Light controlled reversible Michael addition of cysteine: a new tool for dynamic site-specific labeling of proteins

Abstract

Cysteine-based Michael addition is a widely employed strategy for covalent conjugation of proteins, peptides, and drugs. The covalent reaction is irreversible in most cases, leading to a lack of control...

NSPs: chromogenic linkers for fast, selective, and irreversible cysteine modification

“…Recent examples include new designs for disulfide rebridging electrophiles, 36–38 tunable equilibrium constants for conjugation, 28 and electrophiles that show particularly high reaction rates. 39,40 Other conjugates can be cleaved 41 or locked on demand, 36,42 or provide additional functionality for further elaboration. 43–47 High stability 48 and excellent pharmacokinetic profiles 49 have been demonstrated.…”