Light-addressable electrode with hydrogenated amorphous silicon and low-conductive passivation layer for stimulation of cultured neurons

Suzurikawa, Jun; Takahashi, Hirokazu; Kanzaki, Ryohei; Nakao, Masayuki; Takayama, Yoichiro; Jimbo, Yasuhiko

doi:10.1063/1.2709627

Cited by 27 publications

(32 citation statements)

References 13 publications

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“…For p-type silicon, electrons, which are the minority charge carriers, generate photocurrent upon application of reversed bias. Neuronal stimulation, based on this effect, was demonstrated by several groups utilizing either single crystal, [95][96][97] ( Figure 1A and B) or hydrogenated amorphous silicon (a-Si:H) [98][99][100][101] ( Figure 1C and 1D). …”

Section: Photoconductive Siliconmentioning

confidence: 99%

“…(2001), 95,96 (D) An illustration of neuronal cells light activation using a-Si:H photoconductivity according to the scheme based on Suzurikawa et al (2007). 98 In both (C and D), passivation is required due to the sensitivity of the a-Si:H layer to aqueous environment. In all schemes, conductivity is increased in the illuminated area, (under application of a voltage bias) resulting in neuronal stimulation or recording.…”

mentioning

confidence: 99%

“…Threshold bias values for different neurons, were found to vary over a wide range (−0.4 to −1.5V), and only particular locations on putative axons were susceptible to stimulation. (2001), 95,96 (D) An illustration of neuronal cells light activation using a-Si:H photoconductivity according to the scheme based on Suzurikawa et al (2007). 98 In both (C and D), passivation is required due to the sensitivity of the a-Si:H layer to aqueous environment.…”

mentioning

confidence: 99%

“…This waterproof, low-conductivity passivation layer prevents spreading currents along the plane. 98 Rat cortical cells were cultured on a photosensitive electrode, coated with poly-Dlysine and laminin for better adhesion. Neuronal activation was achieved using 470-495 nm light with intensity of 800 mW/cm 2 , and voltage pulses of 3 V for 1 ms, as verified using calcium imaging.…”

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confidence: 99%

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Novel interfaces for light directed neuronal stimulation: advances and challenges

Bareket¹,

Hanein²

2014

IJN

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Light activation of neurons is a growing field with applications ranging from basic investigation of neuronal systems to the development of new therapeutic methods such as artificial retina. Many recent studies currently explore novel methods for optical stimulation with temporal and spatial precision. Novel materials in particular provide an opportunity to enhance contemporary approaches. Here we review recent advances towards light directed interfaces for neuronal stimulation, focusing on state-of-the-art nanoengineered devices. In particular, we highlight challenges and prospects towards improved retinal prostheses.

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Section: Photoconductive Siliconmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Novel interfaces for light directed neuronal stimulation: advances and challenges

Bareket¹,

Hanein²

2014

IJN

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“…In addition, some other unconventional applications of OEK technology have also been reported. For example, Starovoytov et al presented the possibility of light addressable electrical stimulation of cultured neurons with a similar structure to OEK device by replacing the transparent ITO electrode with a floating one [119,120]. Suzurikawa et al [34] reported the production of microscale pH gradient in OEK chips.…”

Section: Discussionmentioning

confidence: 99%

Micro and Nano Manipulation and Assembly by Optically Induced Electrokinetics

Lai

et al. 2015

Micro‐ and Nanomanipulation Tools

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IntroductionIn the past 25 years, integrated circuit (IC) and microelectromechanical systems (MEMSs) technologies have moved much beyond the microdomain, and into the submicro-, nanoscopic, and, even, the molecular scales. Today, we have the capability to fabricate, manipulate, and assemble matters at the micro-, nano-, or molecular scales. This progress into "seeing" and manipulating matters that are smaller than visible light wavelength scale is impacting a range of fields including semiconductor physics, biological studies, pharmaceutical development, and many other scientific and technological applications. A range of new methods to generate physical forces have been developed in the process. For example, mechanical forces can now be applied to micro-, nano-, and biological entities using atomic force microscope probes [1], microgrippers [2], or pipettes [3]. However, disadvantages of these approaches, including inflection of mechanical damages on objects being manipulated and their relatively low manipulation speed, make manipulation of large quantities of objects difficult. In order to address these disadvantages, noncontact or noninvasive methods for micro-, nano-, and biological manipulation have also been explored in the past two decades.Manipulation devices based on magnetic forces [4], acoustic waves [5,6], hydrodynamic flows [7], light waves [8], or electrokinetic forces [9] are among the major noninvasive technologies available today. Each of them has its own advantages and disadvantages. Methods based on magnetic fields need premodification on objects with magnetic beads which constrains their application domain. In addition, movement controlled by permanent magnets is not precise enough for many proposed applications. Also, the high resistance of the magnetic coil generates significant heat during manipulation [10]. Acoustic traps show good selectivity but cannot achieve parallel manipulation of single entities. Microfluidic devices exploiting hydrodynamic flows are suitable for cell population sorting, but have difficulties in trapping or controlling specific single objects.Electrokinetic forces generated from patterned electrodes capable of inducing the desired spatial distribution of electric field have been exploited to control

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Shedding Light on Living Cells

et al. 2014

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An overview of the optical methods available to modulate the cellular activity in cell cultures and biological tissues is presented, with a focus on the use of exogenous functional materials that absorb electromagnetic radiation and transduce it into a secondary stimulus for cell excitation, with high temporal and spatial resolution. Both organic and inorganic materials are critically evaluated, for in vitro and in vivo applications. Finally, as a direct practical application of optical-stimulation techniques, the most recent results in the realization of artificial visual implants are discussed.

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Light-addressable electrode with hydrogenated amorphous silicon and low-conductive passivation layer for stimulation of cultured neurons

Cited by 27 publications

References 13 publications

Novel interfaces for light directed neuronal stimulation: advances and challenges

Novel interfaces for light directed neuronal stimulation: advances and challenges

Micro and Nano Manipulation and Assembly by Optically Induced Electrokinetics

Shedding Light on Living Cells

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