Light control of RTK activity: from technology development to translational research

Leopold, Anna V.; Verkhusha, Vladislav V.

doi:10.1039/d0sc03570j

Cited by 9 publications

(5 citation statements)

References 94 publications

(119 reference statements)

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“…We further investigated ex vivo mouse samples using CW and pulsed lasers operating in the second biological window and compared the results with transmittance in the first NIR region. Since phytochromes can be activated by absorbing lowintensity light in the far-red and NIR regions [29], [56], we have used a femtosecond pulsed laser in the wavelength range of 750-830 nm that is covered by the first tissue transparency window [57]. This wavelength region is important for the photoconversion of the phytochrome to the inactive state by linear absorption [6], [10], [29].…”

Section: B Transmission Measurements With Cw and Pulsed Lasersmentioning

confidence: 99%

Ultra-Short Laser Pulses Propagation Through Mouse Head Tissues: Experimental and Computational Study

Galiakhmetova

Dremin

Koviarov

et al. 2023

IEEE J. Select. Topics Quantum Electron.

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We present a prototype and verification of a multichannel laser system applicable to optogenetic research. In vivo photostimulation of neural cells expressing photoconvertible phytochromes or opsins requires enough light irradiation delivery to the brain that cannot be supported by continues-wave (CW) light sources. The use of ultra-short pulsed (USP) lasers operating in the second near-infrared region (II-NIR) and allowing nonlinear activation and deactivation of the photoactuators is a promising method that allows to increase the penetration depth and provide spatio-temporal localisation of radiation in tissues. This study aimed to investigate the efficiency of USP light propagation in the skin, skull, and brain of the mouse head, as well as to compare it with the corresponding CW radiation propagation in the 750-830 nm and 1086-1183 nm wavelength ranges. The experimental results and computer modelling demonstrate that about 10-12% of the initial laser radiation can reach the brain tissues. These results prove that under certain conditions, the USP laser radiation can reach a penetration depth with required power that will be sufficient for non-linear activation of opsins/phytochromes in the brain of living animals.

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Section: B Transmission Measurements With Cw and Pulsed Lasersmentioning

confidence: 99%

Ultra-Short Laser Pulses Propagation Through Mouse Head Tissues: Experimental and Computational Study

Galiakhmetova

Dremin

Koviarov

et al. 2023

IEEE J. Select. Topics Quantum Electron.

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“…Since phytochromes can be activated by absorbing low-intensity light in the far-red and near-infrared regions 15,16 , we have used fs pulsed laser in the wavelength range of 750-830 nm, which is covered by the first biological window 17 . Figure 4a demonstrates the transmittance spectra of biological tissues irradiated by 750-830 nm ultra-short pulsed laser.…”

Section: Transmission Measurementsmentioning

confidence: 99%

Evaluation of penetration depth of near-infrared irradiation generated by tunable ultra-short pulsed laser in ex vivo samples of mouse head

Galiakhmetova¹,

Dremin²,

Koviarov

et al. 2022

Tissue Optics and Photonics II

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“…The paramount role of tyrosine kinases in a number of physiologically relevant pathways and the attempt to bring these major regulators under light control has also been picked up by Verkhusha and co-workers, who have recently discussed the pros and cons of the ensemble of light-activated kinases in two comprehensive reviews. , The authors fused two different receptor tyrosine kinases to the photosensory module (PAS-GAF-PHY) of the bPhy from D. radiodurans . Alternatively, they extended the photosensory part by adding the coiled coil motif of the C-terminal histidine kinase.…”

Section: Optogenetic Applicationsmentioning

confidence: 99%

The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters

et al. 2021

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This review adds the bilin-binding phytochromes to the Chemical Reviews thematic issue “Optogenetics and Photopharmacology”. The work is structured into two parts. We first outline the photochemistry of the covalently bound tetrapyrrole chromophore and summarize relevant spectroscopic, kinetic, biochemical, and physiological properties of the different families of phytochromes. Based on this knowledge, we then describe the engineering of phytochromes to further improve these chromoproteins as photoswitches and review their employment in an ever-growing number of different optogenetic applications. Most applications rely on the light-controlled complex formation between the plant photoreceptor PhyB and phytochrome-interacting factors (PIFs) or C-terminal light-regulated domains with enzymatic functions present in many bacterial and algal phytochromes. Phytochrome-based optogenetic tools are currently implemented in bacteria, yeast, plants, and animals to achieve light control of a wide range of biological activities. These cover the regulation of gene expression, protein transport into cell organelles, and the recruitment of phytochrome- or PIF-tagged proteins to membranes and other cellular compartments. This compilation illustrates the intrinsic advantages of phytochromes compared to other photoreceptor classes, e.g., their bidirectional dual-wavelength control enabling instant ON and OFF regulation. In particular, the long wavelength range of absorption and fluorescence within the “transparent window” makes phytochromes attractive for complex applications requiring deep tissue penetration or dual-wavelength control in combination with blue and UV light-sensing photoreceptors. In addition to the wide variability of applications employing natural and engineered phytochromes, we also discuss recent progress in the development of bilin-based fluorescent proteins.

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Light control of RTK activity: from technology development to translational research

Abstract: Optogenetical and optochemical approaches can be used to control RTK signalling instead of growth factors, antibodies and small-molecule inhibitors.

Cited by 9 publications

References 94 publications

Ultra-Short Laser Pulses Propagation Through Mouse Head Tissues: Experimental and Computational Study

Ultra-Short Laser Pulses Propagation Through Mouse Head Tissues: Experimental and Computational Study

Evaluation of penetration depth of near-infrared irradiation generated by tunable ultra-short pulsed laser in ex vivo samples of mouse head

The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters

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