Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity

Bozsóki, Zoltán; Gysel, Kira; Hansen, Simon Boje; Lironi, Damiano; Krönauer, Christina; Feng, Feng; Jong, Noor de; Vinther, Maria; Kamble, Manoj; Thygesen, Mikkel B.; Engholm, Ebbe; Kofoed, Christian; Fort, Sébastien; Sullivan, John T.; Ronson, Clive W.; Jensen, Knud J.; Blaise, Mickaël; Oldroyd, Giles; Stougaard, Jens; Andersen, Kasper R.; Radutoiu, Simona

doi:10.1126/science.abb3377

Cited by 103 publications

(98 citation statements)

References 52 publications

(79 reference statements)

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“…2). The promiscuity of S. flavescens might be related to its receptor specific to Nod factor (NF) in the surface of root cells, as these receptors are related greatly to the microsymbiont spectrum of the host legumes (Bisseling and Geurts 2020;Bozsoki et al 2020). The importance of ligand-recognizing motifs in plant receptors' interaction with microbe-associated molecular pattern (MAMP) will be explained in subsequent section.…”

Section: Specificity Vs Promiscuity Of Rhizobia and Legumesmentioning

confidence: 99%

Recent development and new insight of diversification and symbiosis specificity of legume rhizobia: mechanism and application

Chen

Wang²,

et al. 2021

J Appl Microbiol

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SummaryCurrently, symbiotic rhizobia (sl., rhizobium) refer to the soil bacteria in aand b-Proteobacteria that can induce root and/or stem nodules on some legumes and a few of nonlegumes. In the nodules, rhizobia convert the inert dinitrogen gas (N 2 ) into ammonia (NH 3 ) and supply them as nitrogen nutrient to the host plant. In general, this symbiotic association presents specificity between rhizobial and leguminous species, and most of the rhizobia use lipochitooligosaccharides, so called Nod factor (NF), for cooperating with their host plant to initiate the formation of nodule primordium and to inhibit the plant immunity. Besides NF, effectors secreted by type III secretion system (T3SS), exopolysaccharides and many microbeassociated molecular patterns in the rhizobia also play important roles in nodulation and immunity response between rhizobia and legumes. However, the promiscuous hosts like Glycine max and Sophora flavescens can nodulate with various rhizobial species harbouring diverse symbiosis genes in different soils, meaning that the nodulation specificity/efficiency might be mainly determined by the host plants and regulated by the soil conditions in a certain cases. Based on previous studies on rhizobial application, we propose a '1+nÀN' model to promote the function of symbiotic nitrogen fixation (SNF) in agricultural practice, where '1' refers to appreciate rhizobium; '+n' means the addition of multiple trace elements and PGPR bacteria; and 'ÀN' implies the reduction of chemical nitrogen fertilizer. Finally, open questions in the SNF field are raised to future think deeply and researches. Current rhizobial taxonomic outline and use of genome sequence in rhizobial systematicsCurrently, all the symbiotic nitrogen-fixing bacteria associating with legumes are found in the Phylum Proteobacteria, mainly in the Classes Alphaproteobacteria (a-rhizobia) and Betaproteobacteria (b-rhizobia), but maybe also Gammaproteobacteria (c-rhizobia) (Shiraishi et al. 2010) , with about 180 species in 20 genera at the time of writing (Fig. 1). Among them, a-rhizobia are the most common group with a very wide distribution in biogeography and host plants, and beta-rhizobia are also well established with specific legumes though less widely distributed.It is common that symbiotic rhizobia are usually intermingled with nonsymbiotic bacteria at different taxonomic levels. For example, a group of nonsymbiotic bacteria isolated from maize root endosphere, Rhizobium

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Section: Specificity Vs Promiscuity Of Rhizobia and Legumesmentioning

confidence: 99%

Recent development and new insight of diversification and symbiosis specificity of legume rhizobia: mechanism and application

Chen

Wang²,

et al. 2021

J Appl Microbiol

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“…The coding of avocado UniGenes to pattern recognition receptors (PRRs) was performed based on their A. thaliana homologs (Nürnberger & Kemmerling, 2006) and the presence of the LysM motif in some of them (Bozsoki et al, 2020) was confirmed through the Pfam annotation.…”

Section: Identification Of Avocado Immune Receptorsmentioning

confidence: 99%

“…Due to the presence of the LysM domain, it has been speculated that these proteins can recognize glycoproteins and presumably participate in their degradation (Zhang, Cannon & Stacey, 2009). Moreover, recently, in Lotus japonicas, receptors associated with nodulation factors and chitin were found to have a very similar structure but contain two diverging motifs in the LysM1 domain that are necessary for discriminating between immunity and symbiotic functions (Bozsoki et al, 2020).…”

