Ligand Binding to the (1 → 3)-β-D-Glucan Receptor Stimulates NFκB Activation, but Not Apoptosis in U937 Cells

Battle, James; Ha, Tuanzhu; Li, Chaunfu; Beffa, Vittorio Della; Rice, Peter J.; Kalbfleisch, John H.; Browder, William; Williams, David L.

doi:10.1006/bbrc.1998.9175

Cited by 93 publications

(61 citation statements)

References 23 publications

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“…However, when comparing each level with the control, macrophages were increased (P < 0.05) at the levels of 0.10, 0.15 and 0.20% inclusion of A. blazei. It is likely that the betaglucans present in the mushroom activated immune functions by increasing the concentration of macrophages, and this is possible only in the presence of the polysaccharide receptors that have been identified on the surfaces of macrophages (BATTLE et al, 1998). Sorimachi et al (2001) concluded that A. blazei is capable of stimulating macrophages in vitro, resulting in the induction of cytokines and the production of nitric oxide.…”

Section: Resultsmentioning

confidence: 99%

Effect of agaricus blazei in the diet of broiler chickens on immunity, serum parameters and antioxidant activity

Fanhani

Murakami

Guerra

et al. 2016

SCA

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The effect of adding Agaricus blazei to the diet of broilers on immunity, serum parameters, and antioxidant activity was evaluated. A total of 840 1-day-old chicks were used, distributed among five levels of a completely randomized design (0.0, 0.05, 0.10, 0.15, and 0.20% A. blazei), with six replications and 28 birds per experimental unit. The weights of the thymus, spleen and cloacal bursa were not influenced (P > 0.05). Leukocytes, macrophages and nitric oxide were unaffected (P > 0.05), but at each supplementation level compared with the control, differences appeared in the percentages of eosinophils and macrophages (P < 0.05) at inclusion levels of 0.10, 0.15 and 0.20%. At 28 days, the antibody titer against Newcastle disease showed a quadratic response (P < 0.05) with supplementation, and from the estimated level of 0.08% the production of antibodies was stimulated; however, the same behavior was not observed (P > 0.05) at 42 days. Hypocholesterolemic effect was demonstrated (P < 0.05), but the serum triglyceride concentration was not affected (P > 0.05). The antioxidant activity of mushroom showed a positive linear effect (P < 0.05) on DPPH capture on day zero of meat cooling. The inclusion of A. blazei in the diet of broilers provided an immunostimulatory activity and hypocholesterolemic effect. Residual compounds with antioxidant activity were present in the meat, which may promote tissue protection of the animal in vivo, making possible the use of A. blazei as a natural additive. Key words: Cholesterol. Free radical. Immunoglobulins. Leukocytes. Mushroom. ResumoAvaliou-se o uso do Agaricus blazei na alimentação de frangos de corte sobre a imunidade, parâmetros séricos e a atividade antioxidante. Utilizou-se 840 pintos distribuídos em delineamento experimental inteiramente casualizado, cinco níveis (0,0; 0,05; 0,10; 0,15 e 0,20%) de Agaricus blazei, seis repetições e 28 aves por unidade experimental. O peso do timo, baço e bolsa cloacal não foi influenciado pelos tratamentos experimentais (P>0,05). Os leucócitos, macrófagos e o óxido nítrico não foram afetados pelos níveis de Agaricus blazei (P>0,05), no entanto a porcentagem de eosinófilo e de macrófagos diferenciaram-se (P<0,05) nos níveis de 0,10, 0,15 e 0,20% em relação ao controle. Aos 28 dias, os níveis de anticorpos contra a doença de Newcastle apresentaram resposta quadrática (P<0,05) à suplementação, e a partir do nível estimado de 0,08% estimulou-se a sua produção, contudo o mesmo

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Section: Resultsmentioning

confidence: 99%

Effect of agaricus blazei in the diet of broiler chickens on immunity, serum parameters and antioxidant activity

Fanhani

Murakami

Guerra

et al. 2016

SCA

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“…Nonopsonic recognition of -glucans by these cells has been ascribed to multiple receptors [2], and indeed a number of -glucan receptors have been identified, including complement receptor type 3, lactosylceramide, scavenger receptor and Dectin-1. However, in these receptors, only Dectin-1 has been clearly shown to have a role in mediating the biological response to -glucans [6].…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective Effects of Paramylon, a .BETA.-1,3-D-Glucan Isolated from Euglena gracilis Z, on Acute Liver Injury Induced by Carbon Tetrachloride in Rats

