Light and Plant Growth Regulators on In Vitro Proliferation

Cavallaro, Valéria; Pellegrino, Alessandra; Muleo, Rosario; Forgione, Ivano

doi:10.3390/plants11070844

Cited by 34 publications

(41 citation statements)

References 244 publications

(485 reference statements)

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“…However, the shoot length decreased with 6-BA concentrations increasing. Hence, low and high 6-BA concentrations promote the differentiation of shoots and inhibit shoot elongation and proliferation, respectively [ 40 , 41 ] ( Table 2 ). This was also observed during the induction of A. roxburghii shoots from stem nodes [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Induction, Proliferation, Regeneration and Kinsenoside and Flavonoid Content Analysis of the Anoectochilus roxburghii (Wall.) Lindl Protocorm-like Body

Wang

Chen

Yan

et al. 2022

Plants

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Anoectochilus roxburghii (Wall.) Lindl has been used in Chinese herbal medicine for treating various ailments. However, its wild resources are endangered, and artificial cultivation of the plant is limited by the low regeneration rate of conventional propagation methods. The lack of A. roxburghii resources is detrimental to the commercial production of the plant and kinsenoside, which is unique to Anoectochilus species. To develop highly efficient methods for A. roxburghii micropropagation and find alternative resources for kinsenoside production, we created an induction, proliferation, and regeneration of PLBs (IPR-PLB) protocol for A. roxburghii. We also analyzed the kinsenoside and flavonoid contents during the induction and proliferation of PLBs. The best media of IPR-PLB for PLB induction and proliferation (secondary PLB induction and proliferation), shoot formation, and rooting medium were Murashige and Skoog (MS) + 3 mg/L 6-benzylaminopurine (6-BA) + 0.5 mg/L naphthaleneacetic acid (NAA) + 0.8 mg/L zeatin (ZT) + 0.2 mg/L 2,4-dichlorophenoxyacetic acid (2, 4-D), MS + 3 mg/L 6-BA + 0.5 mg/L NAA, and MS + 0.5 mg/L NAA, respectively. On these optimized media, the PLB induction rate was 89 ± 2.08%, secondary PLB induction rate was 120 ± 5%, secondary PLB proliferation rate was 400 ± 10% and 350 ± 10 % in terms of the quantity and biomass at approximately 1 month, shoot induction rate was 10.5 shoots/PLB mass, and root induction rate was 98%. All plantlets survived after acclimation. Darkness or weak light were essential for PLB proliferation, and light was crucial for PLB differentiation on these optimized media. The kinsenoside contents of PLBs and secondary PLBs were 10.38 ± 0.08 and 12.30 ± 0.08 mg/g fresh weight (FW), respectively. Moreover, the peak kinsenoside content during the proliferation of secondary PLBs was 34.27 ± 0.79 mg/g FW, which was slightly lower than that of the whole plant (38.68 ± 3.12 mg/g FW). Two flavonoids exhibited tissue- or temporal-specific accumulation patterns, and astragalin accumulated exclusively during the first 2 weeks of cultivation. The IPR-PLB protocol for A. roxburghii may facilitate the efficient micropropagation of A. roxburghii plants. Furthermore, the PLBs are a good alternative resource for kinsenoside production.

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Section: Discussionmentioning

confidence: 99%

Induction, Proliferation, Regeneration and Kinsenoside and Flavonoid Content Analysis of the Anoectochilus roxburghii (Wall.) Lindl Protocorm-like Body

Wang

Chen

Yan

et al. 2022

Plants

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“…CKs are other plant hormones that are known to control plant growth and development, like apical development, cell division, root elongation, stomatal behavior, and chloroplast synthesis ( Cavallaro et al, 2022 ). They are produced in the root of plants from adenine compounds.…”

Section: The Rhizospherementioning

confidence: 99%

The application of plant growth-promoting rhizobacteria in Solanum lycopersicum production in the agricultural system: a review

Adedayo

Babalola

Prigent‐Combaret

et al. 2022

PeerJ

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Food safety is a significant challenge worldwide, from plantation to cultivation, especially for perishable products such as tomatoes. New eco-friendly strategies are needed, and beneficial microorganisms might be a sustainable solution. This study demonstrates bacteria activity in the tomato plant rhizosphere. Further, it investigates the rhizobacteria’s structure, function, and diversity in soil. Rhizobacteria that promote the growth and development of tomato plants are referred to as plant growth-promoting bacteria (PGPR). They form a series of associations with plants and other organisms in the soil through a mutualistic relationship where both parties benefit from living together. It implies the antagonistic activities of the rhizobacteria to deter pathogens from invading tomato plants through their roots. Some PGPR are regarded as biological control agents that hinder the development of spoilage organisms and can act as an alternative for agricultural chemicals that may be detrimental to the health of humans, animals, and some of the beneficial microbes in the rhizosphere soil. These bacteria also help tomato plants acquire essential nutrients like potassium (K), magnesium (Mg), phosphorus (P), and nitrogen (N). Some rhizobacteria may offer a solution to low tomato production and help tackle food insecurity and farming problems. In this review, an overview of soil-inhabiting rhizobacteria focused on improving the sustainable production of Solanum lycopersicum.

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“…Artificial lighting is the only source of illumination used in plant tissue cultures. In order to promote photomorphogenic reactions and photosynthetic metabolism, the lighting should emit light from the right electromagnetic spectrum areas (Cavallaro et al, 2022).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet Light Emitting Diodes (LEDs) combined with cytokinins under tissue culture conditions

ABU-ZIADA

HAROUN²,

GAMEL³

et al. 2022

Not Bot Horti Agrobo

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The present study was carried out between 2021 and 2022 at the tissue culture lab and experimental station of the vegetable and floriculture department, faculty of agriculture, Mansoura University. Using mature seeds, Moringa oleifera is propagated on a large scale and in rapid succession by in vitro culture. Two types of cytokinin: 6-benzylaminopurin (BAP), and thidiauron (TDZ) singly at three different concentrations (0, 1, or 2 mg/L), were used, as well as light-emitting diode systems (LEDs) (white as control and violet; the combination of red and blue; 1:1). After incubation for 30 days, the obtained results showed that the survival percentage increased by the treatments with the maximum value (85%) by MS medium supplemented with 2 mg/L TDZ, under violet LEDs illumination. However, the addition of thidiazuron (TDZ) to the medium did not propagate shoot, and this treatment recorded 100% callus formation other than BAP. The maximum number of axillary shoots per explant and the number of leaves recorded in the MS medium contained 2 mg/L BAP under violet LEDs. Compared to the control values, all the used treatments generally caused surprisingly stimulating the determined antioxidant enzymes and compounds (peroxidase, polyphenol oxidase &catalase and phenols, flavonoids, anthocyanin& ascorbic acid). Concerning the phytohormone content of the hormone-free medium (control), IAA, kinetin, and zeatin increased. Meanwhile, GA3 and ABA decreased with the used treatments.

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Light and Plant Growth Regulators on In Vitro Proliferation

Cited by 34 publications

References 244 publications

Induction, Proliferation, Regeneration and Kinsenoside and Flavonoid Content Analysis of the Anoectochilus roxburghii (Wall.) Lindl Protocorm-like Body

Induction, Proliferation, Regeneration and Kinsenoside and Flavonoid Content Analysis of the Anoectochilus roxburghii (Wall.) Lindl Protocorm-like Body

The application of plant growth-promoting rhizobacteria in Solanum lycopersicum production in the agricultural system: a review

Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet Light Emitting Diodes (LEDs) combined with cytokinins under tissue culture conditions

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