Ligament regeneration using a knitted silk scaffold combined with collagen matrix

Chen, Xiao; Qi, Yiying; Wang, Linlin; Yin, Zi; Yin, Guoli; Zou, Xiaohui; Ouyang, Hongwei

doi:10.1016/j.biomaterials.2008.05.017

Cited by 196 publications

(175 citation statements)

References 44 publications

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“…The knitted silk was fabricated as described previously [13]. Briefly, the knitted scaffold was fabricated using 12 yarns (1 filament/yarn) of silk fibers on a knitting machine.…”

Section: Scaffold Fabricationmentioning

confidence: 99%

“…Insoluble type I collagen was derived from pig Achilles' tendon using neutral salt and dilute acid extractions with enzyme treatment [13,23]. Type III collagen was removed by centrifugation after salt fractionation, and type I collagen was drawn off the lower portion.…”

Section: Scaffold Fabricationmentioning

confidence: 99%

“…In particular, type I collagen is the major component of tendon extracellular matrix, and has been extensively utilized in tendon tissue engineering [12]. Nevertheless, regenerated tendon tissue within sponge collagen scaffold is disordered and irregular, which can have a negative impact on the biomechanical function of the regenerated tendon [13]. Hence, it is imperative to further improve the collagen scaffold utilized for tendon repair.…”

Section: Introductionmentioning

confidence: 99%

“…Because pure collagen scaffold exhibit poor mechanical properties in vivo, it is necessary to combine it with a material that possesses good mechanical strength [13]. Silk is a FDA -approved material, which has been widely utilized in tissue engineering due to its good biocompatibility and mechanical strength [21].…”

Section: Introductionmentioning

confidence: 99%

“…The aim of this study is to fabricate a macroporous 3D aligned collagen-silk scaffold with optimal pore size (several hundred micrometers), adequate porosity (higher than 90%) [20], favorable inductivity (enhancing tenogenic differentiation) and sufficient mechanical strength (no tear after implantation in vivo) [13], and to investigate its effect on tendon remodeling during functional shoulder repair in a rabbit massive rotator cuff defect model.…”

Section: Introductionmentioning

confidence: 99%

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Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

Zheng¹,

Ran

Chen

et al. 2017

Acta Biomaterialia

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a b s t r a c tRotator cuff tear is one of the most common types of shoulder injuries, often resulting in pain and physical debilitation. Allogeneic tendon-derived decellularized matrices do not have appropriate pore size and porosity to facilitate cell infiltration, while commercially-available synthetic scaffolds are often inadequate at inducing tenogenic differentiation. The aim of this study is to develop an advanced 3D aligned collagen/silk scaffold (ACS) and investigate its efficacy in a rabbit massive rotator cuff tear model. ACS has similar 3D alignment of collagen fibers as natural tendon with superior mechanical characteristics. Based on ectopic transplantation studies, the optimal collagen concentration (10 mg/ml), pore diameter (108.43 ± 7.25 lm) and porosity (97.94 ± 0.08%) required for sustaining a stable macro-structure conducive for cellular infiltration was determined. Within in vitro culture, tendon stem/progenitor cells (TSPCs) displayed spindle-shaped morphology, and were well-aligned on ACS as early as 24 h. TSPCs formed intercellular contacts and deposited extracellular matrix after 7 days. With the in vivo rotator cuff repair model, the regenerative tendon of the ACS group displayed more conspicuous native microstructures with larger diameter collagen fibrils (48.72 ± 3.75 vs. 44.26 ± 5.03 nm) that had better alignment and mechanical properties (139.85 ± 49.36 vs. 99.09 ± 33.98 N) at 12 weeks post-implantation. In conclusion, these findings demonstrate the positive efficacy of the macroporous 3D aligned scaffold in facilitating rotator cuff tendon regeneration, and its practical applications for rotator cuff tendon tissue engineering. Statement of SignificanceMassive rotator cuff tear is one of the most common shoulder injuries, and poses a formidable clinical challenge to the orthopedic surgeon. Tissue engineering of tendon can potentially overcome the problem. However, more efficacious scaffolds with good biocompatibility, appropriate pore size, favorable inductivity and sufficient mechanical strength for repairing massive rotator cuff tendon injuries need to be developed. In this study, we developed a novel macroporous 3D aligned collagen/silk scaffold, and demonstrated that this novel scaffold enhanced the efficacy of rotator cuff tendon regeneration by inducing aligned supracellular structures similar to natural tendon, which in turn enhanced http://dx

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“…The knitted silk was fabricated as described previously [13]. Briefly, the knitted scaffold was fabricated using 12 yarns (1 filament/yarn) of silk fibers on a knitting machine.…”

Section: Scaffold Fabricationmentioning

confidence: 99%

Section: Scaffold Fabricationmentioning

confidence: 99%