Sugiyama

Suzuki²,

Mitra³

et al. 2009

J. Vet. Med. Sci.

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ABSTRACT. Paramylon is a -(1-3)-D-glucan isolated from Euglena gracilis Z. This study was designed to evaluate the protective effects of paramylon on liver injury induced by carbon tetrachloride (CCl 4 ) in rats. Wistar stain male rats were orally administered paramylon (500, 1,000 and 2,000 mg/kg body weight) before treatment with a single intraperitoneal dose of 50% CCl 4 (2 ml/kg body weight). The rats were sacrificed 24 hr later, and blood samples were collected for assay of serum biochemical parameters. The livers were excised to evaluate the activity of antioxidant enzymes. Histopathological examination of the livers was also performed. The results showed that the treatment of paramylon prevented elevation of the serum levels of hepatic enzyme markers and inhibited fatty degeneration and hepatic necrosis induced by CCl 4 . Pre-administration of paramylon reduced the liver apoptotic index. The treatment of paramylon recovered reductions of activity of hepatic superoxide dismutase, catalase and glutathione peroxidase induced by CCl 4 . These results demonstrate that paramylon exhibits protective action on acute hepatic injury induced by CCl 4 via an antioxidative mechanism. To the best of our knowledge, this is the first report of a hepatoprotective effect based on the antioxidative action of paramylon.

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“…Although any or all of these conclusions may be valid, they also presented a paradox, in that glucans are well known immune stimulators (30). There is a mild inflammatory response associated with glucan-induced immune stimulation (31,32). The pathophysiology of sepsis/septic shock suggests that after severe injury or infectious challenge the host responds by overexpressing inflammatory mediators resulting in a systemic inflammatory response that may produce severe shock, multiorgan failure, and death (1).…”

Section: Discussionmentioning

confidence: 99%

Modulation of the Phosphoinositide 3-Kinase Pathway Alters Innate Resistance to Polymicrobial Sepsis

Williams¹,

Li²,

Ha³

et al. 2004

The Journal of Immunology

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154

162

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We examined the effect of modulating phosphoinositide 3-kinase (PI3K) activity in a murine model of cecal ligation and puncture-induced polymicrobial sepsis. Inhibition of PI3K activity with wortmannin increased serum cytokine levels and decreased survival time in septic mice. We have reported that an immunomodulator, glucan phosphate, induces protection in murine polymicrobial sepsis. We observed that glucan stimulated tissue PI3K activity, which positively correlated with increased survival in septic mice. We investigated the effect of PI3K inhibition on survival in septic mice treated with glucan. Treatment of mice with the PI3K inhibitors, wortmannin and LY294002, completely eliminated the protective effect of glucan, indicating that protection against septic mortality was mediated through PI3K. Inhibition of PI3K resulted in increased serum levels of IL1-β, IL-2, IL-6, IL-10, IL-12, and TNF-α in septic mice. Apoptosis is thought to play a central role in the response to septic injury. We observed that inhibition of PI3K activity in septic mice resulted in increased splenocyte apoptosis and a change in the anatomic distribution of splenocyte apoptosis. We conclude that PI3K is a compensatory mechanism that suppresses proinflammatory and apoptotic processes in response to sepsis and/or inflammatory injury. Thus, PI3K may play a pivotal role in the maintenance of homeostasis and the integrity of the immune response during sepsis. We also observed that glucan phosphate decreased septic morbidity and mortality through a PI3K-dependent mechanism. This suggests that stimulation of the PI3K pathway may be an effective approach for preventing or treating sepsis and/or septic shock.

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Ligand Binding to the (1 → 3)-β-D-Glucan Receptor Stimulates NFκB Activation, but Not Apoptosis in U937 Cells

Cited by 93 publications

References 23 publications

Effect of agaricus blazei in the diet of broiler chickens on immunity, serum parameters and antioxidant activity

Effect of agaricus blazei in the diet of broiler chickens on immunity, serum parameters and antioxidant activity

Hepatoprotective Effects of Paramylon, a .BETA.-1,3-D-Glucan Isolated from Euglena gracilis Z, on Acute Liver Injury Induced by Carbon Tetrachloride in Rats

Modulation of the Phosphoinositide 3-Kinase Pathway Alters Innate Resistance to Polymicrobial Sepsis

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