Light can transform the secondary structure of silk protein

Tsuboi, Yasuyuki; Ikejiri, Tadao; Shiga, Shota; Yamada, Kazutoyo; Itaya, Akira

doi:10.1007/s003390100984

Cited by 67 publications

(37 citation statements)

References 1 publication

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“…This result suggested that the conformational change of SF occurred from the random coil to the b-sheet structure due to the cross-linking with EDC. The spectral changes agree well with a result reported previously [44].…”

Section: Coating With Edc Cross-linked Sfsupporting

confidence: 83%

“…SEM micrographs of theophylline tablets coated with EDC crosslinked SF. position of absorption frequency is quite sensitive to the secondary structure [44]. The vibrational transition bands of CaO stretching (1655 cm K1 , amide I), N-H de-formation and C-N stretching (1540 cm K1 , amide II), and C-N stretching and N-H deformation (1250 cm K1 , amide III) are seen in the figure.…”

Section: Coating With Edc Cross-linked Sfmentioning

confidence: 82%

“…The spectrum before the crosslinking with EDC is apparently ascribable to the random-coil structure. With the EDC cross-linking, the most obvious feature of the b-sheet structure in IR spectroscopy is the strong absorption band at 1250 cm K1 in the amide III band [44]. Formation of amide bond between carboxylic acid and amino groups of gelatin after cross-linking with EDC was reported in the literature [26].…”

Section: Coating With Edc Cross-linked Sfmentioning

confidence: 99%

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Silk fibroin as a novel coating material for controlled release of theophylline

Bayraktar

Malay

Özgarip

et al. 2005

European Journal of Pharmaceutics and Biopharmaceutics

113

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The aim of this study was to explore potential use of the silk fibroin (SF) as an aqueous coating material for theophylline tablets. We have examined the film forming and coating properties of heat-treated fibroin, SF solution having different amounts of polyethylene glycol (PEG) and 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide (EDC) cross-linked SF. Heat-treated SF material possessed a brittle structure, which resulted in poor film forming and coating properties. The optimum PEG amount in SF solution was determined as 17% (by weight) for an acceptable film forming and zero order release profile. EDC cross-linked SF has shown a very good film forming and coating property with a potential for sustaining the drug release from coated theophylline tablets. Dissolution data for coated theophylline tablets were analyzed using Ritger and Peppas equation to describe the mechanism of drug release. Drug release from the EDC coated tablets followed zero-order kinetics. Release rate constants were found to be 0.26, 0.19, 0.16% min K1 for single-coated, double coated, and triple coated tablets, respectively. These results clearly demonstrated that silk fibroin has high utility as a novel aqueous coating material for controlled release products. q

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Section: Coating With Edc Cross-linked Sfsupporting

confidence: 83%

Section: Coating With Edc Cross-linked Sfmentioning

confidence: 82%

Section: Coating With Edc Cross-linked Sfmentioning

confidence: 99%

See 1 more Smart Citation

Silk fibroin as a novel coating material for controlled release of theophylline

Bayraktar

Malay

Özgarip

et al. 2005

European Journal of Pharmaceutics and Biopharmaceutics

113

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“…Band around 1448 cm 1 is assigned as CH 3 modes of alanine. Phenol of tyrosine is at around 1160 cm 1 [6][7][8][9].…”

Section: Atrmentioning

confidence: 99%

Performance measurement of sericin-coated silks during aging

Zhang

Wyeth

2011

Sci. China Chem.

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Silks are among the most precious ancient and historic artefacts worldwide. While washing removes the natural gum from the fibres during the fabrication of most silk textiles, for a small proportion of historic silks some or perhaps all of the sericin still remains. This paper investigated the effect of sericin coating on the aging of silk fibroin by means of ATR and tensile testing. The results show that sericin can provide some extent of protection from light and heat aging. However, in high humidity environments degummed and ungummed silk aged at the same rate because of leaching of sericin. Silk degraded at faster rate and more extensively in a moist environment. ATR could give very useful information about the aging of silk. The ATR-derived crystallinity index is good at tracing the aging factor and extent of silk deterioration. Alanine and tyrosine within fibroin, as estimated by ATR spectroscopy, are very sensitive to light, but not to heat and water. The ATR absorbance intensity ratio I amide III / I amide I is very useful for deterioration evaluation of archaeological silk objects. As a high humidity resulted in the leaching of silk, it is suggested for sericin-coated silk in collections, that not only wet cleaning is harmful, but also that storage or display in a high RH environment would be detrimental. attenuated total reflectance infrared spectroscopy, archaeological silk, sericin

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“…While the H chain contains the repetitive peptide and is more hydrophobic, the L chain is non-repetitive and more hydrophilic [35]. The secondary structure of SF is the random coil and the anti-parallel β-sheet structure, which is connected to each peptide by a hydrogen bond [36]. The main crystal structure of SF is silk I and II.…”

Section: Fibroinmentioning

confidence: 99%

Silk Protein-Based Membrane for Guided Bone Regeneration

Kwon

Seok

2018

Applied Sciences

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Silk derived from the silkworm is known for its excellent biological and mechanical properties. It has been used in various fields as a biomaterial, especially in bone tissue engineering scaffolding. Recently, silk protein-based biomaterial has been used as a barrier membrane scaffolding for guided bone regeneration (GBR). GBR promotes bone regeneration in bone defect areas using special barrier membranes. GBR membranes should have biocompatibility, biodegradability, cell occlusion, the mechanical properties of space-making, and easy clinical handling. Silk-based biomaterial has excellent biologic and mechanical properties that make it a good candidate to be used as GBR membranes. Recently, various forms of silk protein-based membranes have been introduced, demonstrating excellent bone regeneration ability, including osteogenic cell proliferation and osteogenic gene expression, and promoting new bone regeneration in vivo. In this article, we introduced the characteristics of silk protein as bone tissue engineering scaffolding and the recent application of such silk material as a GBR membrane. We also suggested future studies exploring additional uses of silk-based materials as GBR membranes.

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Light can transform the secondary structure of silk protein

Cited by 67 publications

References 1 publication

Silk fibroin as a novel coating material for controlled release of theophylline

Silk fibroin as a novel coating material for controlled release of theophylline

Performance measurement of sericin-coated silks during aging

Silk Protein-Based Membrane for Guided Bone Regeneration

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