Ligand-Mediated cis-Inhibition of Receptor Signaling in the Self-Incompatibility Response of the Brassicaceae

Tantikanjana, Titima; Nasrallah, June B.

doi:10.1104/pp.15.00572

Cited by 11 publications

(15 citation statements)

References 68 publications

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“…Thus, although further research is needed to identify new SC genes, existing markers are already useful for MAS of SC (Xiao et al, 2019). Tantikanjana and Nasrallah (2015) proposed an alternative inducible SC system. It was mentioned earlier that expression of functional S-genes from Arabidopsis lyrata in SC A. thaliana pollen and stigmas causes SI.…”

Section: Ways Of Achieving Sc and Its Role In Hybrid Breedingmentioning

confidence: 99%

“…It was mentioned earlier that expression of functional S-genes from Arabidopsis lyrata in SC A. thaliana pollen and stigmas causes SI. However, Tantikanjana and Nasrallah (2015) discovered that co-expression of both the A. lyrata SRK and SCR genes in A. thaliana stigma epidermal cells leads to ligand-mediated cis-inhibition of SRK and, thus, disrupts SI. This led them to propose two cis-SCR-based strategies for hybrid-seed production.…”

Section: Ways Of Achieving Sc and Its Role In Hybrid Breedingmentioning

confidence: 99%

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Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology

et al. 2020

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Self-incompatibility (SI) mechanisms prevent self-fertilization in flowering plants based on specific discrimination between self-and non-self pollen. Since this trait promotes outcrossing and avoids inbreeding it is a widespread mechanism of controlling sexual plant reproduction. Growers and breeders have effectively exploited SI as a tool for manipulating domesticated crops for thousands of years. However, only within the past thirty years have studies begun to elucidate the underlying molecular features of SI. The specific S-determinants and some modifier factors controlling SI have been identified in the sporophytic system exhibited by Brassica species and in the two very distinct gametophytic systems present in Papaveraceae on one side and in Solanaceae, Rosaceae, and Plantaginaceae on the other. Molecular level studies have enabled SI to SC transitions (and vice versa) to be intentionally manipulated using marker assisted breeding and targeted approaches based on transgene integration, silencing, and more recently CRISPR knock-out of SI-related factors. These scientific advances have, in turn, provided a solid basis to implement new crop production and plant breeding practices. Applications of self-(in)compatibility include widely differing objectives such as crop yield and quality improvement, marker-assisted breeding through SI genotyping, and development of hybrids for overcoming intra-and interspecific reproductive barriers. Here, we review scientific progress as well as patented applications of SI, and also highlight future prospects including further elucidation of SI systems, deepening our understanding of SI-environment relationships, and new perspectives on plant self/non-self recognition.

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Section: Ways Of Achieving Sc and Its Role In Hybrid Breedingmentioning

confidence: 99%

Section: Ways Of Achieving Sc and Its Role In Hybrid Breedingmentioning

confidence: 99%

Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology

et al. 2020

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“…The pollen once in place; on the surface of stigma, the 'female' receptacle extends the tube down to ovary. Here the selection or discrimination of self versus foreign pollen happens at molecular level, where the protein-protein interaction dictates if the pollen tube will extend all the way to the ova, deep inside the stigma, thereby ensuring the fertilization (5,11). Once fertilization is successful the seed forms and the tree starts to make apples.…”

Section: Narration Using Mpm Perceptive Scalementioning

confidence: 99%

A Perceptive Scale Toolkit for Navigating Across Length Scales

Jain¹,

Sirajuddin²

2019

Preprint

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A naked human eye can perceive objects down to a millimeter length. While lenses and microscopes have overcome this limit, the human mind still lacks perspective when navigating conventional scales (1), especially in the range that are less palpable to naked human eye (2,3). This problem is particularly acute in the context of science communication, where the conventional scale bar units facilitate little comprehension regarding the perception for factorial size differences (3). Here we aim to bridge the gap of scale factors and perspectives using a universal toolkit of objects, which can help comprehend the relative change in length dimensions up to 13 orders of magnitude difference. We further have demonstrated the use of such a universal object toolkit as a length perceptive scale by illustrating and narrating biological phenomena. The meter to picometer ‘length perceptive scale’ proposed here has the potential to cover majority of length scales present in the biological realm, and is analogous to the time compression methods widely used in explaining cosmos timeline (4). Our toolkit can also be calibrated according to the users need in their scientific communication and illustrations, which will aid the readers’ benefit in understanding the length scale perception of illustrated phenomenon.

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“…Notably, this localization of the SRK at the plasma membrane and in juxtaposition to the cell wall, which make its extracellular domain accessible to its pollen-derived SCR ligand, is critical for SRK function. Indeed, loss of SI has been observed when SRK targeting to the plasma membrane is disrupted ( Yamamoto et al 2014 ; Tantikanjana and Nasrallah. 2015 ), and when stigmas are treated with 5M NaCl, which causes plasmolysis and retraction of the plasma membrane from the cell wall of epidermal cells ( Rea and Nasrallah.…”

mentioning

confidence: 99%

“…2015 ), and when stigmas are treated with 5M NaCl, which causes plasmolysis and retraction of the plasma membrane from the cell wall of epidermal cells ( Rea and Nasrallah. 2015 ; Tantikanjana and Nasrallah. 2015 ).…”

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confidence: 99%

Activation of Self-Incompatibility Signaling in TransgenicArabidopsis thalianaIs Independent of AP2-Based Clathrin-Mediated Endocytosis

Nishio

Nasrallah

2018

G3 Genes|Genomes|Genetics

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Internalization of plasma membrane (PM)-localized ligand-activated receptor kinases and their trafficking to sorting endosomes have traditionally been viewed as functioning primarily in the down-regulation of receptor signaling, but are now considered to be also essential for signaling by some receptors. A major mechanism for internalization of PM proteins is clathrin-mediated endocytosis (CME). CME is mediated by the Adaptor Protein Complex 2 (AP2), which is involved in interaction of the AP2 μ-adaptin subunit with a tyrosine-based Yxxϕ motif located in the cytoplasmic domain of the cargo protein. In this study, we investigated the role of AP2-mediated CME for signaling by the S-locus receptor kinase (SRK), a protein localized in the PM of stigma epidermal cells, which, together with its pollen coat-localized S-locus cysteine-rich (SCR) ligand, functions in the self-incompatibility (SI) response of the Brassicaceae. Using Arabidopsis thaliana plants that were made self-incompatible by transformation with an A. lyrata-derived SRK/SCR gene pair, we tested the effect on SI of site-directed mutations in each of the two Yxxϕ motifs in SRK and of a CRISPR/Cas9-induced null mutation in the AP2 μ-adaptin gene AP2M. Both in vitro SRK kinase activity and the in planta SI response were abolished by substitution of tyrosine in one of the two Yxxϕ motifs, but were unaffected by elimination of either the second Yxxϕ motif or AP2M function. Thus, AP2-mediated CME is considered to be unnecessary for SRK signaling in the SI response.

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Ligand-Mediated cis-Inhibition of Receptor Signaling in the Self-Incompatibility Response of the Brassicaceae

Cited by 11 publications

References 68 publications

Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology

Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology

A Perceptive Scale Toolkit for Navigating Across Length Scales

Activation of Self-Incompatibility Signaling in TransgenicArabidopsis thalianaIs Independent of AP2-Based Clathrin-Mediated Endocytosis

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