“…We performed force-dependent binding affinity assays using an optical tweezers with a 1064 nm, 10 W ytterbium fiber laser (YLR-10-1064-LP, IPG Photonics, Oxford, MA) focused on the sample plane by an oil immersion objective (CFI60 Plan Apochromat Lambda 60× N.A. 1.4, Nikon Instruments, Melville, NY), as described previously ( 48 ). Multiple polarizing beam splitters, zero-order half-wave plates, beam traps, and neutral optical density filters (ThorLabs, Newton, NJ) direct, condition, and regulate the laser power at the sample plane.…”