Light Condition Influences Rutin and Polyphenol Contents in Asparagus Spears in the Mother-fern Culture System during the Summer–Autumn Harvest

Abstract

We investigated the variation in rutin and total polyphenol contents in asparagus spears under different conditions of mother-fern culture. Rutin and polyphenol contents were highest at early spring harvest at all test sites. The contents gradually decreased during the harvest season. In particular, a considerable decrease was observed after the growth of mother ferns. The absorption of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical was weaker in summer. Rutin content in the spears of plants grown outdoors was generally higher than in these grown in a greenhouse, except at early spring harvest. Rutin contents decreased in the spears of plants grown under a shading net or an ultraviolet (UV) radiation-filtering film. These results suggest that cultivation conditions, especially light conditions, have a great influence on rutin and polyphenol contents. We evaluated two new methods to increase rutin content by improving light conditions. Light conditions in asparagus rows were improved by using light-reflecting sheets and net screens; however, a significant increase in rutin content was observed only when net screening was used, implying that this method is better than light reflecting sheets to increase the rutin content.

Methyl Jasmonate Treatment Enhanced Protodioscin Biosynthesis in White Asparagus (Asparagus officinalis L.) Spears

“…Protodioscin content in the sample solution was determined using HPLC equipped with an ELSD. HPLC analysis was conducted using a Shimadzu LC9A system (Shimadzu Co., Kyoto, Japan) equipped with a Model 300S ELSD (M&S Instruments Inc., Osaka, Japan) and a Waters Sunfire C18 (4.6 × 250 mm) column (Nihon Waters K. K., Tokyo, Japan), according to the modified method described by Wang et al (2003) and Maeda et al (2010). The mobile phases consisted of 0.1% trifluoroacetic acid (A) and acetonitrile (B).…”

“…Asparagus (Asparagus officinalis L.) is a popular vegetable in Japan (Kitazawa et al, 2011;Maeda et al, 2005Maeda et al, , 2010Maeda et al, , 2012. Although green asparagus is the commonly cultivated form in Japan, the consumption of fresh white asparagus has increased because of its pleasing taste.…”

Effect of Supplemental Light on the Quality of Green Asparagus Spears in Winter ‘Fusekomi’ Forcing Culture

“…The relations between light and rutin (flavonoids) have been studied well and reported that the rutin played a role as protector against ultraviolet (UV) in living plants (Kubasek et al, 1992;1998;Wade et al, 2001;Rozema et al, 2002;Ebizawa et al, 2008). In the mother-fern culture during summer to autumn harvest, by improved the light environment rutin content become significantly high (Maeda et al, 2010). Study in buckwheat showed that rutin getting higher according to the light such as UV-B specifically (Lee et al, 2001;Kreft et al, 2002;Li et al, 2010;.…”

Comparison of Plant Growth Characteristics and Biological Activities of Four Asparagus Cultivars by Cultural Method

“…최근 연구에 의하면 비가림 하우스에서 재배 된 아스파라거스 순은 폐암세포 증식 억제 효과 등이 보고되었 다 (Lee et al, 2015). 또한 아스파라거스 순은 인체 내의 콜레스 테롤을 줄이고 암 세포 활성 억제 (Chin and Garrison, 2008;Maeda et al, 2010;Shao et al, 1996;Shou et al, 2007 간 측정하여 H2O2산화를 측정하였다 (Chen and Asada, 1989).…”