Light assisted drying (LAD) for protein stabilization: optimization of laser processing parameters

Young, Madison A.; Antczak, Andrew T.; Elliott, Gloria D.; Trammell, Susan R.

doi:10.1117/12.2250598

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…Maintaining a low RH expedited the drying process. 21 All samples in the studies consisted of 40-μL droplets containing a model protein, egg white lysozyme (Worthington Biochemical LS002933), dissolved in drying solution (DS) at a concentration of 0.5 mg∕mL. This was verified using the absorption of light at 280 nm with a microplate spectrophotometer (Bio-Tek Synergy HT).…”

Section: Light-assisted Drying Processingmentioning

confidence: 99%

Light-assisted drying for protein stabilization

et al. 2018

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A light-based processing method to create an amorphous trehalose matrix for the stabilization of proteins is discussed. This method has potential applications in the stabilization of protein-based therapeutics and diagnostics. During light-assisted drying (LAD), proteins suspended in a trehalose solution are dehydrated using near-infrared (NIR) laser light. The goal of this study was to determine processing parameters that resulted in fast processing times and low end moisture contents (EMC), while maintaining the functionality of embedded proteins. We compared the effect of changing processing wavelength, power and resulting sample temperature, and substrate material on the EMC for two NIR laser sources (1064 and 1850 nm). The 1850-nm laser resulted in the lowest EMC (0.03 ± 0.01 gH2O / gDryWeight) after 20 min of processing on glass microfiber paper. This suggests a storage temperature of 68.3°C. We also tested the functionality of a model protein, lysozyme, after LAD processing using a standard assay. LAD showed no significant effect on the functionality of lysozyme when processed at a maximum temperature of ∼44 ° C to an EMC of 0.17 ± 0.06 gH2O / gDryWeight. LAD is a promising technique for forming amorphous trehalose solids that could stabilize proteins at ambient temperatures.

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Section: Light-assisted Drying Processingmentioning

confidence: 99%

Light-assisted drying for protein stabilization

et al. 2018

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“…Moreover, many biologics that have been lyophilized must be kept below ambient temperatures to maintain their functionality during long-term storage, which still requires cold storage for the duration of the shelf life of the product. [7][8][9][10] Previous research has demonstrated that anhydrous preservation of proteins in a trehalose amorphous solid matrix may offer a viable alternative to cold storage and freeze drying. Trehalose is an attractive option as a preservation matrix for embedded biologics due to its high viscosity (which decreases the protein denaturation rate) 5 and high glass transition temperature.…”

Section: Introductionmentioning

confidence: 99%

Light-assisted drying (LAD) for anhydrous preservation of biologics: processing of samples inside glass lyophilization vials

Tsegaye

Olson²,

Yoshino³

et al. 2023

Optics and Biophotonics in Low-Resource Settings IX

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Protein-based therapeutics are used to treat or prevent a range of diseases, but a challenge for the expanded use of these products is the need for cold storage that makes distribution difficult in low-resource settings. Lyophilization is a common method used to stabilize protein-based products. However, this process remains expensive, and many freeze-dried proteins require cold-chain storage. Anhydrous preservation in an amorphous trehalose matrix has been successfully used as an alternative to lyophilization. A new processing technique called light assisted drying (LAD) has been used to successfully dry proteins in preparation for anhydrous storage. Water is selectively heated via near-infrared (1064 nm) illumination, rapidly removing water from a sample, and forming an amorphous matrix that can be stored at supra-zero temperatures. In previous work, large volume samples (0.25 ml) were successfully LAD processed on glass coverslips, but this substrate is not typically used in industry. In this study, large volume samples are LAD processed in vials that are commonly used to lyophilize vaccines. After LAD processing, the samples are stored at room temperature (20◦C) or refrigerated (4◦C) for one month. The end moisture content of samples was determined immediately after processing/storage to evaluate the effectiveness of water removal via LAD. The trehalose matrix was characterized using polarized light imaging to determine if crystallization occurred during storage, potentially damaging embedded proteins. These preliminary studies indicate that LAD can effectively stabilize large volume samples in glass lyophilization vials and demonstrates the potential use of LAD to stabilize products such as vaccines.

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Light assisted drying (LAD) for protein stabilization: optimization of laser processing parameters

Cited by 2 publications

References 24 publications

Light-assisted drying for protein stabilization

Light-assisted drying for protein stabilization

Light-assisted drying (LAD) for anhydrous preservation of biologics: processing of samples inside glass lyophilization vials

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