Light and Electron Microscopic Studies on the Cytochrome c Adjective Reaction for the Demonstration of Acid Mucopolysaccharide

Histochemical Studies on Peroxidase With Special Reference to Its Application and Inhibition Test (Rate Assay) With Fixatives

“…As a final point of interest, peroxidase reagent, in combination with cytochrome c, successfully detected acid mucopolysaccharides such as heparin, chondroitin sulfate and hyaluronic acid in tissue sections, when benzidine, diaminobenzidine, 4-Cl-l-naphthol, and guaiacol plus MBTH (3-methyl-2-benzothiazolinone hydrazone hydrochloride) were used as hydrogen donors (Mitsui et al, 1974 Mitsui, 1976). As previously reported, the critical electrolyte concentration in this method produced nearly the same tendency with a variety of tissues.…”