Section: Avocado Immune Receptors Expression During F Kuroshium Interactions and In The Resistosomementioning

confidence: 99%

Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq

Pérez-Torres

Ibarra-Laclette

Hernández-Domínguez

et al. 2021

PeerJ

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Fusarium kuroshium is a novel member of the Ambrosia Fusarium Clade (AFC) that has been recognized as one of the symbionts of the invasive Kuroshio shot hole borer, an Asian ambrosia beetle. This complex is considered the causal agent of Fusarium dieback, a disease that has severely threatened natural forests, landscape trees, and avocado orchards in the last 8 years. Despite the interest in this species, the molecular responses of both the host and F. kuroshium during the infection process and disease establishment remain unknown. In this work, we established an in vitro pathosystem using Hass avocado stems inoculated with F. kuroshium to investigate differential gene expression at 1, 4, 7 and 14 days post-inoculation. RNA-seq technology allowed us to obtain data from both the plant and the fungus, and the sequences obtained from both organisms were analyzed independently. The pathosystem established was able to mimic Fusarium dieback symptoms, such as carbohydrate exudation, necrosis, and vascular tissue discoloration. The results provide interesting evidence regarding the genes that may play roles in the avocado defense response to Fusarium dieback disease. The avocado data set comprised a coding sequence collection of 51,379 UniGenes, from which 2,403 (4.67%) were identified as differentially expressed. The global expression analysis showed that F. kuroshium responsive UniGenes can be clustered into six groups according to their expression profiles. The biologically relevant functional categories that were identified included photosynthesis as well as responses to stress, hormones, abscisic acid, and water deprivation. Additionally, processes such as oxidation-reduction, organization and biogenesis of the cell wall and polysaccharide metabolism were detected. Moreover, we identified orthologues of nucleotide-binding leucine-rich receptors, and their possible action mode was analyzed. In F. kuroshium, we identified 57 differentially expressed genes. Interestingly, the alcohol metabolic process biological category had the highest number of upregulated genes, and the enzyme group in this category may play an important role in the mechanisms of secondary metabolite detoxification. Hydrolytic enzymes, such as endoglucanases and a pectate lyase, were also identified, as well as some proteases. In conclusion, our research was conducted mainly to explain how the vascular tissue of a recognized host of the ambrosia complex responds during F. kuroshium infection since Fusarium dieback is an ambrosia beetle-vectored disease and many variables facilitate its establishment.

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“…Interactions between the host and microbes are shaped by multiple factors associated with host immunity, phylogeny, environmental features (e.g., pH, presence of biotic or abiotic compounds, temperature), diet and nutrient availability (Brinker et al, 2019).…”

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confidence: 99%

Legume tasters: symbiotic rhizobia host preference and smart inoculant formulations

Cangioli

Checcucci

Mengoni

et al. 2021

BioComm

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Mutualistic interactions have great importance in ecology, with genetic information that takes shape through interactions within the symbiotic partners and between the partners and the environment. It is known that variation of the host-associated microbiome contributes to buffer adaptation challenges of the host’s physiology when facing varying environmental conditions. In agriculture, pivotal examples are symbiotic nitrogen-fixing rhizobia, known to contribute greatly to host (legume plants) adaptation and host productivity. A holistic view of increasing crop yield and resistance to biotic and abiotic stresses is that of microbiome engineering, the exploitation of a host-associated microbiome through its rationally designed manipulation with synthetic microbial communities. However, several studies highlighted that the expression of the desired phenotype in the host resides in species-specific, even genotype-specific interactions between the symbiotic partners. Consequently, there is a need to dissect such an intimate level of interaction, aiming to identify the main genetic components in both partners playing a role in symbiotic differences/host preferences. In the present paper, while briefly reviewing the knowledge and the challenges in plant–microbe interaction and rhizobial studies, we aim to promote research on genotype x genotype interaction between rhizobia and host plants for a rational design of synthetic symbiotic nitrogen-fixing microbial communities to be used for sustainably improving leguminous plants yield.

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Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity

Cited by 103 publications

References 52 publications

Recent development and new insight of diversification and symbiosis specificity of legume rhizobia: mechanism and application

Recent development and new insight of diversification and symbiosis specificity of legume rhizobia: mechanism and application

Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq

Legume tasters: symbiotic rhizobia host preference and smart inoculant formulations

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