Ligation-Based Nucleic Acid Probe Methods

Electrochemical detection study of DNA

“…Figure 5-10: The gel electrophoresis image shows the amplification results of 77 bp SARS DNA collected at various cycles during the amplification. L: 1kb DNA marker; line 1: the negative control without SARS DNA template; line 2 to 22: the PCR cycle numbers of 5,6,8,10,12,14,16,18,20,22,24,26,28,30,32,34,36,37,38,39,40, respectively. The band can only be seen after cycle 22. xxiv xxv The schematic drawing of the temperature-measuring points along the PDMS microfluidic channel.…”

“…When screening for known point mutations in the sequence amplified by PCR, amplicons can be transferred (blotted) to nitrocellulose or nylon membranes and analysed by hybridisation with a labelled oligonucleotide probe specific for the mutation. Washing the membrane at a critical temperature after hybridisation removes probes bound to sequences that were not a perfect match [14]. However, this is a timeconsuming process, as it requires multiple PCR product handling steps, thus further increases the risk of contamination.…”

“…xx Figure 4-13: Square wave voltammogram for a working cycle of a DNA Gel electrophoresis of PCR amplified real sample from lambda DNA. Lane L, kb DNA ladder; lane 1, negative control (without DNA template); lane 2-21corresponding to PCR cycles 5,8,10,12,14,16,18,20,22,24,26,28,30,32,34,36,38 The real-time PCR response curves [182]. A typical amplification of a real-time PCR reaction includes a baseline, an exponential phase, and a plateau phase.…”