Light and Scanning Electron Microscopy, Energy Dispersive X-Ray Spectroscopy, and Histochemistry of<i>Eucalyptus tereticornis</i>

Brito, Priscilla S. de; Sabedotti, Carolina; Flores, Thiago Bevilacqua; Raman, Vijayasankar; Bussade, Julia Emília; Farago, Paulo Vítor; Manfron, Jane

doi:10.1017/s1431927621012514

Cited by 15 publications

(14 citation statements)

References 40 publications

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“…This chemical composition and the shapes of crystals suggest that these crystals are composed of calcium oxalate. Recently, several authors have reported the chemical composition of the crystals using energy dispersive x‐ray spectroscopy (Brito et al, 2021; D'Almeida et al, 2021; Klider et al, 2021; Raman et al, 2022). Calcium oxalate crystals were also found in O. porosa (Brustulim et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

“…Calcium oxalate crystals are frequently found in different tissues and organs of plants (Brito et al, 2021; Horner et al, 2012; Horner & Wagner, 1995; Pauzer et al, 2021). They present a great diversity of morphologies, such as prisms of different shapes, druses, styloids, and crystal sands, and are helpful in species identification (Upton et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

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Microscopic investigations of Ocotea paranaensis―A Brazilian endemic and endangered species

Gribner

Barbosa

Monteiro

et al. 2022

Microscopy Res & Technique

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This article describes the morpho-anatomies of the leaves and stems of Ocotea paranaensis Brotto, Baitello, Cervi & Santos (Lauraceae) using light and scanning electron microscopy. The main anatomical features characterizing the species are the presence of simple non-glandular trichomes in the leaves and stems, large secretory cells in the leaves, flat-convex petioles with two small lateral ribs, brachysclereids in the stem cortex and pith, and the presence of starch grains and various types of calcium oxalate crystals in the pith. Histochemical tests indicated the presence of lignin in stone cells, fibers, and xylem. Lipophilic contents were found in the secretory cells.Phenolic compounds were detected in the epidermis, hypodermis, phloem, and xylem. The present study's findings can contribute to the taxonomy and authentication of O. paranaensis. Research highlights• This article is the first morpho-anatomical study of Ocotea paranaensis.• Anatomy and histochemistry of the leaves and stems were studied by light microscopy, FESEM and EDS.• The findings of this study would aid in the species identification and taxonomy.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Microscopic investigations of Ocotea paranaensis―A Brazilian endemic and endangered species

Gribner

Barbosa

Monteiro

et al. 2022

Microscopy Res & Technique

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“…In these fragmented tissues, it is still possible to analyze the anatomical vegetative features, corroborate the conformity of the starting material, and note any adulterations or contamination from foreign organisms. In the genus Eucalyptus , the macromorphological characteristics of taxonomic value are the shape, color, and vein pattern of the leaves, while the important micromorphological features include the type of stomata, morphology of epicuticular waxes, presence of epidermal papillae more or less prominent, and the midrib section shape [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

Eucalyptus cinerea and E. nicholii by-Products as Source of Bioactive Compounds for Agricultural Applications

Malaspina

Papaianni

Ranesi

et al. 2022

Plants

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The cultivation of different species of Eucalyptus has recently expanded in Liguria (Italy) due to the growing demand of the North European floricultural market. Eucalyptus tree branches are cut and selected for their quality, resulting in large amounts of waste biomass to be disposed of. The aim of our study was to evaluate the phytotoxic and antimicrobial activities of essential oils (EOs) from pruning wastes of E. cinerea (EC) and E. nicholii (EN), for potential applications in agriculture. Phytochemical analyses showed eucalyptol (1,8-cineole) as the major component in both EOs, but the EO yield of EN was higher than that of EC, in agreement with a significantly higher oil gland density on EN leaves. EOs from both species showed phytotoxicity on both weeds tested, but no significant inhibition on horticultural crop seed germination, except for Raphanus sativus. The EO from EC showed the strongest antibacterial activity, while the EO from EN showed the strongest antifungal activity. Concluding, EOs from Eucalyptus pruning may be used as possible alternatives to synthetic herbicides and pesticides, acting as antimicrobial and antifungal agents, thus representing a safe strategy for crop management programs.

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“…Crystal forms were compared with published images (Franceschi & Horner, 1980;Franceschi & Nakata, 2005;Chvátal, 2007;He et al;2012;Raman et al, 2014;Silva et al, 2014;Smith, 2012;Sun et al, 2015;Uloth et al, 2015;Brito et al, 2021). The location of the crystal forms was determined by visually identifying a specific tissue by focusing through the cleared material.…”

Section: Crystal Shapes and Locationsmentioning

confidence: 99%

Calcium oxalate crystal macropattern and its usefulness in the taxonomy of Baccharis (Asteraceae)

Raeski

Heiden

Novatski

et al. 2023

Preprint

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This study provides a comprehensive account of the various types of calcium oxalate crystals found in the genus Baccharis and assesses the exceptional value of crystal macropatterns for the taxonomy of the genus. The morphotype, occurrence and chemical composition of the crystals found in the stems and leaves are studied. The 44 species included in this study were selected based on a broad phylogeny-based sampling covering seven subgenera and 31 sections. These species were chosen to represent all the main phylogenetic lineages of Baccharis; thus, the sampling also represents a comprehensive coverage concerning evolutionary significance for such a large and environmentally and economically important plant group. The samples were analyzed by light microscopy, scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS). Several morphotypes of crystals, including druses, crystal sand, styloids and prisms, were present. Based on their chemical composition, the crystals were classified as pure calcium oxalate, mixtures of oxalates and sulfates, and mixtures of oxalates, sulfates and silica. The crystal macropatterns observed in this study aid in the species identification and provide novel data for the taxonomy of Baccharis.

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Light and Scanning Electron Microscopy, Energy Dispersive X-Ray Spectroscopy, and Histochemistry ofEucalyptus tereticornis

Abstract: Abstract

Cited by 15 publications

References 40 publications

Microscopic investigations of Ocotea paranaensis―A Brazilian endemic and endangered species

Microscopic investigations of Ocotea paranaensis―A Brazilian endemic and endangered species

Eucalyptus cinerea and E. nicholii by-Products as Source of Bioactive Compounds for Agricultural Applications

Calcium oxalate crystal macropattern and its usefulness in the taxonomy of Baccharis (Asteraceae)

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