Lifetime of Phosphorescence from Nanoparticles Yields Accurate Measurement of Concentration of Oxygen in Microdroplets, Allowing One To Monitor the Metabolism of Bacteria

Horka, Michał; Sun, Shiwen; Ruszczak, Artur; Garstecki, Piotr; Mayr, Torsten

doi:10.1021/acs.analchem.6b03758

Cited by 26 publications

(40 citation statements)

References 43 publications

(65 reference statements)

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“…We can further distinguish the systems that use integrated on-chip valves 36 or off-chip actuation. 10,27,28,35,38,39 Both approaches have their strengths and weaknesses. On-chip valves made of elastomer have very small dead-volumes and thus can handle minute subnanoliter volumes 36 and their capabilities for the execution of multiplex assays can be further improved by efficient multiplexing via microfluidics large-scale integration.…”

Section: Automated Droplet Microfluidics For the Generation Of Trainsmentioning

confidence: 99%

Controlled droplet microfluidic systems for multistep chemical and biological assays

2017

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Droplet microfluidics is a relatively new and rapidly evolving field of science focused on studying the hydrodynamics and properties of biphasic flows at the microscale, and on the development of systems for practical applications in chemistry, biology and materials science. Microdroplets present several unique characteristics of interest to a broader research community. The main distinguishing features include (i) large numbers of isolated compartments of tiny volumes that are ideal for single cell or single molecule assays, (ii) rapid mixing and negligible thermal inertia that all provide excellent control over reaction conditions, and (iii) the presence of two immiscible liquids and the interface between them that enables new or exotic processes (the synthesis of new functional materials and structures that are otherwise difficult to obtain, studies of the functions and properties of lipid and polymer membranes and execution of reactions at liquid-liquid interfaces). The most frequent application of droplet microfluidics relies on the generation of large numbers of compartments either for ultrahigh throughput screens or for the synthesis of functional materials composed of millions of droplets or particles. Droplet microfluidics has already evolved into a complex field. In this review we focus on 'controlled droplet microfluidics' - a portfolio of techniques that provide convenient platforms for multistep complex reaction protocols and that take advantage of automated and passive methods of fluid handling on a chip. 'Controlled droplet microfluidics' can be regarded as a group of methods capable of addressing and manipulating droplets in series. The functionality and complexity of controlled droplet microfluidic systems can be positioned between digital microfluidics (DMF) addressing each droplet individually using 2D arrays of electrodes and ultrahigh throughput droplet microfluidics focused on the generation of hundreds of thousands or even millions of picoliter droplets that cannot be individually addressed by their location on a chip.

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Section: Automated Droplet Microfluidics For the Generation Of Trainsmentioning

confidence: 99%

Controlled droplet microfluidic systems for multistep chemical and biological assays

2017

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“…inferior to properties of glass, but still acceptable for many applications (Jeong et al, 2016). The most common thermoplastics used for the fabrication of microfluidic chips are the following materials: polycarbonate (PC) (Ogończyk et al, 2010), polymethylmethacrylate (PMMA) (Conchouso et al, 2014), cyclic-olefin copolymers (COC) (Stachowiak et al, 2007), polystyrene (PS) (Li et al, 2012), polytetrafluoroethylene (PTFE, also known under the brand name Teflon ® ), fluorinated ethylene propylene (FEP) (Horka et al, 2016). Prototyping in thermoplastics is usually done by i) micromilling the channels in the plate and bonding it to another plate (Ogończyk et al, 2010); or by ii) 3D printing the appropriate polymer, e.g.…”

Section: Prototyping Methodsmentioning

confidence: 99%

Droplet Microfluidics as a Tool for the Generation of Granular Matters and Functional Emulsions

Opalski

Kamiński

Garstecki

2019

KONA

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Emulsion-a liquid dispersed in another liquid-is in many respects very similar to granular matter. In the early 2000s a new technology-droplet microfluidics-began emerging from the wider field of microfluidics. Droplet microfluidics was quickly established as a discipline of science and engineering and has been used for the generation of highly uniform emulsions. The last few years have brought significant advances to the field, directed towards a wide range of applications in material sciences-from synthesis of nanoparticles in droplets to assembling complex droplets and using droplets as templates for ordered materials, with applications in food, cosmetic and diagnostic industries. Droplet microfluidic platforms are also successfully used as analytical tools in molecular biology and biochemistry, in e.g. high-throughput screening, digital assays, encapsulation of single cells, sequencing technologies, and in point-of-care diagnostic applications. This article provides an accessible overview of the physical phenomena observed in multiphase flow at the microscale and the techniques in droplet microfluidics systems. We also present the most interesting applications and potential further directions of research in this fascinating young field of science and engineering.

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“…Oxygen quenching of phosphorescence has been widely studied, and a wide assortment of sensors for different analytes like pH, CO2, orthophosphate, chloride, 1-butylamine, antibacterial oxolinic acid and antibiotic flumequine have been developed based on measurement of changes in O2 levels detected by resorting to phosphorimetric oxygen sensing [7]. In this context, phosphorimetric nanoparticles have been evaluated for monitoring the level of oxygen dissolved in microdroplets [63]. antennae in hydrogel nanoparticles [64].…”

Section: Direct Sensing Of Small Molecules and Ionsmentioning

confidence: 99%

Functionalized phosphorescent nanoparticles in (bio)chemical sensing and imaging – A review

Llano-Suárez

García-Cortés

Fernández-Argüelles

et al. 2019

Analytica Chimica Acta

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Inorganic nanoparticles are a fascinating class of materials which promise great potential in numerous fields, including optical (bio)sensing. Many different kinds of such nanoparticles have been widely used for fluorescent sensing and imaging due to the different merits of fluorescent nanoparticles compared to molecular fluorophores.Progress made in the rational design of nanomaterials also allowed the synthesis of hybrid phosphorescent nanoparticles, that finds growing applications in sensing due to the combination of the interesting size-and shape-dependent properties of nanomaterials with a phosphorescence-type emission.In this review, we intend to highlight some of progress made in this active research area and update the database of various phosphorescent nanoparticles-based sensors on the basis of different sensing targets of interest in environmental, industrial and biomedical areas.Following an introduction and a discussion of merits of the synergy between nanomaterials and phosphorescence detection as compared to molecular luminophores the article assesses the kinds and specific features of nanomaterials often used in phosphorescence sensing. Specific examples on the use of phosphorescence nanoparticles in chemical sensing and bioimaging are given next. A final section intends to provide an overview of the prospects of such type of nanomaterials in the design of future devices for analytical chemistry.

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Lifetime of Phosphorescence from Nanoparticles Yields Accurate Measurement of Concentration of Oxygen in Microdroplets, Allowing One To Monitor the Metabolism of Bacteria

Cited by 26 publications

References 43 publications

Controlled droplet microfluidic systems for multistep chemical and biological assays

Controlled droplet microfluidic systems for multistep chemical and biological assays

Droplet Microfluidics as a Tool for the Generation of Granular Matters and Functional Emulsions

Functionalized phosphorescent nanoparticles in (bio)chemical sensing and imaging – A review

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