plants (Bukhov 2004).…”

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confidence: 99%

“…At the beginning of the measurements, the plants were 16 days old. Five age points corresponded to different stages of leaf development: 2-and 5-day-old leaves -young leaves; 9-day-old leaves -mature leaves; and 12-and 16-day-old leaves -aged leaves (Nesterenko et al 2014). Measurements of CFI parameters were performed with a PAM-2100 fluorometer (Heinz Walz, Germany) at irradiance levels of 380, 575, 810, and 1,330 μmol m -2 s -1 PPFD.…”

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Effect of light intensity on the age dependence of nonphotochemical fluorescence quenching in wheat leaf

Nesterenko¹,

Shikhov²,

Tikhomirov³

2015

Photosynt.

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The effects of actinic light (AL) intensity on the age dependence of nonphotochemical quenching of fluorescence (q N ) and effective quantum yield in PSII ( PSII ) were studied in continuously illuminated wheat leaves of the upper tier. Regular changes were revealed in both age dependence of q N at elevated AL intensities and light curves of q N . These changes are related to alterations in strategies of redistribution and use of absorbed light energy by the photosynthetic apparatus at different stages of wheat leaf development. Unlike Ф PSII , q N as a parameter was more sensitive to the differences in the leaf age at a certain range of light intensity. At the same time, the stability of q N at moderate light intensities may serve as an indication of leaf maturity.Additional key words: chlorophyll fluorescence induction; leaf age; photosystem II.--Nonphotochemical quenching of fluorescence (q N ) is commonly used to analyze stress tolerance in plants (Baker 2008, Ashraf and Harris 2013). It is well known that nonphotochemical quenching of chlorophyll fluorescence, q N , involves at least three molecular mechanisms: ΔpH-dependent quenching, which determines energy-dependent quenching of fluorescence (q E or the fast component, q Nf ); transition of the pigment system from state 1 to state 2 (q T or the medium component, q Nm ); and photoinactivation of PSII (q l or the slow component, q Ns ) (Baker, 2008). The contribution of each component to the total nonphotochemical quenching, q N , is determined by the actinic light intensity (Bukhov 2004), and their proportions and significance change during the leaf ontogeny (Hong et al. 1999, Müller et al. 2001, which may affect age variations in the q N as a whole. Indeed, under low and medium intensities of actinic light (AL), differences in the q N between mature and aged leaves are insignificant. These differences, however, become greater as AL intensity increases (Hong et al. 1999). Mechanisms determining nonphotochemical quenching of excited states of chlorophyll are mainly studied for PSII, as this photosystem is considered to be more sensitive to photoinhibition than PSI. Although it is well known that q N is age-and light-dependent, the age dependence of q N under medium intensities of AL and the effect of an increase in the AL intensity have not been sufficiently studied. No data have been reported on light curves of q N for leaves of different ages. The actinic light intensities reported in the literature varied between 200 and 1,500 μmol m -2 s -1 PPFD (Hong et al. 1999;Lichtenthaler et al. 2005; Wang and Chen 2011), and the available experimental data are not sufficient to evaluate the effect of the AL on the age dependence of the q N in the plant leaves. Thus, the purpose of this ---Abbreviations: CFI -chlorophyll fluorescence induction; F0 -minimal fluorescence yield of the dark-adapted state; F0' -minimal fluorescence yield of the light-adapted state; Fm -maximal fluorescence yield of the dark-adapted state; Fm' -maximal fluorescence yield of the light-ad...

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Effect of light intensity on chlorophyll fluorescence in wheat leaves: Application of PAM-fluorometry

Shikhov

Nesterenko

Tikhomirov

2016

Russ J Plant Physiol

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Light dependence of slow chlorophyll fluorescence induction in the course of wheat leaf ontogeny

Cited by 6 publications

References 6 publications

Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves

Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves

Effect of light intensity on the age dependence of nonphotochemical fluorescence quenching in wheat leaf

Effect of light intensity on chlorophyll fluorescence in wheat leaves: Application of PAM-fluorometry

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