Ligation of the left renal vein in epm1-wistar rats: functional and morphologic alterations in the kidneys, testes and suprarenal glands

Abstract

Objective: The Iigation of the left renal vein (LLVR) in man is a contraversial procedure in view of the risks of lesion to the renal parenchyma. With the objecUve of studying the morphologic and functional alterations caused by these lesions, we conducted experimental research with rats. Material and Methods: 64 male adult EPM 1-WISTAR rats were used, divided into 8 groups -4 for . LLRV and four for control. Each LLRV group and corresponding control group were sacrificed progressively on the 7th, 15th, 30th and 60th day after the initial surgery. Results: We found morphofunctional alterations only in animais that underwent LLRV in the four periods of sacrifice. The proteinuria creatinine in serum, testosterone in serum and serum corticosterone in serum showed practically no alteration in relation to the normal values for rats. Statistically significant severe histological lesions were found in the kidneys and testes of the LLRV groups. Lesions in the suprarenal glands were also presentin these groups, but no sufficient to demonstrate statistical significance Conclusion: Based on these results we can conclude that the Iigation of the left renal vein is a procedure of high risk in these animais.

Analysis of testicular‐internal spermatic vein variation and the recreation of varicocoele in a Sprague‐Dawley rat model

“…Therefore, the previously reported ligation protocols must be improved to provide a consistent animal model. To improve the model, the testicular venous drainage system must be correctly characterized and described (Baptista‐Silva et al ., ; Turner, ; Gat et al ., ).…”

Left Renal Vein Division during Open Surgical Repair for Abdominal Aortic Aneurysm May Cause Long-Term Kidney Remodeling

“…S1), as previously described. 17 Blood samples and bilateral kidneys were collected from various mice prior to the procedure as a preoperative baseline and on days 1, 3, 7, and 14 following LRVL. After laparotomy under general anesthesia, blood samples were collected from the inferior vena cava with a 27-gauge needle puncture before perfusion fixation; subsequently, bilateral kidneys were harvested.…”

“…Animal models were used for histological assessments of the kidneys after left renal vein ligation (LRVL). 13,17 Computed tomography (CT) scans clinically assess postoperative kidney morphology, including kidney volume and renal cortical thickness, which are affected by renal congestion and remodeling. 18e21 The mechanism by which renal congestion affects kidney remodeling is unclear.…”