Light and electron microscopy imaging unveils new aspects of the antiviral capacity of silver nanoparticles in bunyavirus-infected cells

García-Serradilla, Moisés; Risco, Cristina

doi:10.1016/j.virusres.2021.198444

Cited by 16 publications

(9 citation statements)

References 63 publications

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“…Both spherical and functionalized AgNPs have been investigated. Spherical AgNPs have been demonstrated to exert a virucidal activity against IFV-A, ADV and the Bunyamwera virus (BUNV) [ 20 , 59 , 61 ]. Free infectious viral particles were pre-incubated with AgNPs for different periods and the viral morphological structure was subsequently observed by the transmission electron microscope (TEM) and compared to the untreated control.…”

Section: Wide-spectrum Antiviral Activity Of Agnpsmentioning

confidence: 99%

Silver Nanoparticles: Review of Antiviral Properties, Mechanism of Action and Applications

et al. 2023

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New antiviral drugs and new preventive antiviral strategies are a target of intense scientific interest. Thanks to their peculiar properties, nanomaterials play an important role in this field, and, in particular, among metallic materials, silver nanoparticles were demonstrated to be effective against a wide range of viruses, in addition to having a strong antibacterial effect. Although the mechanism of antiviral action is not completely clarified, silver nanoparticles can directly act on viruses, and on their first steps of interaction with the host cell, depending on several factors, such as size, shape, functionalization and concentration. This review provides an overview of the antiviral properties of silver nanoparticles, along with their demonstrated mechanisms of action and factors mainly influencing their properties. In addition, the fields of potential application are analyzed, demonstrating the versatility of silver nanoparticles, which can be involved in several devices and applications, including biomedical applications, considering both human and animal health, environmental applications, such as air filtration and water treatment, and for food and textile industry purposes. For each application, the study level of the device is indicated, if it is either a laboratory study or a commercial product.

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Section: Wide-spectrum Antiviral Activity Of Agnpsmentioning

confidence: 99%

Silver Nanoparticles: Review of Antiviral Properties, Mechanism of Action and Applications

et al. 2023

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“…Several nanoparticulates free in the cytosol may also enter the cell nucleus [ 87 , 98 , 102 , 103 , 104 , 105 ], probably by passing through the nuclear pores [ 106 ] or by being entrapped in the reassembling nuclear envelope at the end of mitosis [ 87 , 107 ]. The presence of nanoparticles inside the nucleus is potentially harmful because the interaction of the nanomaterials with the nucleic acids and/or the nuclear factors may have unpredictable consequences on gene expression and RNA processing.…”

Section: The Functional Value Of Ultrastructural Morphology In Nanomedical Investigationsmentioning

confidence: 99%

“…Fluorescence microscopy in combination with TEM and an ion beam analysis (IBA, which allows the evaluation of the chemical elemental distribution) has allowed the detection, tracking and quantification in vivo and at a high resolution of TiO 2 nanoparticles inside human keratinocytes, thus revealing the mechanisms responsible for their cytotoxicity [ 118 ]. The combination of live cell imaging and TEM revealed the intracellular distribution dynamics and the fine relationships with cell organelles of silver nanoparticles as well as the absence of ultrastructural alterations due to their administration in the frame of a study aimed at identifying novel candidates for an antiviral therapy [ 105 ]. Combining fluorescence microscopy, live cell imaging, magnetic force microscopy and TEM, the intracellular behavior of Fe 7 C 3 @C superparamagnetic nanoparticles was elucidated and their suitability as a novel contrast agent was evaluated [ 119 ].…”

Section: Combined Electron Microscopy Techniques For Nanoparticle Trackingmentioning

confidence: 99%

Transmission Electron Microscopy as a Powerful Tool to Investigate the Interaction of Nanoparticles with Subcellular Structures

Malatesta

2021

IJMS

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Nanomedical research necessarily involves the study of the interactions between nanoparticulates and the biological environment. Transmission electron microscopy has proven to be a powerful tool in providing information about nanoparticle uptake, biodistribution and relationships with cell and tissue components, thanks to its high resolution. This article aims to overview the transmission electron microscopy techniques used to explore the impact of nanoconstructs on biological systems, highlighting the functional value of ultrastructural morphology, histochemistry and microanalysis as well as their fundamental contribution to the advancement of nanomedicine.

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“…Despite their expanding worldwide significance, there are no licensed treatments or vaccines to combat bunyaviral infections [146]. García-Serradilla and Risco [147] investigated the antiviral capacity of AgNPs in BUNVinfected Vero cells and discovered that AgNPs are effective inhibitors of BUNV infection in these cells, causing changes in the ultrastructure of BUNV spherules/ROs and significantly lowering virus titers in cell supernatants.…”

Section: Bunyamwera Orthobunyavirusmentioning

confidence: 99%

Silver Nanoparticles as Potential Antiviral Agents

et al. 2021

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Since the early 1990s, nanotechnology has led to new horizons in nanomedicine, which encompasses all spheres of science including chemistry, material science, biology, and biotechnology. Emerging viral infections are creating severe hazards to public health worldwide, recently, COVID-19 has caused mass human casualties with significant economic impacts. Interestingly, silver nanoparticles (AgNPs) exhibited the potential to destroy viruses, bacteria, and fungi using various methods. However, developing safe and effective antiviral drugs is challenging, as viruses use host cells for replication. Designing drugs that do not harm host cells while targeting viruses is complicated. In recent years, the impact of AgNPs on viruses has been evaluated. Here, we discuss the potential role of silver nanoparticles as antiviral agents. In this review, we focus on the properties of AgNPs such as their characterization methods, antiviral activity, mechanisms, applications, and toxicity.

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Light and electron microscopy imaging unveils new aspects of the antiviral capacity of silver nanoparticles in bunyavirus-infected cells

Cited by 16 publications

References 63 publications

Silver Nanoparticles: Review of Antiviral Properties, Mechanism of Action and Applications

Silver Nanoparticles: Review of Antiviral Properties, Mechanism of Action and Applications

Transmission Electron Microscopy as a Powerful Tool to Investigate the Interaction of Nanoparticles with Subcellular Structures

Silver Nanoparticles as Potential Antiviral Agents

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