Light and Electron Microscopy of Dorsal Spinocerebellar Tract Neurones in the Cat: An Intracellular Horseradish Peroxidase Study

Houchin, J; Maxwell, David; Brown, A. G.; Fyffe, Robert E.W.

doi:10.1113/expphysiol.1983.sp002761

Cited by 29 publications

(15 citation statements)

References 24 publications

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“…While some attempts have been made to measure the diameter of parent and daughter branches using light microscopy, it is still controversial whether branch points in real neurons follow precise rules, such as Rall's 3/2 power law21222324. We analyzed EM 3D-reconstructions of dendritic branch points (Fig.…”

Section: Resultsmentioning

confidence: 99%

Conserved properties of dendritic trees in four cortical interneuron subtypes

Kubota

Karube

Nomura

et al. 2011

Sci Rep

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Dendritic trees influence synaptic integration and neuronal excitability, yet appear to develop in rather arbitrary patterns. Using electron microscopy and serial reconstructions, we analyzed the dendritic trees of four morphologically distinct neocortical interneuron subtypes to reveal two underlying organizational principles common to all. First, cross-sectional areas at any given point within a dendrite were proportional to the summed length of all dendritic segments distal to that point. Consistent with this observation, total cross-sectional area was almost perfectly conserved at bifurcation points. Second, dendritic cross-sections became progressively more elliptical at more proximal, larger diameter, dendritic locations. Finally, computer simulations revealed that these conserved morphological features limit distance dependent filtering of somatic EPSPs and facilitate distribution of somatic depolarization into all dendritic compartments. Because these features were shared by all interneurons studied, they may represent common organizational principles underlying the otherwise diverse morphology of dendritic trees.

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Section: Resultsmentioning

confidence: 99%

Conserved properties of dendritic trees in four cortical interneuron subtypes

Kubota

Karube

Nomura

et al. 2011

Sci Rep

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“…2) and third order dendritic segments of two cells (cf. Rose and Richmond 1981 ;Houchin et al 1983). Table 3 shows that the segments of higher order were becoming gradually thinner.…”

Section: Dendritic Lengthmentioning

confidence: 99%

“…The dendrites had only few spines and short, club-shaped processes were occasionally seen to originate from the beads (Fig. 3) (Rose and Richmond 1981 ;Houchin et al 1983;Lagerbfick and Kellert 1985).…”

Section: Dendritic Beads and Spinesmentioning

confidence: 99%

“…1977;Berthold et al 1979;Brown and Fyffe 1981). Since intracellularly injected horseradish peroxidase (HRP) provides much better means for extensive analysis of especially the distal parts of cellular processes (Jankowska et al 1976;Rastad 1981 ;Ulfhake and Kellerth 1981 ;Houchin et al 1983), we have used this stain to re-examine the morphology of Ia interneurones selecting those with input from knee extensors quadricepts, which have been found to constitute a highly functionally homogeneous subpopulation (Jankowska and Roberts 1972a, b;Hultborn 1972). The present reports presents the data on the light microscopic appearance of perikarya and dendrites of these neurones with attention to differences between the cells belonging to the same functional population.…”

Section: Introductionmentioning

confidence: 99%

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Light microscopical study of dendrites and perikarya of interneurones mediating la reciprocal inhibition of cat lumbar alpha-motoneurones

et al. 1990

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Interneurones which mediate disynaptic inhibition from la muscle spindle afferents of the quadriceps nerve to lumbar alpha-motoneurones were stained with intracellular injection of horseradish peroxidase. Seven best stained and most satisfactorily preserved cells were selected for analysis, and the light microscopic morphology of their cell bodies and dendrites were quantitatively investigated in parasagittal sections. The perikarya were located dorsal or dorso-medial to the motoneurones; they had mean diameters of 51 x 27 microns and a mean volume of 35,820 microns 3. The cells had 3 to 7 dendrites, which were arranged asymmetrically around the parent somata. The dendrites extended mainly in the dorso-ventral direction, in which the mean tip to tip distance for each cell was 1742 microns. The dendrites had few spines and they branched almost only in bifurcations. On the average, each process divided 3.5 times and in each cell they gave rise to 14.9 branching points as well as a total combined length of more than 7000 microns. Primary dendrites had a mean length of 193 microns which was generally shorter than the lengths of the branches of higher order. A more detailed analysis of two cells revealed the mean width of primary dendrites to be 5.6 microns while that of the 5th order processes was 1.5 microns. The mean tapering of individual dendritic branches per unit length was 17%, being somewhat more pronounced for the distally located segments, while at branching points the sum of daughter processes approximately equalled the diameter of the parent process.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…Our anatomical results have shown that DSCT neurones receiving monosynaptic muscle afferent input are large cells with extensive dendritic trees oriented primarily in a rostrocaudal direction (Walmsley & Nicol, 1990; see also Randic, Miletic & Loewy, B. WALMSLEY AND M. J. NICOL 1981; Houchin, Maxwell, Fyffe & Brown, 1983 (Kuno & Miyahara, 1968;Eide, Fedina, Jansen, Lundberg & Vyklicky, 1969a, b;Walmsley, 1989). Analysis of the electrotonic properties of DSCT neurones has indicated that these single-fibre EPSPs are generated at synaptic contacts on, or close to, the soma of these cells (Walmsley, 1989).…”

Section: Introductionmentioning

confidence: 99%