Ligand Receptor Characteristics of Peptides Encoded by Complementary Nucleic Acids: Implications for a Molecular Recognition Code

Je, Blalock; Kl, Bost

doi:10.1016/b978-0-12-571144-9.50011-9

Cited by 12 publications

(11 citation statements)

References 15 publications

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“…In the present study, EAE CP to the epitope (Rt72-82) was found to be able to bind to the epitope peptide Rt72-82, with an affinity of 3 pM, which is comparable to the affinities of most of the sense/antisense peptide interactions reported by others (from nM to 0.1 mM) (for references, see Blalock and Bost, 1988). This binding was also shown to be specific, because the EAE CP did not bind to a control peptide or BSA and because this binding between the Rt72-82 and EAE CP could be significantly reduced by the pre-incubation of the EAE CP with Rt72-82 in solution before the EAE CP was added to the wells coated with the Rt72-82.…”

Section: Binding Of Eae Cp To Rt72-82 and Gp69-82 Epitope Peptidessupporting

confidence: 82%

Studies on T‐cell receptors involved in experimental autoimmune encephalomyelitis using the complementary peptide recognition approach

Chen

Simmons²,

Willenborg³

et al. 1995

J of Neuroscience Research

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Based upon Blalock's complementary recognition approach, a complementary or antisense peptide (CP) was designed to the experimental autoimmune encephalomyelitis (EAE) epitope peptide, rat myelin basic protein (MBP) peptide 72-82. This peptide (EAE CP) was shown to have some sequence similarities to T-cell receptors (TCR) and MHC II molecules in a sequence homology search. Solid-phase binding assays demonstrated specific and high affinity binding (3 and 4 microM) between the EAE CP and the rat and guinea pig EAE epitope peptides (Rt72-82 and Gp69-82), respectively. This EAE CP was also found to be immunogenic in rats in an ear swelling test for delayed type hypersensitivity (DTH) reactions and an ELISA for antibody responses. However, a rabbit antibody generated to EAE CP was shown to be unable to stain the V beta 8+ EAE susceptible T-cells in immunofluorescence analyses. This EAE CP was also used in attempts to down-regulate EAE and the results showed that prior immunization with EAE CP in complete Freund's adjuvant could not prevent the Lewis rats from developing EAE. Although the data on sense-antisense peptide interaction were positive and the EAE CP was immunogenic, the inability of EAE CP to regulate EAE indicates that the CP approach may not be generally applicable.

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Section: Binding Of Eae Cp To Rt72-82 and Gp69-82 Epitope Peptidessupporting

confidence: 82%

Studies on T‐cell receptors involved in experimental autoimmune encephalomyelitis using the complementary peptide recognition approach

Chen

Simmons²,

Willenborg³

et al. 1995

J of Neuroscience Research

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“…The idea of antigen-antibody complementarity simply proposes that the variable region of an antibody is chemically complementary to its antigen [21,[45][46][47][48].…”

Section: Second Event: An Anti-antibody Responsementioning

confidence: 99%

New insights that link microbes with the generation of antineutrophil cytoplasmic autoantibodies: the theory of autoantigen complementarity

Preston

Pendergraft

Falk

2005

Current Opinion in Nephrology and Hypertension

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“…The idea being that due to the inverse hydropathy and subsequent mirror image of tertiary structure, the sense (5′-3′) and antisense (3′-5′) peptides would bind in specific and predictable manner (24,25). It has been well established that almost regardless of amino acid identity, the tertiary structure of a protein can be reliably predicted through analysis of the pattern of hydrophobic and hydrophilic residues in the primary amino acid sequence (20).…”

Section: Molecular Recognition Theory and Sense/anti-sense Interactionmentioning

confidence: 99%

Molecular recognition theory and sense-antisense interaction therapeutic applications in autoimmunity

Blalock¹

2012

Front Biosci

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Perhaps behind only the understanding of the genetic code in importance is the comprehension of protein sequence and structure in its effect on modern scientific investigation. How proteins are structured and interact dictates a considerable amount of the body's processes in maintaining homeostasis. Unfortunately, in diseases of autoimmunity, these processes are directed against the body itself and most of the current clinical responses are severely lacking. This review addresses current therapeutics involved in the treatment of various autoimmune diseases and details potential future therapeutics designed with a more targeted approach. Detailed in this manuscript is the concept of utilizing peptides possessing an inverse hydropathy to the immunogenic region of proteins to generate anti-idiotypic (anti-Id) and anti-clonotypic T cell receptor (TCR) antibodies (Abs). Theoretically, the anti-Id Abs cross react with Id Abs and negate the powerful machinery of the adaptive immune response with little to no side effects. A series of studies by a number of groups have shown this to be an exciting and intriguing concept that will likely play a role in the future treatment of autoimmune diseases.

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Ligand Receptor Characteristics of Peptides Encoded by Complementary Nucleic Acids: Implications for a Molecular Recognition Code

Cited by 12 publications

References 15 publications

Studies on T‐cell receptors involved in experimental autoimmune encephalomyelitis using the complementary peptide recognition approach

Studies on T‐cell receptors involved in experimental autoimmune encephalomyelitis using the complementary peptide recognition approach

New insights that link microbes with the generation of antineutrophil cytoplasmic autoantibodies: the theory of autoantigen complementarity

Molecular recognition theory and sense-antisense interaction therapeutic applications in autoimmunity

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