Ligands for label-free detection of whole bacteria on biosensors: A review

Templier, Vincent; Roux, Agnès; Roupioz, Yoann; Livache, Thierry

doi:10.1016/j.trac.2015.10.015

Cited by 94 publications

(85 citation statements)

References 66 publications

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“…Bacterial biosensors are being developed to overcome such limitation. Although the principles are different among the different methods, most of the sensors under development require a first step aimed to retain the targeted bacterium with a ligand-coated surface [23] and a second step of identification. Lectins, such as concavalin A have become one of the selected ligands [24].…”

Section: Discussionmentioning

confidence: 99%

High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from <em>Spodoptera exigua</em> (Lepidoptera: Noctuidae)

Gasmi

Ferré

Herrero

2017

Preprint

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Abstract:Lectins are carbohydrate-interacting proteins playing a pivotal role in multiple physiological and developmental aspects of all organisms. They can specifically interact with different bacterial and viral pathogens through the carbohydrate-recognition domains (CRD). In addition, lectins are also of biotechnological interest because of their potential use as biosensor for capturing and identification of bacterial species. In this work, we have characterized the bacterial agglutination properties of three C-type lectins from the Lepidoptera Spodoptera exigua. One of these lectins, BLL2, was able to agglutinate cells from a broad range of bacterial species at an extremely low concentration, becoming a very interesting protein to be used as biosensor or other biotechnological applications involving bacterial capturing.

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Section: Discussionmentioning

confidence: 99%

High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from <em>Spodoptera exigua</em> (Lepidoptera: Noctuidae)

Gasmi

Ferré

Herrero

2017

Preprint

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“…SPR systems offer a simple means of identifying bacteria in real time, without any markers. The bacteria interact with specific ligands grafted on the chip, bringing about a local change in the refractive index in the vicinity of the surface and thus a plasmonic resonance signal [5]. Using SPR systems in imaging mode allows numerous different probes to be attached to the chip's surface, meaning that numerous pathogens can be simultaneously identified in the course of a single test.…”

Section: Application In Microbiologymentioning

confidence: 99%

Surface plasmon resonance imaging: application in microbiology

2017

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Surface plasmons can be defined as the collective oscillation of free electrons on a metallic surface. They were first observed back in the early 20th century, by the American physicist and inventor R.W. Wood, while he was studying the diffraction of light by a metallic grating. Wood observed very narrow absorption bands in the diffraction spectrum that would remain unexplained until 1941, when U. Fano demonstrated that they were associated with surface waves known today as surface plasmons. A similar phenomenon explains the colours of stained-glass windows in cathedrals, as the glass contains metallic nanoparticles with plasmon absorption bands in the visible spectrum.

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“…Cela est un avantage considérable par rapport à d'autres techniques spectroscopiques ou colorimétriques qui intrinsèquement ne peuvent fonctionner que dans un milieu « propre ». C'est cette compatibilité avec les milieux complexes qui rend l'utilisation des systèmes SPR si pertinente dans le domaine agroalimentaire, où les tests doivent s'effectuer réfraction au voisinage de la surface et donc un signal de résonance plasmonique [5]. L'utilisation de systèmes SPR en mode imagerie va de plus permettre de fixer de nombreuses sondes différentes sur la surface de la puce et offre ainsi la possibilité d'identifier simultanément de nombreux pathogènes en un seul test.…”

Section: Principe De L'imagerie Sprunclassified

Imagerie par résonance des plasmons de surface : application en microbiologie

2016

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Photoniques 81P lusieurs conditions sont à respecter pour que ce couplage puisse avoir lieu [1]. D'une part, la partie réelle de la permittivité du mé-tal à la longueur d'onde d'excitation doit être négative. C'est le cas pour l'or, l'argent ou l'aluminium dans le visible et le proche infrarouge. D'autre part, il doit y avoir égalité du vecteur d'onde de la lumière incidente et celui du plasmon de surface :

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Ligands for label-free detection of whole bacteria on biosensors: A review

Cited by 94 publications

References 66 publications

High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from <em>Spodoptera exigua</em> (Lepidoptera: Noctuidae)

High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from <em>Spodoptera exigua</em> (Lepidoptera: Noctuidae)

Surface plasmon resonance imaging: application in microbiology

Imagerie par résonance des plasmons de surface : application en microbiologie

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